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pME_Cp_0_5_020_psaC_3'UTR_Cr

Plasmid

#235917

Purpose

psaC 3'UTR Chlamydomonas reinhardtii

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

psaC 3'UTR

Use

Synthetic Biology

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_3_015_psbC_5'UTR_Cr

Plasmid

#235842

Purpose

psbC 5'UTR Chlamydomonas reinhardtii

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

psbC 5'UTR

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_3_001_5'UTR_placeholder

Plasmid

#235830

Purpose

Encodes a sfGFP cassette in the 5'UTR position used for placeholder cloning

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5'UTR placeholder

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_3_032_rbcL_5'UTR_full_Nt

Plasmid

#235851

Purpose

rbcL 5'UTR full Nicotiana tabacum

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

rbcL full 5'UTR Nt

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCK761

Plasmid

#242916

Purpose

Constitutive expression of dCas9, αNTD-MCP, and PspF-λN22

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

PspF-λN22 (pspF Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCK948

Plasmid

#242917

Purpose

Constitutive expression of dCas9, MCP-TetD, and PspF-λN22

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

PspF-λN22 (pspF Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pKO025

Plasmid

#242913

Purpose

aTc-inducible expression of Spy-dCas9-AsiA and Sth1-dCas9, and constitutive expression of MCP-SoxS

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

Sth1-dCas9

Use

CRISPR and Synthetic Biology

Promoter

Ptet

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCK660

Plasmid

#242914

Purpose

Constitutive expression of dCas9, MCP-SoxS, and PspF-λN22

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

PspF-λN22 (pspF Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCK320

Plasmid

#242906

Purpose

aTc-inducible expression of dCas9-AsiA

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

AsiA (asiA Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

TetR-Ptet

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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DoS3 plasmid

Plasmid

#244471

Purpose

Containing RP4 oriT and Cas9 module, for eliminating RP4 plasmid.

Depositor

Lingchong You

Article

Tsoi et al Sci Adv. 2025 Apr 4;11(14):eads4735. doi

Insert

I-SceI enzyme (CSS3 Synthetic)

Use

Synthetic Biology

Promoter

araBAD promoter

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pBD-PYR1-MANDI

Plasmid

#241279

Purpose

Yeast two hybrid vector expressing BD-PYR1-MANDI

Depositor

Sean Cutler

Article

Park et al Nature. 2015 Apr 23;520(7548):545-8. doi

Insert

PYR1_mandi (PYR1 Mustard Weed)

Expression

Yeast

Mutation

NA

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-BacD-23005

Plasmid

#109185

Purpose

Plasmid donor for expression of proteins under the control of a minimal HSP70 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Article

Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.

Type

Empty backbone

Expression

Insect

Promoter

minimal HSP70 promoter

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pEvol-chKlacRS-WT

Plasmid

#232159

Purpose

A chimeric PylRS for site-specific incorporation of L-lactyl-lysine into target proteins in E.coli cells.

Depositor

Nanxi Wang

Article

Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

Insert

chKlacRS-WT

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

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NanoBRET Assay Vector, NanoLuc-NRAS(Q61K)

Plasmid

#236854

Purpose

Express NanoLuc(R)-NRAS(Q61K) in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

NRAS (NRAS Human)

Tags

HaloTag (R) and NanoLuc (R)

Expression

Mammalian

Mutation

Q61K

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pMTKM22

Plasmid

#233494

Purpose

Level 0 plasmid for MoClo, encodes the INU1 promoter for K.marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

promINU1

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM23

Plasmid

#233495

Purpose

Level 0 plasmid for MoClo, encodes the ADH1 promoter for K.marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

promADH1

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM24

Plasmid

#233496

Purpose

Level 0 plasmid for MoClo, encodes the PGK promoter for K.marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

promPGK1

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM25

Plasmid

#233497

Purpose

Level 0 plasmid for MoClo, encodes the TEF3 promoter for K.marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

promTEF3

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM26

Plasmid

#233498

Purpose

Level 0 plasmid for MoClo, encodes the TDH3 promoter for K.marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

promTDH3

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM27

Plasmid

#233499

Purpose

Level 0 plasmid for MoClo, encodes the HFF1 promoter for K.marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

promHFF1

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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