165,588 results

1. gRNA-CasRx_SD1-pSV40-TagBFP

   Plasmid

   #221004

   Purpose

   Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.

   Depositor

   Mikko Taipale, Benjamin Blencowe

   Article

   Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

   Insert

   CasRx 30nt processed direct repeat with SD1 mutation
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   DR1 A>T

   Promoter

   U6

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AAV-flex-plap

   Plasmid

   #80422

   Purpose

   tracing neuronal projection

   Depositor

   Nirao Shah

   Article

   Delwig et al PLoS One. 2016 Feb 19;11(2):e0149501. do

   Insert

   plap (ALPP Human)
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRSETA_crGE

   Plasmid

   #172927

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains.

   Depositor

   Arnold Boersma

   Article

   Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

   Insert

   CrowdingSensorGE
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PP_072_pAAV_hSyn_DIO-LR-Voltron2-P2A-LR-CheRiff-HA

   Plasmid

   #230988

   Purpose

   Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

   Depositor

   Adam Cohen

   Article

   Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

   Insert

   Cre-dependent, membrane-localized optopatch for neuronal dendrites
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGD-P1/HC-Pro

   Plasmid

   #196328

   Purpose

   35S promoter-driven expression of the tobacco etch virus P1/HC-Pro RNA silencing suppressor

   Depositor

   Zhenghe Li

   Article

   Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

   Insert

   tobacco etch virus P1/HC-Pro
   

   Expression

   Plant

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PGKp-GFP-YAP (5SA)

   Plasmid

   #174174

   Purpose

   Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the human PGK promoter.

   Depositor

   Rod Bremner

   Article

   Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

   Insert

   YAP (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   3x FLAG tag

   Expression

   Mammalian

   Mutation

   Five LATS phosphorylation sites (S61, S109, S127,…

   Promoter

   PGK

   Available Since

   Aug. 19, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

   Plasmid

   #183410

   Purpose

   piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells

   Depositor

   Azim Surani

   Article

   Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

   Insert

   KRAB-dCas9-ecDHFR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1

   Plasmid

   #118593

   Purpose

   Expresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoter

   Depositor

   Stanley Qi

   Article

   Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

   Insert

   Puro-T2A-Tet-on 3G/Ascl1 (Ascl1 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha/TRE3G

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDH-EF1a-eFFly-eGFP

   Plasmid

   #104834

   Purpose

   eFFly luciferase for invivo Imaging, eGFP for flourescent detection

   Depositor

   Irmela Jeremias

   Article

   Sandhofer et al Leukemia. 2015 Apr;29(4):828-38. doi: 10

   Inserts

   eFFly
   eGFP
   

   Use

   Lentiviral and Luciferase; Egfp

   Expression

   Mammalian

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1/Puro-CAG-ASAP2s

    Plasmid

    #101274

    Purpose

    Genetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)

    Depositor

    Francois St-Pierre

    Article

    Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

    Insert

    ASAP2s
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBMN mEGFP-OPTN

    Plasmid

    #188784

    Purpose

    For mammalian expression of OPTN tagged with mEGFP

    Depositor

    Michael Lazarou

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    OPTN (OPTN Human)
    

    Use

    Retroviral

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAV-∆Hapln1

    Plasmid

    #200778

    Purpose

    Expresses mouse HAPLN1 mutant lacking the lectican binding domain and fused with cysteine-free GFP (cfGFP) under synapsin promoter

    Depositor

    Alexander Dityatev

    Article

    Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

    Insert

    Hyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
    

    Use

    AAV

    Tags

    cfGFP

    Mutation

    deleted lectican binding domain (aminoacids 40-15…

    Promoter

    Synapsin

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFX-Tom20-ｍCherry

    Plasmid

    #174188

    Purpose

    Express mCherry with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    mCherry with mitchondria-targeting sequence
    

    Tags

    the mitochondria-targeting sequence of TOM20

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAG1186 Lyn11-ECFP-FIREmate

    Plasmid

    #205168

    Purpose

    Expresses Lyn11-ECFP-FIREmate in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    Lyn11-ECFP-FIREmate
    

    Expression

    Mammalian

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. UbcH5c WT pET28a

    Plasmid

    #12643

    Depositor

    Rachel Klevit

    Article

    Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

    Insert

    UbcH5c - Ubiquitin conjugating enzyme (UBE2D3 Human)
    

    Expression

    Bacterial

    Available Since

    July 31, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GAPDH-CPG-TV

    Plasmid

    #70148

    Purpose

    a targeting vector, to target the endogenous Gapdh CPG island with Snrp-GFP-polyA

    Depositor

    Rudolf Jaenisch

    Article

    Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

    Insert

    5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh
    

    Use

    Mouse Targeting and Synthetic Biology

    Available Since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pArcCreER

    Plasmid

    #46389

    Depositor

    Liqun Luo

    Article

    Guenthner et al Neuron. 2013 Jun 5;78(5):773-84. doi: 10

    Inserts

    Arc (Arc Mouse)
    CreERT2
    

    Use

    Mouse Targeting

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCap-SG5-Apr

    Plasmid

    #227579

    Purpose

    Capture plasmid with apramycin resistance and pSG5 replicon

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Type

    Empty backbone

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA3.1(+) miniTurboID-6xGGSG linker-FKBPF36V-2xHA

    Plasmid

    #200641

    Purpose

    Expresses miniTurboID-FKBPF36V-2xHA in mammalian cells.

    Depositor

    Justin English, Fleur Ferguson

    Article

    Tao et al Nat Commun. 2023 Dec 4;14(1):8016. doi:

    Insert

    miniTurboID-6xGGSG linker-FKBPF36V-2xHA
    

    Tags

    HA-tag

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBS-L1PA1CH_blast rescue

    Plasmid

    #69611

    Purpose

    Expresses a blasticidin tagged codon optimized L1PA1 designed to recover inserts

    Depositor

    Astrid Roy-Engel

    Insert

    L1PA1
    

    Tags

    blast rescue cassette

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge