We narrowed to 25,334 results for: Spr

Showing: 9401 - 9420 of 25334 results

Results per page:

Sort results by:

LentiGuidPuro-hTP73_sgRNA-1

Plasmid

#88855

Purpose

CRISPR KO of Trp73

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp73

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6T7G

Plasmid

#71463

Purpose

Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (1 G at initiation)

Depositor

Willie Mark

Article

Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi

Type

Empty backbone

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

human U6/phage T7

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
p1195-pssAAV.U1a.hNme2Cas9

Plasmid

#129534

Purpose

AAV vector expressing Nme2Cas9

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Insert

human codon-optimized Nme2Cas9

Use

AAV

Tags

2xNLS and NLS-3xHA-NLS

Promoter

U1a

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pN-PITCh-FAID

Plasmid

#127885

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter FAID tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-H3F-AID (PPP2CA Human)

Use

CRISPR

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPPC005

Plasmid

#171140

Purpose

For integration of XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

XylS-Pm

Use

CRISPR

Expression

Bacterial

Promoter

Pm

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPbuCas13b

Plasmid

#184565

Purpose

Inducible expression of PbuCas13b nuclease in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

Cas13b nuclease from Prevotella buccae

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas2C

Plasmid

#82374

Purpose

dCas9 expressed from aTc-inducible promoter in acsA, C RBS: TGGACA

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Promoter

PEZ3

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_ctrA

Plasmid

#133339

Purpose

for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_ctrA

Use

CRISPR

Promoter

constitutive

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiGuidPuro-hTP63_sgRNA-1

Plasmid

#88854

Purpose

CRISPR KO of Trp63

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp63

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPSA5Cas9syn2

Plasmid

#206963

Purpose

S. aureus genome editing vector removed these two motifs AGGNNNNNGAT, CCAYNNNNNNTGT, CCAYNNNNNNGTA, GGANNNNNNNNTCG, and GWAGNNNNNNGAT

Depositor

Daijie Chen

Article

Yang et al ACS Synth Biol. 2023 Oct 13. doi: 10.102

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_212

Plasmid

#133457

Purpose

U6 promoter expresses customizable Spyo-guide; EFS promoter expresses SaurCas9 and 2A site provides puromycin resistance

Depositor

John Doench

Article

DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

Insert

control guide

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
NAX_CAGGS_CAS9_VPR_Puro

Plasmid

#167895

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOC1 Halo-Actb KI

Plasmid

#183426

Purpose

CreON knock-in for Halo-beta-actin (amino acid position: before start codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and Halo donor

Use

CRISPR

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas34

Plasmid

#82386

Purpose

sgRNA targeting YFP expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0271_pET30_pCat-tetR-Term-ptetA-sfGFP-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

Plasmid

#116955

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac and sfGFP under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAcCAST-sgRNA_entry (CJT83)

Plasmid

#181785

Purpose

Expresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

AcTnsB, AcTnsC, AcTniQ, AcCas12k

Expression

Bacterial

Promoter

Lac and J23119

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-PpU6P-sgRNA-L1L2

Plasmid

#113735

Purpose

Gateway entry vector containing attL1 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR; Gateway entry vector

Promoter

P. patens U6 promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
gh19

Plasmid

#106703

Purpose

expression of gRNA targeting GALNT19

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GALNT19

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB700 lenti gRNA Blasto_zhang2.0

Plasmid

#167912

Purpose

Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ-NF-L3-NF-L1-dMb2Cas12a

Plasmid

#177686

Purpose

Gateway entry clone (attL1 & attR5) for a single chain FokI-dMb2Cas12a genome editing

Depositor

Yiping Qi

Article

Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

Insert

2xFokI-dMb2Cas12a

Use

CRISPR; Gateway compatible 2xfoki-dmb2cas12a entr…

Tags

NLS

Expression

Plant

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge