We narrowed to 1,197 results for: Aspa

1. pMEH343 CD63 MS2 HA

   Plasmid

   #168217

   Purpose

   Mammalian expression of tetraspanin CD63 fused to the MS2 dimer and C-terminal HA tag

   Depositor

   Joshua Leonard

   Article

   Hung et al J Extracell Vesicles. 2016 May 13;5:3102

   Insert

   CD63 (CD63 Human)
   

   Use

   Tags

   HA and MS2 dimer

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs-Stat3-C

   Plasmid

   #13373

   Purpose

   Retroviral expression of dominant active mouse Stat3

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   signal transducer and activator of transcription 3 (dominant active mutant) (Stat3 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed Alanine 661 to cysteine and asparagine 66…

   Promoter

   Available since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-FLAG-Rheb-N153T

   Plasmid

   #19997

   Depositor

   Fuyuhiko Tamanoi

   Article

   Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

   Insert

   Ras homolog enriched in brain 1 - N153T (RHEB Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Changed Asparagine 153 to Threonine

   Promoter

   Available since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pRN3P-NLS-EGFP-deadBtgh-3NLS

   Plasmid

   #194470

   Purpose

   Encodes for catalytically inactive bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.

   Depositor

   Mathieu Boulard

   Article

   Formichetti et al Dev Cell 10 Jan 2024

   Insert

   BtGH84
   

   Use

   For in vitro transcription from t3 promoter after…

   Tags

   SV40 Nuclear Localization Signal, SV40 Nuclear Lo…

   Expression

   Mutation

   Aspartic Acid 242 (in Dennis et al.) changed to A…

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-20b-M-Ntx-S1

   Plasmid

   #58716

   Purpose

   Expresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1S

   Depositor

   Fiorella Tonello

   Article

   Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

   Insert

   Notexin
   

   Use

   Tags

   Methionine

   Expression

   Bacterial

   Mutation

   Changed Asparagine 1 to Serine

   Promoter

   T7

   Available since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. YFP-CD82

   Plasmid

   #1819

   Depositor

   Walther Mothes

   Article

   Sherer et al Traffic 2003 Nov;4(11):785-801.

   Insert

   CD82 (CD82 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-NT-3/D15A

   Plasmid

   #73035

   Purpose

   Express an NT-3-BDNF hybrid neurotrophic factor in mammalian cells

   Depositor

   Pantelis Tsoulfas

   Article

   Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

   Insert

   NT-3/D15A (NTF3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Mutated Aspartic acid in position 15 of the matur…

   Promoter

   Available since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 myc card9

   Plasmid

   #16253

   Depositor

   William Kaelin

   Article

   Yang et al Mol Cell. 2007 Oct 12. 28(1):15-27.

   Insert

   card9 (CARD9 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. Opto-casp9_AtCRY2-PHR-casp9

   Plasmid

   #131221

   Purpose

   Expresses Opto-casp9_AtCRY2-PHR-casp9 (light-activated caspase9)

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   Opto-casp9 (AtCRY2-PHR-casp9)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-Foxo1ADA(pCMV5)

    Plasmid

    #12149

    Depositor

    Domenico Accili

    Article

    Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

    Insert

    Foxo1 ADA (Foxo1 Mouse)
    

    Use

    Tags

    Flag and Myc

    Expression

    Mammalian

    Mutation

    Threonine 24 to Alanine (A) and Serine 253 to Asp…

    Promoter

    CMV

    Available since

    June 13, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pRK5 TGF beta type I receptor (T202D) Flag

    Plasmid

    #14833

    Depositor

    Rik Derynck

    Article

    Feng et al J Biol Chem. 1996 May 31. 271(22):13123-

    Insert

    TGF beta type I receptor (Tgfbr1 Rat)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Threonine 202 mutated to aspartate. Constitutivel…

    Promoter

    Available since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3/hArf5(T31N)-HA

    Plasmid

    #79429

    Purpose

    Expresses C-terminally HA-tagged Arf5(T31N) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Hanai et al J Biochem. 2016 Feb;159(2):201-8. doi: 1

    Insert

    Arf5 (ARF5 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Threonine 31 to Asparagine

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry

    Plasmid

    #124434

    Purpose

    Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Casp3 cleavage seq) T2A mCherry
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. mTagBFP2-CD151-7

    Plasmid

    #55282

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    CD151 (CD151 Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

15. CFP-CD82

    Plasmid

    #1818

    Depositor

    Walther Mothes

    Article

    Sherer et al Traffic 2003 Nov;4(11):785-801.

    Insert

    CD82 (CD82 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti6-BMPR1A(Q233D)-V5

    Plasmid

    #35638

    Depositor

    Daniel Haber

    Article

    Singh et al Cell. 2012 Feb 17;148(4):639-50.

    Insert

    BMP Receptor Type1A Q233D (BMPR1A Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Glutamine 233 to Aspartate

    Promoter

    CMV

    Available since

    April 26, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pCINeoFlag ALIX; I212D

    Plasmid

    #89861

    Purpose

    Expresses full length Flag-tagged ALIX with mutation I212D in mammalian cells; internal ID WISP05-117

    Depositor

    Wesley Sundquist

    Article

    Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

    Insert

    ALIX (PDCD6IP Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Mutated isoleucine 212 to aspartic acid

    Promoter

    CMV

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAH-CTX1-rhadCas9-native

    Plasmid

    #129392

    Purpose

    Derived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Aspartate 10 to Alanine, Histidine 840 to Alanine

    Promoter

    PrhaBAD

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. GA-DEVD-GB-NES

    Plasmid

    #61020

    Purpose

    green ddFP based caspase-3 biosensor

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP A and ddGFP B
    

    Use

    Tags

    HindIII and NES sequence LALKLAGLDIGS placed afte…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-RIPK3[S227D]-FLAG

    Plasmid

    #199320

    Purpose

    mutated phosphorylation site (MLKL interaction permanent active)

    Depositor

    Adrian Press

    Article

    Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

    Insert

    Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 227 to Aspartic Acid

    Promoter

    Available since

    May 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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