We narrowed to 3,091 results for: sam

1. MTK234_029

   Plasmid

   #123909

   Purpose

   Encodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   sgRNA3 -hThal5.10-2_hg18
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. MTK6_013

   Plasmid

   #123867

   Purpose

   Encodes Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) expressing a Blasticidin resistance cassette without a terminator to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   WPRE-Blast (no terminator)
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. MTK3_018

   Plasmid

   #123733

   Purpose

   Encodes rapamycin and tamoxifen activated split-dCAS9 (N-terminal) as a Type 3 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   spdCas9(1-204)-frb-D10A-ERT2
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHES 946

   Plasmid

   #112644

   Purpose

   blue light inducible cryptochrome based optogenetic expression system

   Depositor

   Hana El-Samad

   Article

   Harrigan et al Cell. 2018 Oct 18;175(3):877-886.e10. do

   Insert

   RTG3AD-GSx7-CIB1-SV40NLS (CIB1 Budding Yeast, Synthetic, Mustard Weed)
   

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW57.1-MAT2A

   Plasmid

   #100521

   Purpose

   Dox-repressible cDNA expression of MAT2A (dox-off/ tet-off all-in-one vector)

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   MAT2A (MAT2A Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   pTight TRE

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. PIK3CA - Exon20 H1047R

   Plasmid

   #16639

   Depositor

   Bert Vogelstein

   Article

   Samuels et al Science. 2004 Apr 23. 304(5670):554.

   Insert

   PIK3CA H1047R (PIK3CA Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   H1047R

   Available Since

   March 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMax_mOrange_G299C

   Plasmid

   #177829

   Purpose

   Site-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR).

   Depositor

   Leona Samson, Zachary Nagel

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   mOrange_G299C
   

   Expression

   Mammalian

   Mutation

   G299C

   Promoter

   CMV

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. BCAM promoter-pRB-Puro-TurboRFPmyc-T2A-Luciferase

   Plasmid

   #188391

   Purpose

   Reporter for BCAM expression

   Depositor

   Markus Frank

   Article

   Sasamoto et al Cell Rep. 2022 Aug 9;40(6):111166. doi:

   Insert

   BCAM reporter
   

   Use

   Luciferase

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMax_mOrange_A215C

   Plasmid

   #177828

   Purpose

   Site-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).

   Depositor

   Leona Samson, Zachary Nagel

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   mOrange_A215C
   

   Expression

   Mammalian

   Mutation

   A215C

   Promoter

   CMV

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. EF1a-LCB1_v1.3-PDGFR-T2A-mCherry (pZYW048)

    Plasmid

    #194231

    Purpose

    Lentiviral expression vector expressing LCB1 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    EF1a-LCB1-PDGFR TMD-T2A-mCherry
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMax_GFP_C289T

    Plasmid

    #177826

    Purpose

    Site-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR).

    Depositor

    Leona Samson, Zachary Nagel

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    GFP_C289T
    

    Expression

    Mammalian

    Mutation

    C289T

    Promoter

    CMV

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMax_BFP_A191G

    Plasmid

    #177823

    Purpose

    Site-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).

    Depositor

    Leona Samson, Zachary Nagel

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    tagBFP_A191G
    

    Expression

    Mammalian

    Mutation

    A191G

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. CD40L-MIEG-hCD4 (hCD4-CD40L)

    Plasmid

    #40355

    Depositor

    Alexander Dent

    Article

    Kusam et al Int J Cancer. 2009 Aug 15;125(4):977-81.

    Insert

    CD40L (Cd40lg Mouse)
    

    Use

    Retroviral

    Tags

    IRES hCD4

    Available Since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

14. SynNotch TMD/Gal4-VP64 ICD (MTK 3b) (pAN1031)

    Plasmid

    #194222

    Purpose

    Mammalian Toolkit part 3b encoding SynNotch and GAL4-VP64 to be used in designing modular receptors

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    SynNotch-GAL4 DBD-VP64 AD
    

    Use

    Synthetic Biology

    Promoter

    None

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-ERBB4 (kinase dead)

    Plasmid

    #29533

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

    Insert

    ERBB4 (ERBB4 Human)
    

    Expression

    Mammalian

    Mutation

    changed lysine 751 to methionine

    Available Since

    July 20, 2011

    Availability

    Academic Institutions and Nonprofits only

16. OmEF1aCas9P2ApuroSB

    Plasmid

    #165484

    Purpose

    Stable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cells

    Depositor

    Dietmar Kueltz

    Article

    Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10

    Insert

    Zebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)
    

    Use

    CRISPR; Transposon

    Promoter

    Oreochromis mossambicus EF1 alpha promoter (OmEF1…

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDF1-ERBB4 (kinase dead)

    Plasmid

    #29544

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

    Insert

    ERBB4 (ERBB4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    changed lysine 751 to methionine

    Available Since

    July 20, 2011

    Availability

    Academic Institutions and Nonprofits only

18. JPF0419d

    Plasmid

    #124020

    Purpose

    Encodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pCAG-Lyn-iRFP713-P2A-NES-mAzamiGreen-P2A-p38KTR-mCerulean-P2A-H2B::mScarlet
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PIK3CA - Exon9 E545K (pFastBac)

    Plasmid

    #16640

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Science. 2004 Apr 23. 304(5670):554.

    Insert

    PIK3CA E545K (PIK3CA Human)
    

    Use

    Baculoviral

    Tags

    His

    Expression

    Mammalian

    Mutation

    E545K

    Available Since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

20. PIK3CA - Exon20 H1047R (pFastBac)

    Plasmid

    #16641

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Science. 2004 Apr 23. 304(5670):554.

    Insert

    PIK3CA H1047R (PIK3CA Human)
    

    Use

    Baculoviral

    Tags

    His

    Expression

    Mammalian

    Mutation

    H1047R

    Available Since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only