59,428 results

1. H52-pUCM-hNGN2-inv

   Plasmid

   #110492

   Purpose

   Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology arm

   Depositor

   Michael Ward

   Inserts

   Neurogenin 2 (NEUROG2 Human)
   mcherry
   rtTA3G
   

   Use

   CRISPR, Cre/Lox, and TALEN

   Expression

   Mammalian

   Promoter

   CAG, EF-1 alpha, and TRE3G

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5-myc-Rac1-T17N

   Plasmid

   #12984

   Depositor

   Gary Bokoch

   Insert

   Rac1 DN (RAC1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   T17N Dominant negative

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_CHD2_WT

   Plasmid

   #81768

   Purpose

   Gateway Donor vector containing CHD2 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   CHD2 (CHD2 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. H2B-mCherry

   Plasmid

   #20972

   Purpose

   H2B fused to mCherry for chromatin visualization

   Depositor

   Robert Benezra

   Article

   Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

   Insert

   Histone cluster 1, H2bb (H2BC3 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   May 7, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIRES2-DsRed-MST-Kv2.1

   Plasmid

   #131707

   Purpose

   Expresses Kv2.1in mammalian cells

   Depositor

   Jennifer Kearney

   Article

   Jorge et al Proc Natl Acad Sci U S A. 2011 Mar 29;10

   Insert

   KCNB1 (KCNB1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. psd44-G13WT

   Plasmid

   #46829

   Purpose

   expression of wild type G protein alpha 13 in mammalian cells

   Depositor

   Agnese Mariotti

   Insert

   GNA13 (GNA13 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   PGK promoter

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pT7-EGFP-C1-HsDCP2-E148Q_U

   Plasmid

   #147650

   Purpose

   Mammalian Expression of HsDCP2-E148Q

   Depositor

   Elisa Izaurralde

   Article

   Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

   Insert

   HsDCP2-E148Q (DCP2 Human)
   

   Expression

   Mammalian

   Mutation

   two silent mutations compared to the sequence giv…

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. GOLPH3 shRNA

   Plasmid

   #163860

   Purpose

   shRNA targeting the coding sequences of GOLPH3

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   golgi phosphoprotein 3 (GOLPH3 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a(CTLA4)

   Plasmid

   #177905

   Purpose

   Bacterial expression of his10-tagged human CTLA4 Intracellular Domain

   Depositor

   Ron Vale

   Article

   Hui et al Science. 2017 Mar 31;355(6332):1428-1433

   Insert

   CTLA4 (aa 183-223 only) (CTLA4 Human)
   

   Tags

   10xHis-Thrombin cut site-T7 tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. GalT-RpHLuorin2

    Plasmid

    #171719

    Purpose

    Trans-Golgi expression of ratiometric pHLuorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 fused to B4GALT1 (B4GALT1 Human)
    

    Tags

    ratiometric pHluorin2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7-EGFP-C1-HsNot7-D40AE42A_L

    Plasmid

    #146890

    Purpose

    Mammalian Expression of HsNot7-D40AE42A. Please note that this plasmid does not contain the T7 promoter.

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    HsNot7-D40AE42A (CNOT7 Human)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. LPAR4-DuET

    Plasmid

    #213331

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    LPAR4 (LPAR4 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLNHA-C1-HsLSm1_J

    Plasmid

    #146658

    Purpose

    Mammalian Expression of HsLSm1

    Depositor

    Elisa Izaurralde

    Article

    Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

    Insert

    HsLSm1 (LSM1 Human)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pD649-HAsp-CTLA4-COMP5AP-AviTag-9xHis

    Plasmid

    #157074

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    CTLA4 (CTLA4 Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIG-719 CTLA4-GFP R70W

    Plasmid

    #186124

    Purpose

    CTLA4 gene knockin with mutation

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CTLA4 (CTLA4 Human)
    

    Use

    CRISPR

    Mutation

    R70W

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. hKCNB1 L211P

    Plasmid

    #124487

    Purpose

    missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

    Depositor

    Federico Sesti

    Article

    Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

    Insert

    human KCNB1 (KCNB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed leucine 211 to proline

    Available Since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. hKCNB1 G381R

    Plasmid

    #124490

    Purpose

    missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

    Depositor

    Federico Sesti

    Article

    Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

    Insert

    human KCNB1 (KCNB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed glycine at 381 to arginine

    Available Since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. hKCNB1 F416L

    Plasmid

    #124491

    Purpose

    missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

    Depositor

    Federico Sesti

    Article

    Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

    Insert

    human KCNB1 (KCNB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed phenyalanine 416 to leucine

    Available Since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. hC73M-HA

    Plasmid

    #111528

    Purpose

    Expresses human KCNB1 C73M in mammalian cells

    Depositor

    Federico Sesti

    Insert

    human C73M-Kv2.1 + HA (KCNB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C73M

    Promoter

    CMV

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223_CHD2_p.P195L

    Plasmid

    #81463

    Purpose

    Gateway Donor vector containing CHD2 ,part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    CHD2 (CHD2 Human)
    

    Use

    Gateway entry vector

    Mutation

    P195L

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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