We narrowed to 14,814 results for: NTS;
-
Plasmid#176919Purposeexpress Granulin 1 without linker 2 in mammalian cellsDepositorInsertGranulin 1 (GRN Human)
TagsTwin-Strep and FLAG tags encoded after Signal Pep…ExpressionMammalianAvailable SinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_MAPK9-P2A-Hygro_Barcode
Plasmid#170238PurposeBarcoded lentiviral vector to express MAPK9 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX022
Plasmid#167148PurposeMoClo-compatible Level 0-CDS2 promoterless vector encoding C-terminal half of the Neonothopanus nambi hispidin synthase nnHispS codon-optimised for expression in Nicotiana benthamianaDepositorInsertC-part of fungal hispidin synthase, nnHispS
UseSynthetic BiologyAvailable SinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX021
Plasmid#167147PurposeMoClo-compatible Level 0-SP promoterless vector encoding N-terminal half of the Neonothopanus nambi hispidin synthase nnHispS codon-optimised for expression in Nicotiana benthamianaDepositorInsertN-part of fungal hispidin synthase, nnHispS
UseSynthetic BiologyAvailable SinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
p35S_EVDdi
Plasmid#167120PurposePlant binary expression vector containing the coding sequence of the Arabidopsis Copia93 retroelement EVADE (AT5G17125) lacking the intron under CaMV 35S promoter.DepositorInsertEVADE (EVD) (AT5G17125 Mustard Weed)
ExpressionPlantMutationDeleted intron from full length elementPromoterCaMV 35SAvailable SinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE P1214R
Plasmid#166810PurposeMammalian cell expression vector for FLAG-tagged NLRP1. LL1193EE and P1214R mutations abolish DPP9 association.DepositorAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE
Plasmid#166805PurposeMammalian cell expression vector for FLAG-tagged NLRP1. The LL1193E mutant abolishes FL-NLRP1 association with DPP9 at interface I.DepositorAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 LIC 6A NLRP1-FLAG K1277E
Plasmid#166808PurposeMammalian cell expression vector for FLAG-tagged NLRP1. The K1277E mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activityDepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 LIC 6A NLRP1-FLAG H1276G
Plasmid#166807PurposeMammalian cell expression vector for FLAG-tagged NLRP1. The H1276G mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activityDepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A FLAG-TEV-DPP9S RK96EE
Plasmid#166797PurposeMammalian cell expression vector for FLAG-tagged DPP9S. RK96EE mutant is partial loss of CARD8 binding.DepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A FLAG-TEV-DPP9S LL102EE
Plasmid#166799PurposeMammalian cell expression vector for FLAG-tagged DPP9S. LL102EE mutant is partial loss of CARD8 binding.DepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A FLAG-TEV-DPP9S D808N
Plasmid#166802PurposeMammalian cell expression vector for FLAG-tagged DPP9S. The D808N mutant is catalytically dead.DepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 LIC 6A NLRP1-FLAG P1214R
Plasmid#166806PurposeMammalian cell expression vector for FLAG-tagged NLRP1. The P1214R patient mutant abolishes NLRP1-CT association with DPP9 at interface II.DepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-MAVS-Gln93
Plasmid#158629PurposeGateway entry vector for an inducible 3xFLAG-tagged MAVS mutantDepositorAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha2 P35S:MS2-VP64:Tnos (GB1395)
Plasmid#160609PurposeTU for the constitutive expresion of the viral coat protein MS2 fused on C-terminal with the VP64DepositorInsertP35S:MS2-VP64:Tnos
ExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-MAVS-Ala148
Plasmid#158630PurposeGateway entry vector for an inducible 3xFLAG-tagged MAVS mutantDepositorAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A2-VN (G112P)
Plasmid#135496PurposeMammalian expression of VN-fused and myc-tagged HLA-A*02:01 (G112P mutant)DepositorInsertHLA-A*02:01 (HLA-A Human)
TagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationG112PPromoterCMVAvailable SinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A2-VN (L156P)
Plasmid#135497PurposeMammalian expression of VN-fused and myc-tagged HLA-A*02:01 (L156P mutant)DepositorInsertHLA-A*02:01 (HLA-A Human)
TagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationL156PPromoterCMVAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
B-HypaSpCas9
Plasmid#126763PurposeExpression plasmid for human codon-opt. increased fidelity Blackjack-HypaSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HypaSpCas9.DepositorInsertB-HypaSpCas9
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationN692A; M694A; Q695A; H698A; amino acids 1005-1013…PromoterCBhAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-FLAG-B-eSpCas9
Plasmid#126772PurposeExpression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9DepositorInsertB-eSpCas9
UseCRISPRTags3xFLAG, 6xHis, MBP, and NLSExpressionBacterialMutationK848A; K1003A; R1060A; amino acids 1005-1013 repl…PromoterT7Available SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only