We narrowed to 9,829 results for: pho;
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Plasmid#116864PurposeRecruitable PI(3,4)P2 4-phosphataseDepositorAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only
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Sema6a.a-Fc-His
Plasmid#72163PurposeExpresses the extracellular region of the Sema6A, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
psicheck2-PHLPP2mut
Plasmid#203606PurposeReporter plasmid carrying PHLPP2 3'UTR with mutated miR-27b target sequencesDepositorInsertPH domain and leucine rich repeat protein phosphatase 2 (PHLPP2 Human)
UseLuciferaseExpressionMammalianMutationmiR-27b target sequences in the 3'UTR were m…PromoterSV40Available SinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1-0-GFP
Plasmid#41662DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
TagsGFPExpressionMammalianMutationC-terminal hydrophobic isoleucine residue added a…PromoterCMVAvailable SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
His-DNPH1
Plasmid#203224PurposeFor protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)
Plasmid#195741PurposeBase editing for making a pre-stop codon in mmoX using phenol inducible systemDepositorArticleInsertDi-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPo (phoenol-inducible promoter)Available SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
SiriusGFP
Plasmid#123201PurposeWe have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.DepositorInsertSiriusGFP
UseMouse TargetingTagsN/AExpressionBacterial and MammalianMutationeGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…PromoterCMVAvailable SinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
p6608 MSCV-IP N-HAonly CHAF1B
Plasmid#34901DepositorAvailable SinceFeb. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
LiPOP1
Plasmid#168264PurposeA single-component optogenetic tool for photoswitchable necroptosisDepositorInsertMLKL(1-125) (MLKL Human)
TagsmCherry, CRY2-PHRExpressionMammalianMutationH15A, K16A, R17APromoterCMVAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGLACTE-PseudoHT51
Plasmid#99257PurposeContains expanded CGG repeats (98 repeats with 2 AGG interruptions) within the 5'UTR of FMR1. Internal control with synthetic primer sequence for capillary electrophoresisDepositorInsertFMR1 (FMR1 Human, Synthetic)
MutationContains synthetic, non-human, primer sequence do…Available SinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
Plxna1-AP-His
Plasmid#71996PurposeExpresses the extracellular region of the PlexinA1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry1-Mito-7
Plasmid#57189PurposeLocalization: Mitochondria, Excitation: 400 / 504, Emission: 515 / 517DepositorAvailable SinceJan. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDendra2-5'UTR(GAPDH)-Dendra2-3'UTR(GAPDH)
Plasmid#182368PurposeDendra2-based translation reporterDepositorInsertglyceraldehyde-3-phosphate dehydrogenase (Gapdh Mouse)
TagsPal-Dendra2ExpressionMammalianPromoterCMV promoterAvailable SinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
L2096-GPA-BRET-Biosensor-Retrovirus-TD146
Plasmid#222878PurposeRetroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with BRET (split NanoLuciferase reconstitution [11S/114 fragment]). WT Glycophorin A (GPA) scaffold.DepositorInsertFRB-GPA-NanoLuc114-CyOFP1-T2A-FKBP-GPA-NanoLuc11S
UseRetroviralTagsMycExpressionMammalianPromoterMSCV LTRAvailable SinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Sema7a-Fc-His
Plasmid#72175PurposeExpresses the extracellular region of the Sema7A protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
p2R3a-3AT
Plasmid#71273PurposeEntry clone containing 3AT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertT3A ribulose-1,5-bisphosphate carboxylase 3A subunit terminator
UseGateway CloningAvailable SinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
FAK2_HUMAN_D0
Plasmid#79747PurposeThis plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
S1PR1-DuET
Plasmid#213374PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
mEosEM-E_Lamin A
Plasmid#226338PurposePhotoswitchable fluorescent protein for correlative light-electron microscopy; nuclear envelope-targeted mEosEM-E for mammalian expressionDepositorAvailable SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-PTPRD-G446E
Plasmid#25643DepositorInsertProtein tyrosine phosphatase receptor-type delta (PTPRD Human)
UseLentiviralExpressionMammalianMutationtumor-derived mutation created by site-directed m…Available SinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only