We narrowed to 13,211 results for: CAR

1. pOTTC374 - pAAV EF1a DIO iRFP

   Plasmid

   #47626

   Purpose

   An AAV vector that expresses iRFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   Infrared fluorescent protein
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT3TS-zSpG

   Plasmid

   #179316

   Purpose

   In vitro transcription of SpG-Cas9 mRNA zebrafish codon-optimized from T3 promoter

   Depositor

   Miguel Angel Moreno-Mateos

   Article

   Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

   Insert

   Zebrafish codon-optimized Cas9 variant SpG
   

   Use

   CRISPR; In vitro transcription by t3 promoter

   Tags

   SV40-NLS

   Mutation

   SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. V5-ERG-R367K

   Plasmid

   #118624

   Purpose

   Expresses V5 tagged ERG R367K (DNA binding mutant) protein

   Depositor

   William Hahn

   Article

   Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

   Insert

   ERG (R367K DNA binding mutant) (ERG Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   R367K

   Promoter

   EF1a

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-IDH2-R172K

   Plasmid

   #87927

   Purpose

   Expresses IDH2 (R172K mutant) in mammalian cells

   Depositor

   Reproducibility Project Cancer Biology

   Article

   Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

   Insert

   isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)
   

   Tags

   none

   Expression

   Mammalian

   Mutation

   R172K

   Promoter

   CMV

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pscALPSpuro-MjACE2 (Pangolin)

   Plasmid

   #158084

   Purpose

   Expresses pangolin ACE2 in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   Pangolin ACE2 (ACE2 )
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV pCAG-FLEX2-tTA2-WPRE-bGHpA

   Plasmid

   #65458

   Purpose

   Can be used to generate AAV virus that will express the tetracycline transactivator tTA2 from the CAG promoter in a Cre-dependent manner

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   tTA2
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTwist-SARS-CoV-2 Δ18 D614G

   Plasmid

   #164437

   Purpose

   Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids and D614G mutation for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   SARS-CoV-2 Spike D614G variant truncated to remove 18 amino acids (S )
   

   Expression

   Mammalian

   Available Since

   Feb. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT3TS-zSpRY

   Plasmid

   #179317

   Purpose

   In vitro transcription of SpRY-Cas9 mRNA zebrafish codon-optimized from T3 promoter

   Depositor

   Miguel Angel Moreno-Mateos

   Article

   Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

   Insert

   Zebrafish codon-optimized Cas9 variant SpRY
   

   Use

   CRISPR; In vitro transcription by t3 promoter

   Tags

   SV40-NLS

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-EFS-NTID194-GSQ-PMLivaWT-P2A-BLAST

   Plasmid

   #208038

   Purpose

   Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; WT); to perform SUMO-ID; selection with blasticidin

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

   Insert

   PML (isoform IVa) (PML Human)
   

   Use

   Lentiviral

   Tags

   FLAG, N-terminal Split-TurboID

   Promoter

   EFS

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hsyn-Jaws-KGC-GFP-ER2

    Plasmid

    #65014

    Purpose

    AAV-mediated expression of Jaws under the human synapsin promoter

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Has Service

    AAV Retrograde, AAV5, and AAV8

    Insert

    Jaws-KGC-GFP-ER2
    

    Use

    AAV

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    human synapsin

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAGT9054

    Plasmid

    #219430

    Purpose

    Transcriptional unit (MoClo-position 2): 2x35Ss_Omega enhancer_PapE-4LF2-N-SpCas9i-N_tOCS

    Depositor

    Tom Schreiber

    Article

    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

    Insert

    LB_2x35Ss-omega enhancer:PapE-4xLF2-NLS-SpCas9i-NLS:tOCS_RB (Position 2)
    

    Use

    CRISPR; Moclo compatible level 1 module (position…

    Tags

    PapE-4LF2

    Expression

    Plant

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRM053 His-TEV-Cas9 triNLS 0C

    Plasmid

    #196244

    Purpose

    Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

    Depositor

    Ross Wilson

    Article

    Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA, His6 (N terminal on backbone), NLS, Nucleopla…

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28b-SpG-His

    Plasmid

    #179318

    Purpose

    Plasmid for bacterial expression and purification of SpG-Cas9

    Depositor

    Julián Cerón, Miguel Angel Moreno-Mateos

    Article

    Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

    Insert

    Human codon-optimized Cas9 variant SpG
    

    Use

    CRISPR

    Tags

    6xHis and SV40-NLS

    Expression

    Bacterial

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSecTaq2-hProS-Myc-6xHis

    Plasmid

    #226524

    Purpose

    Expresses TAM Receptor Ligand human PROS1

    Depositor

    Raymond Birge, Sergei Kotenko

    Article

    Geng et al Front Immunol. 2017 Nov 10;8:1521. doi:

    Insert

    Human PROS1 (PROS1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-Syn-Jaws-KGC-tdTomato-ER2

    Plasmid

    #153535

    Purpose

    AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the Syn promoter.

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-KGC-tdTomato-ER2
    

    Use

    AAV

    Tags

    ER2, KGC, and tdTomato

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    Syn

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR-BLINK2

    Plasmid

    #117075

    Purpose

    BLINK2 gene in pDONR vector for Gateway cloning system

    Depositor

    Anna Moroni

    Article

    Alberio et al Nat Methods. 2018 Nov;15(11):969-976. do

    Insert

    BLINK2
    

    Use

    Gateway cloning system

    Mutation

    glutamine 112 changed into aspartate (original LO…

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Mouse Ism1 with C-terminal Myc-6X-his tag

    Plasmid

    #173046

    Purpose

    Expresses Ism1 in mammalian cells

    Depositor

    Katrin Svensson

    Article

    Jiang et al Cell Metab. 2021 Jul 28. pii: S1550-4131

    Insert

    Ism1 (Ism1 Mouse)
    

    Expression

    Mammalian

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSET-iSeroSnFR

    Plasmid

    #128483

    Purpose

    Fluorescent reporter for serotonin (bacterial expression)

    Depositor

    Lin Tian

    Article

    Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

    Insert

    iSeroSnFR
    

    Tags

    His, T7, and cpsfGFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOTTC469 - pAAV EF1a DIO eNpHR3.0-iRFP

    Plasmid

    #47631

    Purpose

    An AAV vector that expresses engineered halorhodopsin 3.0 (fused to iRFP) in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    eNpHR3.0
    

    Use

    AAV and Cre/Lox

    Tags

    iRFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCSIIpuro-AREG-ScNeo

    Plasmid

    #209897

    Purpose

    To monitor the status of Amphiregulin, the plasmid encodes a recombinant AREG fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    AREG-ScNeo (AREG Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen and mScarlet

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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