We narrowed to 12,359 results for: NSI

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pb-NES-ZapCV5 (cpV143)

Plasmid

#112061

Purpose

Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Low affinity to free zinc (Kd ~ 300nM, n = 0.55) due to mutation of the zinc binding Cys residues to His.

Depositor

Amy Palmer

Article

Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi

Insert

NES-ZapCV5

Tags

Nuclear Export Signal (NES) derived from HIV-1

Expression

Mammalian

Mutation

ECFP gene is truncated 36 bp at 3' end. Venu…

Promoter

CBA

Available Since

July 9, 2018

Availability

Academic Institutions and Nonprofits only
DOT1L-CC3

Plasmid

#111754

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Article

Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

Insert

DOT1L (DOT1L Human)

Tags

8xHis-Thrombin-SUMO-TEV

Expression

Bacterial

Mutation

Contains coiled coil #3 (residues 613-661)

Promoter

T7

Available Since

June 14, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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DOT1L-CC1

Plasmid

#111756

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Article

Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

Insert

DOT1L (DOT1L Human)

Tags

8xHis-Thrombin-SUMO-TEV

Expression

Bacterial

Mutation

contains coiled coil #1 (residues 506-555)

Promoter

T7

Available Since

June 14, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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DOT1L-CC2

Plasmid

#111755

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Article

Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

Insert

DOT1L (DOT1L Human)

Tags

8xHis-Thrombin-SUMO-TEV

Expression

Bacterial

Mutation

Contains coiled coil #2 (residues 560-608)

Promoter

T7

Available Since

June 14, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pAav-IQGAP1-PQS1-3xFLAG

Plasmid

#84885

Purpose

rAAV-based template for genome engineering of the IQGAP1 C-terminus containing PQS1 and 3xFLAG tags and a selection cassette

Depositor

Sven Eyckerman

Article

Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

Inserts

IQGAP1 genomic region HR1 (IQGAP1 Human)
IQGAP1 genomic region HR2

Use

AAV

Tags

PQS1 3xFLAG

Promoter

no

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pMCh-1540

Plasmid

#82433

Purpose

Plasmid for expression of GST-tagged human-Nematostella GW182 11W11A chimera in mammalian cells

Depositor

Marina Chekulaeva

Article

Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

Insert

hs-nvGW182 11W11A chimera

Tags

GST

Expression

Mammalian

Mutation

aa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …

Promoter

EF1A

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-puro/RAF1-P207S

Plasmid

#131726

Purpose

Mammalian Expression of RAF1

Depositor

Jacques De Grève

Article

Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

Insert

RAF1 (RAF1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

P207S

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE

Plasmid

#125713

Purpose

Cre-dependent vector expressing an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

Depositor

Ofer Yizhar

Article

Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

Has Service

AAV1 and AAV5

Insert

eOPN3

Use

AAV and Cre/Lox

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Mutation

deleted amino acids 331-429

Promoter

hSyn1

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV uN2C GFP (Ctl)

Plasmid

#224355

Purpose

Express GFP tagged upsteram ORF of NOTCH2NLC with a control size (12x) of GGC repeats

Depositor

Nicolas Charlet-Berguerand

Article

Boivin et al Neuron. 2021 Jun 2;109(11):1825-1835.e5.

Insert

uN2C

Use

AAV

Tags

eGFP

Expression

Mammalian

Mutation

Codon-optimized for expression in human, so no pu…

Promoter

CMV + chimeric intyron (CAG)

Available Since

Oct. 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Tet-pLKO-puro-Scrambled

Plasmid

#47541

Purpose

Tet inducible scrambled shRNA.

Depositor

Charles Rudin

Article

Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

Insert

Scrambled shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pDisplay-iSeroSnFR

Plasmid

#128484

Purpose

Fluorescent reporter for serotonin (mammalian expression, membrane localization tag)

Depositor

Lin Tian

Article

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Insert

iSeroSnFR

Tags

Ig-kappa leader, Myc, PDGFR + enhanced ER export,…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSYN1-NES-OCaMP-WPRE

Plasmid

#224934

Purpose

pAAV (non-flex) for expressing OCaMP, an orange calcium indicator, in mammalian cells and in vivo. Contains hSyn promoter and RSET leader sequence peptide

Depositor

Alexander Lohman

Article

Aggarwal et al bioRxiv 2025.07.28.667269; doi: https://

Insert

OCaMP

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSynI

Available Since

July 28, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-hSyn-FLEX(cre)-OCaMP-WPRE

Plasmid

#229853

Purpose

pAAV vector for Cre-dependent OCaMP (orange calcium indicator) expression under the control of hSyn promoter

Depositor

Alexander Lohman

Article

Aggarwal et al bioRxiv 2025.07.28.667269; doi: https://

Insert

OCaMP

Use

AAV

Tags

6xHis

Expression

Mammalian

Available Since

Aug. 25, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pGP-pcDNA3.1 Puro-CAG-Voltron2

Plasmid

#172909

Purpose

Mammalian expression of voltage sensor

Depositor

GENIE Project

Article

Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

Insert

Voltron2

Expression

Mammalian

Mutation

A122D

Promoter

CAG

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-CMV-PE2-IRES-ZeoR

Plasmid

#207352

Purpose

Lentiviral transfer plasmid containing CMV-driven expression cassette encoding PE2 enzyme for prime editing.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

Prime editor 2 (PE2) enzyme

Use

Lentiviral

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE

Plasmid

#125712

Purpose

Expresses an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

Depositor

Ofer Yizhar

Article

Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

Has Service

AAV Retrograde, AAV1, and AAV5

Insert

eOPN3

Use

AAV

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Mutation

deleted amino acids 331-429

Promoter

short version of αCamKinase II promoter (0.4kb)

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1_c-SRC

Plasmid

#171850

Purpose

pEGFP-N1_c-SRC

Depositor

Michele Pagano

Article

Moro et al Nat Cell Biol. 2020 Sep;22(9):1130-1142.

Insert

c-SRC (SRC Human)

Tags

GFP

Expression

Mammalian

Mutation

Added a linker sequence in 3' of c-SRC: GSGS

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-pps-UvsX

Plasmid

#236046

Purpose

pET19b-pps-UvsX

Depositor

Luis Gabriel Brieba

Article

Cordoba-Andrade et al PeerJ. 2025 Aug 13;13:e19758. doi: 10.77

Insert

UvsX

Tags

His tag

Expression

Bacterial

Promoter

T7 RNA polymerase

Available Since

Aug. 1, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET19b-pps-gp32

Plasmid

#236044

Purpose

gp32, synthetic gene

Depositor

Luis Gabriel Brieba

Article

Cordoba-Andrade et al PeerJ. 2025 Aug 13;13:e19758. doi: 10.77

Insert

gp32

Tags

His tag

Expression

Bacterial

Available Since

July 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET19b-pps-UvsY

Plasmid

#236045

Purpose

pET19b-pps-UvsY

Depositor

Luis Gabriel Brieba

Article

Cordoba-Andrade et al PeerJ. 2025 Aug 13;13:e19758. doi: 10.77

Insert

UvsY

Tags

His tag

Expression

Bacterial

Promoter

T7 RNA polymerase

Available Since

July 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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