We narrowed to 23,470 results for: SCR

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pKLV2-EF1aMS2p65HSF1hyg-W

Plasmid

#112920

Purpose

Lentiviral vector expressing MS2p65HSF1 under the control of the long human EF1a promoter

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

MS2p65HSF1-T2Ahyg

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human EF1a promoter

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET_His6-MBP-TEV-Cas2

Plasmid

#186275

Purpose

Expresses Thiomicrospira Cas2 in E. coli

Depositor

Jennifer Doudna

Article

Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

Insert

Thiomicropira Cas2

Tags

His6-MBP

Expression

Bacterial

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pJZC572

Plasmid

#62318

Purpose

sgRNA with 1x com for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 1x com RNA binding module

Expression

Yeast

Promoter

SNR52

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pJet1.2 Tev-Halo tag

Plasmid

#117124

Purpose

C-terminal Tev Halo-Tag

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Tev-Halo-tag Tag

Use

Golden gate donor vector for gene targeting in ba…

Tags

Tev-Halo-Tag

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-SLBP

Plasmid

#45082

Depositor

Phillip Newmark

Article

Rouhana et al Development. 2014 Jul;141(13):2592-601.

Insert

Stem Loop Binding Protein

Use

RNAi; In vitro transcription

Promoter

T7 sense, T3 antisense

Available Since

May 29, 2013

Availability

Academic Institutions and Nonprofits only
pEPYCeGM0001

Plasmid

#196141

Purpose

Gateway entry vector with Arabidopsis TGA1 CDS and c-terminal HiBiT tag

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtTGA1_HiBit

Use

Gateway entry vector

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsAGO3_F

Plasmid

#146316

Purpose

Mammalian Expression of HsAGO3

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsAGO3 (AGO3 Human)

Expression

Mammalian

Mutation

T25A

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ141C2.0

Plasmid

#99905

Purpose

Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

OsU6

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pVW284

Plasmid

#139494

Purpose

Expression vector for the PP7-GFP protein in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pADH1 PP7∆FG-GFPenvy tCYC1

Expression

Yeast

Mutation

∆FG

Promoter

pADH1

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pVW300

Plasmid

#139495

Purpose

Expression vector for the PP7-GFP protein in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pTEF PP7∆FG-GFPenvy tCYC1

Expression

Yeast

Mutation

∆FG

Promoter

pTEF

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pBzCas13b

Plasmid

#164862

Purpose

Inducible expression of BzCas13b nuclease in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

Bergeyella zoohelcum Cas13b

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF4D09

Plasmid

#70941

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

MED25 (MED25 Fly)

Use

Gateway entry vector

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
env8 selectivity (d')

Plasmid

#99838

Purpose

mNeonGreen reporter that places expression of a fluorescent protein reporter (mNeonGreen) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al J Biol Chem. 2017 May 8. pii: jbc.M117.7

Insert

env8 selectivity (d')

Tags

mNeonGreen

Expression

Bacterial

Mutation

A20G, A68G, G-C pairs in P1 and P3, G12A, G10U de…

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCsy-crRNA-EV

Plasmid

#153944

Purpose

For cloning and expression of Type I-F P. aeruginosa (PaeCascade) crRNA in mammalian cells

Depositor

Zhou Songyang

Article

Chen et al Nat Commun. 2020 Jun 19;11(1):3136. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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vector-dendra2 PCDNA

Plasmid

#121134

Purpose

mammalian cell expression

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

empty cassete for contol of miR expression

Expression

Bacterial

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pGZ12.0501

Plasmid

#141164

Purpose

TcTT2 in Ti plasmid binary vector pGZ12.0501 (KF871320.1)

Depositor

Mark Guiltinan

Article

Florez et al BMC Plant Biol. 2015 May 16;15:121. doi:

Insert

TcTTC

Expression

Plant

Promoter

E12-omega

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pML2003

Plasmid

#193255

Purpose

Propionate sensor expressing eYFP reporter

Depositor

Lauren Andrews

Article

Lebovich et al Front Bioeng Biotechnol. 2022 Aug 25;10:

Inserts

prpR
eYFP

Use

Synthetic Biology

Promoter

Pprp and Synthetic promoter P6

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-NODAL-matMYC

Plasmid

#115639

Purpose

For targeted integration and inducible expression of human NODAL (with an N-terminal MYC tag on the mature peptide) using doxycycline

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Mammalian

Promoter

TRE3G

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-7SK-Blast

Plasmid

#183620

Purpose

lentiCRISPRv2 derivative with modified 7SK promoter driving sgRNA and encoding Blasticidin resistance. Compatible with inserts designed for lentiCRISPRv2.

Depositor

Bill Sugden

Article

Hutcheson et al ACS Synth Biol. 2022 Jun 29. doi: 10.102

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Modified 7SK

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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RCAS (A) cTbx3 del2 (CT#645)

Plasmid

#13921

Depositor

Cliff Tabin

Article

Logan et al Development. 1998 Aug . 125(15):2825-35.

Insert

Tbx3 del (TBX3 Chicken)

Use

Retroviral; Avian expression

Mutation

Truncated Tbx3. Putative dominant negative.

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only