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Showing: 2201 - 2250 of 11688 results

  4. pcDNA3.1.Gα12-LgB115

    Plasmid
    #134363
    Purpose
    Nanoluc complementation assay. Expression of Gα12 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 115 and 116 of Gα12.Addition of the HA epitope at N terminus of Gα12.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gα12-LgB115 (GNA12 Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134363-plasmid-map-sequence-id-306384

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  8. pCDNA3.1 Bach1 S990A

    Plasmid
    #73643
    Purpose
    Mammalian expression of Bach1 S990A mutant (BRCA binding mutant).
    Depositor
    Sharon Cantor
    Insert
    Bach1 (BACH1 Human)
    Use
    Tags
    His and Myc
    Expression
    Mammalian
    Mutation
    S990A (BRCA binding mutant)
    Promoter
    CMV
    Available since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only

  20. pCDNA5_FRT_TO_TurboID-mCherry-Cry2

    Plasmid
    #166504
    Purpose
    Expression of your protein of interest fused with TurboID and mcherry-CRY2 for optogenetic activation, Flp-In T-Rex system. Search for client proteins within light-induced biomolecular condensates.
    Depositor
    Angelos Constantinou
    Article
    Frattini et al Mol Cell. 2021 Jan 16. pii: S1097-2765(2
    Insert
    None
    Use
    Tags
    TurboID and mCherry-CRY2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    166504-plasmid-map-sequence-id-328214

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  21. pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX

    Plasmid
    #52421
    Purpose
    Expresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherry
    Depositor
    Bertrand Collet
    Insert
    Fusion NLS-EGFP-P2A-Cherry-CAAX
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    52421-plasmid-map-sequence-id-81252

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  25. pcDNA3-GFP-MMP7

    Plasmid
    #11989
    Depositor
    Steven Johnson
    Insert
    MMP7 (MMP7 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 25, 2007
    Availability
    Academic Institutions and Nonprofits only
    11989-plasmid-map-sequence-id-133484

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  34. pcDNA3-endo-GsCT

    Plasmid
    #205516
    Purpose
    Endosome-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).
    Depositor
    Jin Zhang
    Article
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Insert
    endo-GsCT (GNAS Synthetic, Human)
    Use
    Tags
    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    205516-plasmid-map-sequence-id-409476

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  35. pcDNA3-RIIα-Dronpa

    Plasmid
    #182331
    Purpose
    PKA RIIα subunit-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    RIIalpha-Dronpa (PRKAR2A Human)
    Use
    Tags
    Full-length human PKA type 2alpha regulatory subu…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182331-plasmid-map-sequence-id-363196

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  39. pcDNA3-Mcm5-mCer

    Plasmid
    #211393
    Purpose
    mammalian expression of human Mcm5 C-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    Mcm5 (MCM5 Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211393-plasmid-map-sequence-id-420429

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  42. pcDNA3-DAKAP-μDpTT

    Plasmid
    #182324
    Purpose
    Mitochondria-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    DAKAP-μDronpa-TagRFP-T
    Use
    Tags
    N-terminal 30 amino acids from DAKAP1
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Available since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182324-plasmid-map-sequence-id-362788

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  44. pcDNA3.1-ccdB-TwinStrep

    Plasmid
    #214988
    Purpose
    Gateway compatible empty vector (transient mammalian expression) with a C-terminal TwinStrep tag
    Depositor
    Wade Harper
    Type
    Empty backbone
    Use
    Tags
    TwinStrep
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214988-plasmid-map-sequence-id-440022

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  48. pcDNA.3.1.ADRB2-NP

    Plasmid
    #134376
    Purpose
    Nanoluc complementation assay. Expression of adrenoceptor beta 2 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of signal sequence and Flag epitope at N terminus of ADRB2.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    ADRB2-NP (ADRB2 Human)
    Use
    Tags
    Flag, natural peptide of nanoluciferase, and sign…
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available since
    Aug. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134376-plasmid-map-sequence-id-306396

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Showing: 2201 - 2250 of 11688 results