We narrowed to 980 results for: trna

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pEvoltac-EcW-TGA-h14

Plasmid

#218764

Purpose

encodes EcWRS-h14 and EcW-tRNA-TGA

Depositor

Abhishek Chatterjee

Article

Italia et al Nat Chem Biol. 2017 Apr;13(4):446-450. d

Insert

EcWRS-h14

Expression

Bacterial

Mutation

S8A, V144G, V146C

Promoter

tacI

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-MbPylRS-4xPyOtR-ITR-GFP

Plasmid

#240124

Purpose

Express AAV2 transgene (EGFP) and MbPylRS/tRNA for ncAA incorporation

Depositor

Abhishek Chatterjee

Article

Pham et al Mol Ther Oncol. 2025 Aug 23;33(4):201040

Inserts

AAV2-ITR-EGFP
MbPylRS
PylOtR

Use

AAV

Expression

Mammalian

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pEvoltac EcW-TGA-h9

Plasmid

#218765

Purpose

encodes EcWRS-h9 and EcW-tRNA-TGA

Depositor

Abhishek Chatterjee

Article

Italia et al Nat Chem Biol. 2017 Apr;13(4):446-450. d

Insert

EcWRS-h9

Expression

Bacterial

Mutation

S8A, V144S, V146A

Promoter

tacI

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-EcYRS-4xYtR-ITR-GFP

Plasmid

#240279

Purpose

Express AAV2 transgene (EGFP) and EcYRS/tRNA for ncAA incorporation

Depositor

Abhishek Chatterjee

Article

Pham et al Angew Chem Int Ed Engl. 2026 Jan 1:e1615

Inserts

AAV2-ITR-EGFP
EcYRS
EcYtR

Use

AAV

Expression

Mammalian

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pJUMP18-pheS_NOC

Plasmid

#127031

Purpose

Basic Part NOC- Complete ORF; PheS; counter-selection marker; mutant phenylalanine tRNA synthetase.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part PheS_NOC

Use

Synthetic Biology

Expression

Bacterial

Mutation

pheS A294G promiscuous mutation

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pGEL031

Plasmid

#137900

Purpose

STU2.0 SpyCas9 tRNA system for plant genome editing

Depositor

Yong Zhang

Article

Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a-L274GMmMetRS-T2A-mCherry

Plasmid

#220800

Purpose

Lentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistance

Depositor

Elizabeth Kirby

Article

Sridharan et al Stem Cell Res Ther. 2024 Jun 26;15(1):18

Insert

Mars1 (Mars1 Mouse)

Use

Lentiviral

Tags

2xFLAG and T2A-mCherry

Mutation

mutation in MetRS to change aa 274 from L to G

Promoter

EF1a

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pRARE-MBP-DEST

Plasmid

#84650

Purpose

Gateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.

Depositor

Neil Olszewski

Article

Steiner et al Plant Cell. 2012 Jan;24(1):96-108. doi:

Type

Empty backbone

Tags

MBP

Expression

Bacterial

Promoter

tac

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAAP003l2

Plasmid

#209748

Purpose

RepA(N99D) SC101 T0-BS7 araC pBAD SD8 NpuC-C[AGGA]LFVY-NpuN-SsrA ProK 5 tRNA operon tetA-orfL bla

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

NpuC-C[AGGA]LFVY-NpuN-SsrA(rc)

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAS_4xEcoLeuT(CUA)_EF1_sfGFP150TAG

Plasmid

#174892

Purpose

amber suppression reporter sfGFP150TAG with EcoLeuT(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG in sfGFP

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGEL081

Plasmid

#137890

Purpose

iSTU-CRISPR/Cas9 with OsCDPK2 intron, tRNA structure backbone

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pTC365

Plasmid

#91214

Purpose

protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, tRNA processing)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

2 gRNAs targeting tomato ARF8A

Use

CRISPR

Expression

Plant

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCast

Plasmid

#200169

Purpose

E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme

Depositor

Yong-Su Jin

Article

Kuanyshev et al Biotechnol Bioeng. 2021 Jan;118(1):372-3

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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GLN, Linear-3, PolyU(1)

Plasmid

#166984

Purpose

Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(1)

Expression

Mammalian

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-PLRS-4xLtR-ITR-GFP

Plasmid

#240278

Purpose

Express AAV2 transgene (EGFP) and PLRS/tRNA for ncAA incorporation

Depositor

Abhishek Chatterjee

Article

Pham et al Angew Chem Int Ed Engl. 2026 Jan 1:e1615

Inserts

AAV2-ITR-EGFP
PLRS
LtR

Use

AAV

Expression

Mammalian

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-EcWRS-4xWtR-ITR-GFP

Plasmid

#240280

Purpose

Express AAV2 transgene (EGFP) and EcWRS/tRNA for ncAA incorporation

Depositor

Abhishek Chatterjee

Article

Pham et al Angew Chem Int Ed Engl. 2026 Jan 1:e1615

Inserts

AAV2-ITR-EGFP
EcWRS
EcWtR

Use

AAV

Expression

Mammalian

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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MAP80A_MS2

Plasmid

#220624

Purpose

tRNA reporter vector

Depositor

Marvin Wickens

Article

Preston et al Nat Methods. 2019 May;16(5):437-445. doi

Type

Empty backbone

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAS_4xBstTyrT(CUA)_EF1 sfGFP150TAG

Plasmid

#174891

Purpose

amber suppression reporter sfGFP150TAG expression, with BstTyr(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG in sfGFP

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pWR63

Plasmid

#203331

Purpose

episomal Cas9 and sgRNA expression vector without sgRNA for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
CaCas9
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
THD3 promoter (S. stipitis)
tRNA-sgRNA(SapI)-HDV
ADH1 terminator (S. stipitis)

Use

CRISPR

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTAG1

Plasmid

#176863

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_1

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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