We narrowed to 2,461 results for: T2A

1. SOX9-T2A-NLS-tdTomato-PGK-Puro

   Plasmid

   #89993

   Purpose

   Donor template for generation of SOX9-ntdTomato reporter cell lines

   Depositor

   Timo Otonkoski

   Insert

   SOX9-T2A-NLS-TdTomato-PGK-Puro (SOX9 Human)
   

   Use

   CRISPR

   Promoter

   none

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti spCas9 T2A mNeonGreen P2A puro

   Plasmid

   #122183

   Purpose

   The plasmid codes for a Flag-spCas9 protein, a mNeonGreen fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

   Depositor

   Raphael Gaudin

   Inserts

   spCas9 (NEWENTRY )
   mNeonGreen
   

   Use

   Lentiviral

   Tags

   T2A

   Expression

   Mammalian

   Available Since

   April 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-ProtC-AU1

   Plasmid

   #162111

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to StrepTagII-ProtC-AU1
   

   Use

   Lentiviral

   Tags

   StrepTagII-ProtC-AU1

   Mutation

   WT

   Promoter

   EF1A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEpicVector3G_Puro-T2A-EGFP-StrepTagII-ProtC-FLAG

   Plasmid

   #162090

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to StrepTagII-ProtC-FLAG
   

   Use

   Lentiviral

   Tags

   StrepTagII-ProtC-FLAG

   Mutation

   WT

   Promoter

   EF1A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEpicVector3G_Puro-T2A-EGFP-ProtC-FLAG-AU1

   Plasmid

   #162097

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to ProtC-FLAG-AU1
   

   Use

   Lentiviral

   Tags

   ProtC-FLAG-AU1

   Mutation

   WT

   Promoter

   EF1A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEpicVector3G_Puro-T2A-EGFP-VSVg-StrepTagII-AU1

   Plasmid

   #162082

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to VSVg-StrepTagII-AU1
   

   Use

   Lentiviral

   Tags

   VSVg-StrepTagII-AU1

   Mutation

   WT

   Promoter

   EF1A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO

   Plasmid

   #128755

   Purpose

   Expresses CYRANO gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   CYRANO sgRNA (OIP5-AS1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS-KS-attB2-SA(0)-T2A-LexA::QFAD-Hsp70

   Plasmid

   #62947

   Purpose

   Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 0

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-LexA::QFAD-Hsp70
   

   Available Since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. p194-RV-CAG-dio-mScarlet-T2A-Brn2

   Plasmid

   #204741

   Purpose

   Conditional expression of target gene Brn2 with fluorescent reporter mScarlet

   Depositor

   Lynette Lim

   Article

   Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

   Insert

   Brn2
   

   Use

   Retroviral

   Promoter

   CAG

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEpicVector3mC_Puro-T2A-mCherry-VSVg-StrepTagII-AU1

    Plasmid

    #162102

    Purpose

    mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; mCherry linked to VSVg-StrepTagII-AU1
    

    Use

    Lentiviral

    Tags

    VSVg-StrepTagII-AU1

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEpicVector3mC_Puro-T2A-mCherry-VSVg-ProtC-FLAG

    Plasmid

    #162104

    Purpose

    mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; mCherry linked to VSVg-ProtC-FLAG
    

    Use

    Lentiviral

    Tags

    VSVg-ProtC-FLAG

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-ProtC-FLAG

    Plasmid

    #162110

    Purpose

    mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; mCherry linked to StrepTagII-ProtC-FLAG
    

    Use

    Lentiviral

    Tags

    StrepTagII-ProtC-FLAG

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEpicVector3G_Puro-T2A-EGFP-VSVg-ProtC-AU1

    Plasmid

    #162085

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to VSVg-ProtC-AU1
    

    Use

    Lentiviral

    Tags

    VSVg-ProtC-AU1

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEpicVector3G_Puro-T2A-EGFP-VSVg-FLAG-AU1

    Plasmid

    #162088

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to VSVg-FLAG-AU1
    

    Use

    Lentiviral

    Tags

    VSVg-FLAG-AU1

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEpicVector3G_Puro-T2A-EGFP-StrepTagII-FLAG-AU1

    Plasmid

    #162094

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to StrepTagII-FLAG-AU1
    

    Use

    Lentiviral

    Tags

    StrepTagII-FLAG-AU1

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEpicVector3G_Puro-T2A-EGFP-VSVg-StrepTagII-FLAG

    Plasmid

    #162081

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to VSVg-StrepTagII-FLAG
    

    Use

    Lentiviral

    Tags

    VSVg-StrepTagII-FLAG

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19-Top2A-5X Gly-Venus-T2A-Hygro

    Plasmid

    #211115

    Purpose

    For tagging the C-term of Top2A with Venus

    Depositor

    Job Dekker

    Article

    Hildebrand et al Mol Cell. 2024 Mar 13:S1097-2765(24)0014

    Inserts

    5' C-term Top2A homology arm (TOP2A Human)
    Venus
    T2A
    Blastocidin Resistance
    5' C-term Top2A homology arm (TOP2A Human)
    

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    n/a

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC19_T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP

    Plasmid

    #195503

    Purpose

    SOX17 donor plasmid to generate locus specific C-terminal integration of H2B-mCitrine into the human SOX17-locus

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    SOX17(Ex2)-T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP_SOX17(3'UTR) (SOX17 Human)
    

    Use

    CRISPR; Donor vector

    Tags

    T2A-H2B-mCitrine

    Expression

    Mammalian

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. KL157 - pBlueScript II SK-Grb10_HA-T2A-eGFP-SV40PolyA

    Plasmid

    #232935

    Purpose

    Donor plasmid for knock-in of T2A-eGFP-SV40pA at the mouse Grb10 locus. Used with PX459-sgRNA048_Grb10 sgRNA.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    T2A-eGFP-SV40pA with Grb10 homology arms
    

    Use

    Mouse Targeting

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5

    Plasmid

    #97044

    Purpose

    Expresses LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells. *Please see notes below on P2A cleaving efficiency.

    Depositor

    George Daley

    Article

    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

    Insert

    Ligand Dependent Nuclear Receptor Corepressor, Runt-related transcription factor 1, ETS-related gene
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Tet on

    Available Since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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