We narrowed to 9,694 results for: pho
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Plasmid#203606PurposeReporter plasmid carrying PHLPP2 3'UTR with mutated miR-27b target sequencesDepositorInsertPH domain and leucine rich repeat protein phosphatase 2 (PHLPP2 Human)
UseLuciferaseExpressionMammalianMutationmiR-27b target sequences in the 3'UTR were m…PromoterSV40Available SinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Sema6a.a-Fc-His
Plasmid#72163PurposeExpresses the extracellular region of the Sema6A, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CaMPARI W391F V398L
Plasmid#60787PurposeExpresses CaMPARI W391F V398L in mammalian cells, a photoconvertible fluorescent protein-based calcium integratorDepositorInsertCaMPARI W391F V398L
TagsNuclear Export SignalExpressionMammalianPromoterCMVAvailable SinceNov. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
His-DNPH1
Plasmid#203224PurposeFor protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-INPP4B
Plasmid#116864PurposeRecruitable PI(3,4)P2 4-phosphataseDepositorAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-DIO-mCReP
Plasmid#167503PurposeConditional expression of the eIF2alpha phophatase, CReP (Ppp1r15b)DepositorAvailable SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
p6608 MSCV-IP N-HAonly CHAF1B
Plasmid#34901DepositorAvailable SinceFeb. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)
Plasmid#195741PurposeBase editing for making a pre-stop codon in mmoX using phenol inducible systemDepositorArticleInsertDi-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPo (phoenol-inducible promoter)Available SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
SiriusGFP
Plasmid#123201PurposeWe have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.DepositorInsertSiriusGFP
UseMouse TargetingTagsN/AExpressionBacterial and MammalianMutationeGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…PromoterCMVAvailable SinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGLACTE-PseudoHT51
Plasmid#99257PurposeContains expanded CGG repeats (98 repeats with 2 AGG interruptions) within the 5'UTR of FMR1. Internal control with synthetic primer sequence for capillary electrophoresisDepositorInsertFMR1 (FMR1 Human, Synthetic)
MutationContains synthetic, non-human, primer sequence do…Available SinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-INPP4B-CAAX
Plasmid#116857Purposeplasma membrane-targetted PI(3,4)P2 4-phosphataseDepositorAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
VRK1_HUMAN_D0
Plasmid#79684PurposeThis plasmid encodes the kinase domain of VRK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
Plxna1-AP-His
Plasmid#71996PurposeExpresses the extracellular region of the PlexinA1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
L2096-GPA-BRET-Biosensor-Retrovirus-TD146
Plasmid#222878PurposeRetroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with BRET (split NanoLuciferase reconstitution [11S/114 fragment]). WT Glycophorin A (GPA) scaffold.DepositorInsertFRB-GPA-NanoLuc114-CyOFP1-T2A-FKBP-GPA-NanoLuc11S
UseRetroviralTagsMycExpressionMammalianPromoterMSCV LTRAvailable SinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry1-Mito-7
Plasmid#57189PurposeLocalization: Mitochondria, Excitation: 400 / 504, Emission: 515 / 517DepositorAvailable SinceJan. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-INPP4BC842A
Plasmid#116865PurposeInactive recruitable PI(3,4)P2 4-phosphataseDepositorInsertINPP4B (INPP4B Human)
TagsFKBP12 and mCherryExpressionMammalianMutationcatalytic cysteine 842 changed to alanineAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Sema7a-Fc-His
Plasmid#72175PurposeExpresses the extracellular region of the Sema7A protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Perox-mTurq2-Apollo-NADP+
Plasmid#193304PurposeExpresses mTurq2-Apollo-NADP+ sensor localized to the peroxisomeDepositorInsertG6PD (G6PD Human)
TagsmTurqoise2, peroxisomal targeting sequenceExpressionMammalianMutationS40A, R72Q, H201N, K205TPromoterT7Available SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2R3a-3AT
Plasmid#71273PurposeEntry clone containing 3AT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertT3A ribulose-1,5-bisphosphate carboxylase 3A subunit terminator
UseGatewayAvailable SinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
FAK2_HUMAN_D0
Plasmid#79747PurposeThis plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only