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pCW-codon optimized catalytically inactive PHGDH

Plasmid

#154903

Purpose

Expresses codon optimized catalytically inactive PHGDH in mammalian cells

Depositor

Michael Pacold

Publication

Ngo et al Cancer Discov. 2020 Sep;10(9):1352-1373.

Insert

Phosphoglycerate dehydrogenase (PHGDH Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Three mutations: D175N, R236K, H283A

Promoter

TRE

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AiP11851-pAAV-hDLX-minBG-iCre-4X2C-WPRE3-BGHpA (Alias: CN1851)

Plasmid

#164450

Purpose

AAV vector for Cre expression in forebrain GABAergic interneurons. For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors.

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Has service

AAV PHP.eB

Insert

iCre

Use

AAV

Promoter

minBetaGlobin

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-Ef1a-Con/Foff 2.0-mCherry

Plasmid

#137133

Purpose

Intersectional viral expression of mCherry in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Publication

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has service

AAV8

Insert

Con/Foff 2.0-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.NES-jRGECO1b.WPRE.SV40

Plasmid

#100855

Purpose

AAV expression of Cre recombinase-activated jRGECO1b, a red fluorescent calcium sensor protein, from the CAG promoter

Depositor

Douglas Kim, GENIE Project

Publication

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

jRGECO1b

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-P(Cry1)-forward-intron336-Venus-NLS-D2

Plasmid

#110054

Purpose

Cry1 transcription fluorescence reporter

Depositor

Eric Erquan Zhang

Publication

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Cry1 promoter and Venus (Cry1 Mouse)

Use

AAV

Tags

NLS-D2

Expression

Mammalian

Promoter

Cry1

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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TRAF6mut-UTR

Plasmid

#15094

Depositor

David Baltimore

Publication

Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TRAF6 3'UTR (TRAF6 Human)

Use

Luciferase

Expression

Mammalian

Mutation

4-bp substitutions in the miR-146 target sites

Available since

June 6, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pHAGE-ERBB3-V104M

Plasmid

#116363

Purpose

Lentiviral expression of ERBB3 V104M

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB3 (ERBB3 Human)

Use

Lentiviral

Mutation

V104M

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pQE-80L MBP-SspB Micro

Plasmid

#60410

Purpose

E. coli expression of SspB Micro

Depositor

Brian Kuhlman

Publication

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

SspB micro

Tags

His-MBP-TEV

Expression

Bacterial

Mutation

R73Q, Y11K and A15E

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pQE-80L iLID (C530M)

Plasmid

#60408

Purpose

E. coli expression of iLID (C530M)

Depositor

Brian Kuhlman

Publication

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

iLID C530M

Tags

His

Expression

Bacterial

Mutation

C530M

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles
GFP-WT (AF41)

Plasmid

#21367

Depositor

Gregory Germino

Publication

Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

wild-type sequence*

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pH2B-LSSmKate2-N1

Plasmid

#31903

Depositor

Vladislav Verkhusha

Publication

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

H2B-LSSmKate2

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

Promoter

CMV

Available since

Sept. 20, 2011

Availability

Academic Institutions and Nonprofits only

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Flag‐hTim1

Plasmid

#49207

Purpose

expresses Flag-tagged human Tim1

Depositor

Lawrence Kane

Publication

de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

Insert

Tim1 (HAVCR1 Human)

Tags

FLAG and signal sequence

Expression

Mammalian

Mutation

Tim1 lacks its own signal sequence

Promoter

EF1a

Available since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pGSKU

Plasmid

#72243

Purpose

Contains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoter

Depositor

Michael Resnick, Francesca Storici

Publication

Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

Inserts

KlURA3
kanMX4
I-SceI

Expression

Bacterial

Available since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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MELOPS

Plasmid

#80151

Purpose

Genetically encoded melanosome-localized pH sensor.

Depositor

Santiago Di Pietro

Publication

Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

OCA2 (OCA2 Human)

Tags

mNectarine in first luminal loop

Expression

Mammalian

Mutation

nonpathologic polymorphism D257A, pore mutation V…

Promoter

CMV

Available since

Sept. 20, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles
Flag_HsIRE1a_pBabePuro

Plasmid

#54337

Purpose

N-terminally FLAG tagged human IRE1a in retroviral pBabe puro vector

Depositor

David Ron

Publication

Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

Insert

IRE1a (ERN1 Human)

Use

Retroviral

Tags

FLAG and preprotrypsin signal sequence

Expression

Mammalian

Mutation

The endogenous IRE1a signal sequence (aa1-18) was…

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLEGFP-S727A-STAT3

Plasmid

#71446

Purpose

Retroviral expression of S727A-STAT3. Please note that this plasmid contains S727A-STAT3 tagged with FLAG, not GFP.

Depositor

George Stark

Publication

Dasgupta et al Proc Natl Acad Sci U S A. 2015 Mar 31;11

Insert

STAT3 (STAT3 Human)

Use

Retroviral

Tags

FLAG

Mutation

S727A

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pORTMAGE-Pa1

Plasmid

#138475

Purpose

Enables high-efficiency recombineering in P. aeruginosa

Depositor

George Church

Publication

Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

Inserts

PapRecT
mutL E36K

Expression

Bacterial

Mutation

E36K

Available since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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WZLneo-C/EBPα

Plasmid

#34567

Depositor

Kai Ge

Publication

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

C/EBPα (Cebpa Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available since

March 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBAD/HisD-LSSmKate2

Plasmid

#31909

Depositor

Vladislav Verkhusha

Publication

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

LSSmKate2

Tags

HIS

Expression

Bacterial

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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IRAK1mut-UTR

Plasmid

#15096

Depositor

David Baltimore

Publication

Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

IRAK1 3'UTR (IRAK1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

4-bp substitutions in the miR-146 target sites

Available since

June 6, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles