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Showing: 9061 - 9080 of 66816 results

  2. pEF1a_HH-EMX1 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

    Plasmid
    #176459
    Purpose
    Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting EMX1 driven by EF1alpha promoter and and EGFP(L138S)-P2A-mCherry driven by CMV
    Depositor
    Hui Yang
    Article
    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/
    Insert
    HH-EMX1 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1alpha
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    176459-plasmid-map-sequence-id-346304

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  4. Exp-pcDNA3.2delCMV(EF1α-tTA/TetO-mCh-Rs1)

    Plasmid
    #26797
    Purpose
    Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Construct expresses both mCherry and Rs1 via a P2A ribosomal skip sequence. Expresses tTA under EF1α promoter separately.
    Depositor
    Edward Hsiao
    Article
    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
    Inserts
    Rs1 (HTR4 Human)
    tTA
    Tags
    mCherry-P2A
    Expression
    Mammalian
    Mutation
    D100A
    Promoter
    EF1α and short TetO, miniCMV
    Available Since
    Feb. 23, 2011
    Availability
    Academic Institutions and Nonprofits only
    26797-plasmid-map-sequence-id-12685

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  6. pEMS1062

    Plasmid
    #29011
    Purpose
    The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.
    Depositor
    Elizabeth Simpson
    Article
    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414
    Insert
    Ple155-Cre/EGFP-NLS
    Use
    High copy number homologous recombination plasmid…
    Expression
    Mammalian
    Promoter
    MiniPromoter Ple155 from the human PCP2 gene
    Available Since
    Feb. 22, 2012
    Availability
    Academic Institutions and Nonprofits only
    29011-plasmid-map-sequence-id-13417

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  13. pCIBN-LacI-tagRFP-T

    Plasmid
    #103810
    Purpose
    'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-LacI-tagRFP-T (CIB1, lacI Mustard Weed, E. coli)
    Expression
    Mammalian
    Mutation
    LacI: C19T (silent, L7L), T264C (silent, A88A), C…
    Promoter
    CMV
    Available Since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103810-plasmid-map-sequence-id-199266

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Showing: 9061 - 9080 of 66816 results