174,397 results

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FLAG.PKCzeta.T410E

Plasmid

#10804

Depositor

Alex Toker

Article

Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

Insert

PKC zeta (Prkcz Rat)

Tags

FLAG

Expression

Mammalian

Mutation

Activation loop active: T410E (Thr 410 to Glu)

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
1053 pBabe puroL Akt K179M T308A S473A

Plasmid

#9013

Depositor

William Sellers

Article

DiRenzo et al Cancer Res. 2002 Jan 1. 62(1):89-98.

Insert

Akt1 K179M T308A S473A (AKT1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

K179M T308A S473A

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pPS2991

Plasmid

#8931

Depositor

Pamela Silver

Article

Devroe et al J Biol Chem. 2004 Jun 4. 279(23):24444-5

Insert

NDR2 (STK38L Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
6xHis-SNAP-PI(3)P Probe

Plasmid

#211508

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteria

Depositor

Hannes Maib, David Murray

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

FYVE-Hrs x 2 (PIKFYVE Human)

Tags

6xhis-SNAP

Expression

Bacterial

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLX304_CMV::mCherry-LgBiT-FLAG-NES

Plasmid

#199715

Purpose

Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

Depositor

Alice Ting

Article

Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

Insert

mCherry-LgBiT-FLAG-NES

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pS-sg:GFP

Plasmid

#196296

Purpose

35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSs

Depositor

Zhenghe Li

Article

Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

Insert

Full length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs

Use

CRISPR

Expression

Plant

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_NTD-Cre

Plasmid

#198274

Purpose

For loading Cre into PVCs; also contains PVC regulatory genes

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-Cre

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pmScarlet3-Giantin_C1

Plasmid

#189773

Purpose

Construct for labelling Golgi apparatus in mammalian cells using mScarlet3-Giantin fusion

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3-Giantin

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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psfGFP-LAMP1-mCherry (pHLARE)

Plasmid

#164477

Purpose

Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.

Depositor

Diane Barber

Article

Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

Insert

LAMP1 (Lamp1 Rat)

Tags

mCherry and superfolder GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis-Taq DNA Ligase

Plasmid

#178798

Purpose

Inducible expression of Taq DNA Ligase in E. coli

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

Taq DNA Ligase

Expression

Bacterial

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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Gi2-CASE

Plasmid

#168121

Purpose

Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi2. Composed of the subunits G alpha i2 (GNAI2) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

Depositor

Gunnar Schulte

Article

Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

Insert

GNAI2, GNB1, GNG2 (GNAI2, GNB1, GNG2 Human)

Use

Luciferase

Tags

cpVenus on GNG2

Expression

Mammalian

Mutation

NLuc is inserted at C112/E115 within GNAI2

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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G13-CASE

Plasmid

#168127

Purpose

Encodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

Depositor

Gunnar Schulte

Article

Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

Insert

GNA13, GNB3, GNG9 (GNA13, GNB3, GNGT2 Human)

Use

Luciferase

Tags

cpVenus on GNG9

Expression

Mammalian

Mutation

NLuc is inserted at F125/D126 within GNA13

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Soma-jGCaMP8m

Plasmid

#169257

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

Soma-jGCaMP8m

Use

AAV

Tags

6xHis-tag

Expression

Mammalian

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pRH030

Plasmid

#159633

Purpose

CRISPR‐Cas9 tool for the genetic engineering of Bacillus phages

Depositor

Robert Hertel

Article

Otte et al Lett Appl Microbiol. 2020 Jul 2. doi: 10

Type

Empty backbone

Use

CRISPR; Shuttle vector

Promoter

Constitutive tracrRNA

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_eCB2.0

Plasmid

#164604

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in neurons

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid sensor GRAB_eCB2.0

Use

AAV

Mutation

S383T

Promoter

hSyn

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMK265 (RAD21-mAC Hygro)

Plasmid

#140539

Purpose

RAD21 tagging with mAID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

RAD21 (RAD21 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-NLS-dPguCas13b-3xmRuby3-NLS

Plasmid

#132406

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPguCas13b

Use

Lentiviral

Expression

Mammalian

Mutation

H151A; H1121A

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-mito-Citron1

Plasmid

#134305

Purpose

mitochondria-targeted direct-response citrate sensor

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citron1

Expression

Mammalian

Promoter

CMV

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pTol2-HuC(elavl3)-CaMPARI2

Plasmid

#137185

Purpose

pan-neuronal expression of CaMPARI2 in zebrafish, used to generate the CaMPARI2 zebrafish line at ZIRC (ZL13801)

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Insert

HuC(elavl3)-CaMPARI2-FLAG-HA-myc-polyA

Use

Tol2 plasmid for zebrafish

Tags

FLAG, HA, myc

Promoter

HuC(elavl3)

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-EGFP-NLS-3xFlag

Plasmid

#132397

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1058A

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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