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pMDC32B-OsMIR390-B/c

Plasmid

#61467

Purpose

Plant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.

Depositor

James Carrington

Article

Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1

Insert

OsMIR390-B/c

Expression

Plant

Mutation

O. sativa MIR390 precursor sequence including a c…

Promoter

2x35S

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf37

Plasmid

#12725

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf37

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf48

Plasmid

#12736

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf48

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA 3.1(-) mouse Egr4

Plasmid

#108096

Purpose

Expresses mouse Egr4 in mammalian cells

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr4 (Egr4 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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SCARF-bio-His

Plasmid

#51826

Purpose

Expresses full-length Endothelial cells scavenger receptor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

SCARF (SCARF1 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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RhopH3-bio

Plasmid

#47781

Purpose

Expresses enzymatically monobiotinylated full-length RhopH3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Insert

Codon-optimised RhopH3

Tags

enzymatic biotinylation sequence and rat Cd4 d3+4

Expression

Mammalian

Mutation

Exogenous signal peptide of mouse origin, changed…

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pEJS1026-pMCSG7-HpaCas9

Plasmid

#121540

Purpose

Expresses a 6X-His tagged type II-C Cas9 from H. parainfluenzae in bacterial cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

HpaCas9

Expression

Bacterial

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pEJS482-pCDest2-AcrIIC3Nme-mTagBFP2-IRES

Plasmid

#85750

Purpose

Expresses Type II-C anti-CRISPR protein, AcrIIC3Nme from Neisseria meningitidis, and mTagBFP2

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Inserts

Type II-C anti-CRISPR AcrIIC3Nme
mTagBFP2

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf42

Plasmid

#12730

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf42

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
pAcrF2

Plasmid

#89234

Purpose

Plasmid contains the gene for anti-CRISPR protein AcrF2 (gene 30 from bacteriophage D3112), with N-terminal 6his tag and TEV protease cleavage site

Depositor

Blake Wiedenheft

Article

Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

Insert

AcrF2

Tags

6xHis-TEV

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-Enhancer-ZC3H12D_PromoterWT

Plasmid

#153066

Purpose

ZC3H12D promoter region cloned upstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

ZC3H12D promoter region (ZC3H12D Human)

Use

Luciferase

Expression

Mammalian

Promoter

none

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pMDC123SB-OsMIR390-B/c

Plasmid

#61466

Purpose

Plant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.

Depositor

James Carrington

Article

Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1

Insert

OsMIR390-B/c

Expression

Plant

Mutation

O. sativa MIR390 precursor sequence including a c…

Promoter

2x35S

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf196

Plasmid

#12884

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf196

Expression

Mammalian

Available Since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
pSG5-ERRbeta-delta10

Plasmid

#52187

Purpose

Expresses human ERRbeta-delta10 splice variant in mammalian cells

Depositor

Rebecca Riggins

Article

Heckler et al Cell Cycle. 2014 Dec 12:0.

Insert

Estrogen-related receptor beta (ESRRB Human)

Expression

Mammalian

Mutation

wild type sequence; codon optimized to exclude Ec…

Promoter

SV40

Available Since

April 24, 2014

Availability

Academic Institutions and Nonprofits only
pKM60

Plasmid

#154159

Purpose

Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.

Depositor

Kevin Maringer

Article

Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

Insert

Firefly luciferase

Use

Luciferase

Mutation

4 wt NF-κB sites were replaced with Rel1A-specifi…

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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LB334 (Bcl-2 from -1287 to -1640)

Plasmid

#15241

Depositor

Linda Boxer

Article

Wilson et al Mol Cell Biol. 1996 Oct . 16(10):5546-56

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Bcl-2 fragment from -1287 to -1640 with P1

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
Clim-DC2-NMM-tdtomato

Plasmid

#129557

Purpose

pClimDC-NMM:tdT cell membrane marker

Depositor

Inaki Ruiz-Trillo

Article

Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

Insert

N-Myristoylation motif (NMM) fused to tdtomato

Expression

Bacterial

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf117

Plasmid

#12805

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf117

Expression

Mammalian

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pMP71-mtGFP-llo-lama4-alg8

Plasmid

#174605

Purpose

expresses palmytoylated GFP with a C terminal fusion of the LLO190 and minigenes of 2 neoantigens from the T3 tumor in genes Alg8 and Lama4

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

palmytoylGFP w/C-term fusion LLO190 and minigenes of 2 neoantigens from the T3 tumor in genes Alg8 Lama4

Expression

Mammalian

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PARL-FLAG-CT S65D+T69D+S70D

Plasmid

#13617

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 65, Thr 69 and Ser70 to Asp

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only