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pU6-Osp.gRNA-MS2in

Plasmid

#229772

Purpose

Contains the U6 promoter and an optimized gRNA backbone inserted with two copies of the MS2 stem loop.

Depositor

Yujia Cai

Article

Ling et al Nat Nanotechnol. 2025 Apr;20(4):543-553.

Insert

gRNA cassette with two MS2 stem loops

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (-) hYAP 1-1gamma

Plasmid

#124141

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pRGEN-CMV-10XGCN4-L1-dCjCas9 S-D

Plasmid

#169912

Purpose

Expression of a SunTag-dCjCas9 fusion protein in which 10 copies of the GCN4 peptide are fused to the N terminus of dCjCas9.

Depositor

Zhili Rong

Article

Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

Insert

dCjCas9

Use

CRISPR

Tags

10XGCN4, 2xSV40NLS, and SV40NLS-HA

Expression

Mammalian

Mutation

D8A, H559A in cjCas9

Promoter

CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe-TetCMV-puro-mCherry-PA-Rac1

Plasmid

#22035

Purpose

Expression of photoactivatable Rac1

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mCherry-PA-Rac1 (RAC1 Human, Avena sativa (oat))

Use

Retroviral

Expression

Mammalian

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Promoter

Tet-CMV

Available Since

Sept. 11, 2009

Availability

Academic Institutions and Nonprofits only

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pHIV-BRAFV600E-mOrange2

Plasmid

#110732

Purpose

Mammalian Expression of BRAFV600E IRES H2B-mOrange2

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

BRAFV600E IRES H2B-mOrange2 (BRAF Human)

Use

Lentiviral

Expression

Mammalian

Mutation

BRAF V600E

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgSLC7A11/xCT-2

Plasmid

#161819

Purpose

knock out SLC7A11/xCT in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

Insert

solute carrier family 7 member 11 (SLC7A11 Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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Kif1bβ-pcDNA3

Plasmid

#22534

Depositor

William Kaelin

Article

Schlisio et al Genes Dev. 2008 Apr 1. 22(7):884-93.

Insert

Kif1bβ (KIF1B Human)

Tags

FLAG

Expression

Mammalian

Mutation

wt cDNA

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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GPS-MYC

Plasmid

#160130

Purpose

A reporter that monitors MYC protein expression.

Depositor

Channing Der

Article

Blake et al Sci Signal. 2019 Jul 16;12(590). pii: 12

Insert

MYC (MYC Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pExp-xMBP-ACE2-CHis

Plasmid

#194998

Purpose

Production of ACE2 extracellular domain

Depositor

Marko Hyvönen

Article

Harman et al J Med Chem. 2023 Jul 27;66(14):9881-9893

Insert

ACE2 (ACE2 Human)

Tags

His8 and xMBP

Expression

Bacterial

Promoter

T7lac

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-puro-N-Flag-h-SNRNP40

Plasmid

#134254

Purpose

Lentivector encoding Flag-tagged human SNRNP40

Depositor

Bruce Beutler

Article

Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

Insert

SNRP40 (SNRNP40 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF1a

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pX330_CDC45

Plasmid

#244243

Purpose

Expresses SpCas9 and a sgRNA targeting the human CDC45 loci for knock-in.

Depositor

Kazuhiro Maeshima

Article

Minami et al Sci Adv. 2025 Mar 28;11(13):eadu8400. do

Insert

sgRNA targeting human CDC45 (CDC45 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgCYFIP1-2

Plasmid

#210138

Purpose

knock out CYFIP1 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Cytoplasmic FMR1-interacting protein 1 (CYFIP1 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-PA-Rac1-C450A

Plasmid

#22028

Purpose

Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))

Tags

6xHis and mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

LOV(Leu404-Leu546, C450A). Rac1 starts at Ile4 an…

Promoter

CMV, p10

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

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pLX304 ERG

Plasmid

#118625

Purpose

Overexpresses ERG protein

Depositor

William Hahn

Article

Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

Insert

ERG (33-479) (ERG Human)

Use

Lentiviral

Tags

None and none

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pB-Halo,IRES-eGFP

Plasmid

#164519

Purpose

All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible GFP and constitutive rtTA and neomycin resistance)

Depositor

Mitchell Guttman

Article

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTRE3G-GSX2

Plasmid

#96964

Purpose

Dox-inducible expression of Gsx2 in mammalian cells

Depositor

Elena Cattaneo

Article

Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Insert

GSX2 (Gsx2 Mouse)

Expression

Mammalian

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P2-MDM2 (17-125)

Plasmid

#62063

Purpose

Expression of human MDM2 (17-125) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

MDM2 (MDM2 Human)

Tags

GST

Expression

Bacterial

Mutation

contains residues 17-125

Promoter

tac

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-hGPR56-IRES-GFP

Plasmid

#52297

Purpose

Expresses human GPR56 and GFP in mammalian cells.

Depositor

Christopher A Walsh

Article

Bae et al Science. 2014 Feb 14;343(6172):764-8. do

Insert

Human GPR56 coding sequence (ADGRG1 Human)

Expression

Mammalian

Promoter

CAG promoter

Available Since

April 23, 2014

Availability

Academic Institutions and Nonprofits only

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pGM234

Plasmid

#220177

Purpose

Expresses exogenous wild-type NAC complex members (NACA and BTF3)

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

NACA and BTF3 (NACA, BTF3 Human)

Use

Lentiviral

Tags

3xFLAG (NACA); 6xHis (BTF3)

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-human cGAS-HA

Plasmid

#186842

Purpose

Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Human cGAS with C-terminal HA tag (Adamts1 Human)

Use

Retroviral

Tags

HA

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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