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C1-FT-mCitrine-EEVEE-CaaX

Plasmid

#155225

Purpose

MPAct with increased linker flexiblity

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with a codon optimized EEVEE and CaaX seq derived from Kras4b

Tags

mCitrine

Expression

Mammalian

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pPN441

Plasmid

#137873

Purpose

PiggyBac vector for expression of 3xgRNA targeting TCF4 for CRISPRi; mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMTS_mScarlet-H_N1

Plasmid

#85058

Purpose

In vivo visualization of the mitochondria (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

MTS (COX8A Human)

Tags

mScarlet-H

Expression

Mammalian

Mutation

aa1-29

Promoter

CMV

Available Since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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Plxna3-Fc-His

Plasmid

#72124

Purpose

Expresses the extracellular region of the PlexinA3 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxna3 (Plxna3 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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Sema4d-Fc-His

Plasmid

#72157

Purpose

Expresses the extracellular region of the Sema4D protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4d (Sema4d Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pEYFPC1-ECFP/GgVcl 1-419 control 1 FRET

Plasmid

#46277

Depositor

Susan Craig

Article

Chen et al J Cell Biol. 2005 May 9;169(3):459-70.

Insert

ECFP + Vcl 1-419 (VCL Chicken)

Tags

EYFP

Expression

Mammalian

Mutation

aa 1-419

Promoter

CMV

Available Since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only

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-962 human cyclin D1 promoter EtsB site mutant pGL3Basic

Plasmid

#32730

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 (CCND1 Human)

Use

Luciferase

Mutation

-778 EtsB binding site AGGAA deletion

Promoter

-962 CCND1

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
pDONR223-LOC652799

Plasmid

#23394

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LOC652799 (LOC652799 Human)

Use

Gateway Cloning

Mutation

S34Y L35M P114A

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pIS1 DICER1 short UTR

Plasmid

#21648

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Dicer 1 (DICER1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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2xFRB-myc

Plasmid

#20227

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

2xFRB (MTOR Human)

Tags

myc

Expression

Mammalian

Mutation

1 FRB copy has a T2098L point mutation

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pLPC-NFLAG TRF1 deltaA

Plasmid

#16063

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Mutation

Deleted acidic domain (aa1-66)

Available Since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pGL2 p15 4xSBR2 MutSBE

Plasmid

#15707

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

p15 4xSBR2 (CDKN2B Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutant SBE (Smad binding element)

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pUC19 PKC epsilon

Plasmid

#8442

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Mar 25;268(9):6090-6.

Insert

Mouse Protein Kinase C epsilon (Prkce Mouse)

Expression

Bacterial

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
HP17deltaP8

Plasmid

#235447

Purpose

Helper plasmid for regulated phage transfer

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

Coding part of the M13KO7 helper phage genome, except gene VIII

Expression

Bacterial

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-Atoh1

Plasmid

#158529

Purpose

Expresses mouse Atoh1 in mammalian cells

Depositor

Neil Segil

Article

Menendez et al Elife. 2020 Jun 30;9. pii: 55249. doi: 1

Insert

Atonal BHLH Transcription Factor 1 (Atoh1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-MCART2

Plasmid

#133250

Purpose

Express FLAG-tagged MCART2 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

MCART2 (SLC25A52 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

codon-optimized for expression in human cells

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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EPHB4-2A-EGFP

Plasmid

#139968

Purpose

Donor vector to knockin EGFP (separated by 2A) into EPHB4 allele

Depositor

James Thomson

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

Insert

Human EPHB4 knockin homology arms (EPHB4 Human)

Expression

Mammalian

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pBEAST-J23101-sfGFP

Plasmid

#116917

Purpose

Strong constitutive expression of super-folder GFP for cell-free expression

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Promoter

J23101

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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BII-ChPtW-EGFP

Plasmid

#133400

Purpose

Piggybac vector for constitutive expression of EGFP

Depositor

Blair Madison

Article

Strubberg et al Stem Cell Reports. 2018 Aug 14;11(2):410

Insert

EGFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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V1-MESA-45F-M-dCas9

Plasmid

#84504

Purpose

MESA target chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, M cleavage sequence, and dCas9-VP64

Depositor

Joshua Leonard

Article

Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

Insert

V2-MESA-35F-M-dCas9

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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