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pComb8

Plasmid

#63889

Purpose

For phage display of antibody libraries by fusion to modified geneVIII.

Depositor

Carlos Barbas

Publication

Barbas CF et al Proc Natl Acad Sci U S A. 1991 Sep 15;88

Type

Empty backbone

Use

Phage display

Tags

geneVIII

Expression

Bacterial

Promoter

lacZ

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3.1 6A NLRP1 UPA-CARD-FLAG WT

Plasmid

#163979

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD with a C-terminal FLAG tag

Depositor

Hao Wu

Publication

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Available since

Jan. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV_BiSSTe10_ChR2_mCherry

Plasmid

#213815

Purpose

AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting enhancer. Alias: pAAV_S9E10_ChR2_mCherry

Depositor

Gordon Fishell

Publication

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has service

AAV1 and AAV PHP.eB

Insert

Channelrhodopsin 2-mCherry

Use

AAV

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV_BiSSTe4_ChR2_mCherry

Plasmid

#213945

Purpose

AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting enhancer. Alias: pAAV_WDS0004_ChR2_mCherry

Depositor

Gordon Fishell

Publication

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has service

AAV1 and AAV PHP.eB

Insert

Channelrhodopsin 2-mCherry

Use

AAV

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam E2F6

Plasmid

#37965

Depositor

Jacqueline Lees

Publication

Trimarchi et al Proc Natl Acad Sci U S A. 2001 Feb 13;98

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

E2F6 (E2F6 Human)

Expression

Mammalian

Available since

July 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAV-P(Per2)-DIO-intron2-Venus-NLS-D2

Plasmid

#110057

Purpose

Per2 transcription fluorescence reporter

Depositor

Eric Erquan Zhang

Publication

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Per2 promoter and Venus (Per2 Mouse)

Use

AAV and Cre/Lox

Tags

NLS-D2

Expression

Mammalian

Promoter

Per2

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pET32a-HD16Q

Plasmid

#11487

Depositor

Pamela Bjorkman

Publication

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

huntingtin, exon 1, 16 glutamines (HTT Human)

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

huntingtin exon 1 with 16 glutamines, vector m…

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA3.1 6A NLRP1 CARD-FLAG WT

Plasmid

#163978

Purpose

Mammalian expression vector encoding human NLRP1 CARD with a C-terminal FLAG tag

Depositor

Hao Wu

Publication

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Available since

Jan. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AU1-mTOR-L1460P

Plasmid

#19995

Depositor

Fuyuhiko Tamanoi

Publication

Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mammalian target of rapamycin-L1460P (Mtor Rat)

Tags

AU1

Expression

Mammalian

Mutation

Changed Leucine 1460 to Proline

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP

Plasmid

#137148

Purpose

Intersectional viral expression of Arch3.3-EYFP in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Publication

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has service

AAV8

Insert

Con/Fon-Arch3.3-p2a-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-GCaMP6F

Plasmid

#137123

Purpose

Intersectional viral expression of GCaMP6F in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Publication

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has service

AAV8

Insert

Con/Foff 2.0-GCaMP6F

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

Removed RSET tag

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pFasBac+GFP-CAMSAP1

Plasmid

#59036

Purpose

for baculovirus expression of CAMSAP1 in Sf9 cells

Depositor

Ron Vale

Publication

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP1 (CAMSAP1 Human)

Tags

His and eGFP

Expression

Insect

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pLV-Y701F-STAT1

Plasmid

#71453

Purpose

Lentiviral expression of Y701F-STAT1

Depositor

George Stark

Publication

Cheon et al Proc Natl Acad Sci U S A. 2009 Jun 9;106

Insert

STAT1 (STAT1 Human)

Use

Lentiviral

Tags

FLAG

Mutation

Y701F

Promoter

CMV

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pLCAG EGFP

Plasmid

#14857

Depositor

Inder Verma

Publication

Pfeifer et al Proc Natl Acad Sci U S A. 2002 Feb 19. 9

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Available since

May 11, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pSAR-MT-APC

Plasmid

#16487

Depositor

Bert Vogelstein

Publication

Morin et al Proc Natl Acad Sci U S A. 1996 Jul 23. 9

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

APC (APC Human)

Expression

Mammalian

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pACU2

Plasmid

#31223

Depositor

Yuh-Nung Jan

Publication

Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

Type

Empty backbone

Expression

Insect

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pGCS-N3(3xFLAG)-CRYAB

Plasmid

#258355

Purpose

Flag-tagged fusion protein used for the cell-based assay autoantibody validation assays

Depositor

Christopher Bartley

Insert

CRYAB (alpha-B crystallin) (CRYAB Human)

Tags

3XFlag

Expression

Mammalian

Available since

Aug. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pGCS-CRYAB-C3m(3xFLAG)

Plasmid

#258356

Purpose

Flag-tagged fusion protein used for the cell-based assay autoantibody validation assays

Depositor

Christopher Bartley

Insert

CRYAB (alpha-B crystallin) (CRYAB Human)

Tags

3XFlag

Expression

Mammalian

Available since

Aug. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc-NL-GW-CRYAB

Plasmid

#258339

Purpose

Myc-nanoluciferase fusion protein used in luciferase immunoprecipitation system (LIPS) autoantibody validation assays

Depositor

Christopher Bartley

Insert

CRYAB (alpha-B crystallin) (CRYAB Human)

Tags

cmyc-NanoLuc

Expression

Mammalian

Available since

Aug. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 CRYAB-GW-NL-myc

Plasmid

#258340

Purpose

Myc-nanoluciferase fusion protein used in luciferase immunoprecipitation system (LIPS) autoantibody validation assays

Depositor

Christopher Bartley

Insert

CRYAB (alpha-B crystallin) (CRYAB Human)

Tags

myc-nanoluciferase

Expression

Mammalian

Available since

Aug. 14, 2026

Availability

Academic Institutions and Nonprofits only

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