We narrowed to 7,376 results for: ESP

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pMM704

Plasmid

#68891

Purpose

IPTG-inducible CRISPRi vector targeting BT1854, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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BRCA1 A10.2 gRNA

Plasmid

#90549

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRCA1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BRCA1 (Guide Designation A10.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCE27

Plasmid

#174405

Purpose

C. auris LEUpOUT NAT marker gRNA expression construct. Use with pCE35 CAS9 expression construct.

Depositor

Clarissa Nobile

Article

Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

Insert

NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2

Use

CRISPR

Expression

Bacterial

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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VEGFR2-YFP

Plasmid

#108853

Purpose

Encodes for human VEGFR2 fluorescently labeled with eYFP on the C-Terminus via a 3 amino acid (GGS) flexible linker

Depositor

Kalina Hristova

Article

Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554

Insert

KDR (KDR Human)

Tags

Labeled with eYFP on the C-Terminus via a 3 amino…

Expression

Mammalian

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-ataxin-3

Plasmid

#89975

Purpose

Expresses GFP-tagged ataxin-3

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hsGFP

Plasmid

#64912

Purpose

Encodes a Fluorescent Reporter for Hydrogen Sulfide in Mammalian Cells

Depositor

Huiwang Ai

Article

Chen et al Biochemistry. 2014 Sep 23;53(37):5966-74

Insert

hsGFP

Tags

6xHis

Expression

Mammalian

Mutation

Chromophore tyrosine of the protein mutated to TA…

Promoter

CMV

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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Mito-TurboID

Plasmid

#200960

Purpose

Transiently expresses mitochondria localized TurboID with V5 tags

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

mito-TurboID

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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FUGW-Cytosol-TurboID

Plasmid

#200961

Purpose

3rd gen lentiviral plasmid for expressing cytosol localized TurboID with EGFP

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

Cytosol-TurboID

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pX462-hPRKAA2-gRNA_B

Plasmid

#74377

Purpose

gRNA_B to knockout human AMPK alpha 2 using Cas9n

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

human AMPK alpha 2 exon1 gRNA (PRKAA2 Human)

Use

CRISPR

Promoter

U6

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-p67MBP

Plasmid

#42

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

p67 myb binding protein (Mybbp1a Mouse)

Tags

myc

Expression

Mammalian

Available Since

July 21, 2005

Availability

Academic Institutions and Nonprofits only
pCGN-ELK-1 S383A

Plasmid

#27160

Purpose

For mammalian expression of human Elk-1 with an HA tag and a mutation in the MAPK phosphorylation site of the transcriptional activation domain

Depositor

Ron Prywes

Article

Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

Insert

ELK1 (ELK1 Human)

Tags

HA

Expression

Mammalian

Mutation

TAD mutant: S383A

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pdestMB14_sur5p-dCas9-VP64

Plasmid

#177788

Purpose

Overexpression of dCas9-VP64 in C. elegans

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

dCas9-VP64

Expression

Worm

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET_RGG-RGG

Plasmid

#124941

Purpose

Expresion of RGG-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

His

Expression

Bacterial

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-SCR-sgRNA-A

Plasmid

#177811

Purpose

Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

Scrambled sgRNA targeting sequences

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_C_Myc_DDK_IRES_Puro_PRKRA

Plasmid

#106113

Purpose

Lentiviral expression of PRKRA

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Use

Lentiviral

Tags

DDK (FLAG) and Myc

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-f:PGC1-3D

Plasmid

#47

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

PGC-1a (Ppargc1a Mouse)

Tags

flag

Expression

Mammalian

Mutation

Amino acids 262, 265, and 298 have been changed t…

Available Since

Oct. 4, 2005

Availability

Academic Institutions and Nonprofits only
R777-E111 Hs.IRS2

Plasmid

#70395

Purpose

Gateway ORF clone of codon optimized human IRS2 [NM_003749] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

IRS2 (IRS2 Human)

Use

Gateway Cloning

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-mHRI-FLAG-IP

Plasmid

#101791

Purpose

Retroviral vector encoding C-terminally FLAG tagged mouse HRI

Depositor

Seiichi Oyadomari

Article

Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

Insert

Hri (Eif2ak1 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Promoter

LTR

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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ALPaGA

Plasmid

#175272

Purpose

Lactate biosensor in anaerobic conditions

Depositor

Jerome Bonnet

Article

Zuniga et al ACS Synth Biol. 2021 Dec 1. doi: 10.1021

Insert

LldR_Synthetic_promoter_sfGFP

Expression

Bacterial

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCE35

Plasmid

#174409

Purpose

C. auris LEUpOUT NAT marker CAS9 expression construct. Use with pCE27 gRNA expression construct.

Depositor

Clarissa Nobile

Article

Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

Insert

C. auris LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2

Use

CRISPR

Expression

Bacterial

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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