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pEt28a-H6-SUMO-SilkCatcher

Plasmid

#230902

Purpose

Bacterial expression of SilkCatcher

Depositor

Sesilja Aranko

Article

Fan et al Angew Chem Int Ed Engl. 2023 Mar 6;62(11

Insert

H6-SUMO-SilkCatcher

Tags

HisTag, thrombin, SUMO

Expression

Bacterial

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pISEre1reRNA

Plasmid

#226821

Purpose

Expressing ISEre1 guide RNA targeting maeB site controled by PJ23119

Depositor

Sheng Yang

Article

Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

Insert

ISEre1 none target guide RNA

Expression

Bacterial

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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GB4003

Plasmid

#215248

Purpose

Module for the constitutive expression of PIF6 and PhyB:VP16

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

PIF6-PhyB:VP16

Use

Synthetic Biology

Mutation

V234L in PIF6- please see dep. sequence, BsaI and…

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX302_circSMAD2_OE

Plasmid

#215238

Purpose

Overexpression of pLEX302_circSMAD2(2,7)_OE expression

Depositor

Sefi Rosenbluh

Article

Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

Insert

circSMAD2

Use

Lentiviral

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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shRNA_LacZ

Plasmid

#215217

Purpose

Supression of shLacZ expression (Negative control)

Depositor

Sefi Rosenbluh

Article

Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

Insert

LacZ shRNA

Use

Lentiviral

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMZ103-rbs1-m1

Plasmid

#204463

Purpose

This is a biosensor with improved specificity to 3OC12-HSL, containing LasR A127N mutation.

Depositor

Lauren Andrews

Article

Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

Inserts

quorum sensing regulator LasR (lasR )
eYFP

Use

Synthetic Biology

Mutation

LasR A127N mutation

Promoter

In an operon and PLas

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pMZ103-rbs1-m2

Plasmid

#204464

Purpose

This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L128C mutation.

Depositor

Lauren Andrews

Article

Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

Inserts

quorum sensing regulator LasR (lasR )
eYFP

Use

Synthetic Biology

Mutation

LasR L128C mutation

Promoter

In an operon and PLas

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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GB4653

Plasmid

#215267

Purpose

TU for the constitutive expression of the flippase (FLP) recombinase with a nopaline synthase (Nos) promoter

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

pNos:FLP:tNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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GB4829

Plasmid

#215275

Purpose

TU for the constitutive expression of the N. nambi NPGA gene under the P35S promoter and the TNOS terminator

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

P35S:NPGA:TNOS

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV4.51-mut

Plasmid

#201060

Purpose

Expresses AAV2 Rep and AAV4.51 Cap

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV4.51 Cap

Use

AAV

Expression

Mammalian

Mutation

N586R, S587V, N588D, L589R

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV4.52-mut

Plasmid

#201061

Purpose

Expresses AAV2 Rep and AAV4.52 Cap

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV4.52 Cap

Use

AAV

Expression

Mammalian

Mutation

N586A, S587G, N588F, L589R

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV4.68-mut

Plasmid

#201064

Purpose

Expresses AAV2 Rep and AAV4.68 Cap

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV4.68 Cap

Use

AAV

Expression

Mammalian

Mutation

T591V, D593R, R594G, T596R

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV4.69-mut

Plasmid

#201065

Purpose

Expresses AAV2 Rep and AAV4.69 Cap

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV4.69 Cap

Use

AAV

Expression

Mammalian

Mutation

T591P, D593M, R594G, T596G

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pBsVchDonor

Plasmid

#203814

Purpose

DNA donor for transposition

Depositor

Sheng Yang

Article

Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

Insert

Mini-Tn, aprE cassette

Use

CRISPR

Expression

Bacterial

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW111112

Plasmid

#222457

Purpose

Level 1 acceptor, Position 3 containing CBS4-miniDFR::LacZ::nosT.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Mutation

no

Promoter

CBS4-miniDFR

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCASCADE-LCAmp-trxB

Plasmid

#202465

Purpose

Expresses a guide RNA to silence trxB after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

trxB guide RNA

Expression

Bacterial

Promoter

ugpB

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pRSET_SynPspA

Plasmid

#223812

Purpose

Bacterial expression of SynPspA with N-terminal 10xHis-tag and EK cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Cell. 2021 Jul 8;184(14):3674-3688.e18.

Insert

SynPspA

Tags

10xHis and NusA

Expression

Bacterial

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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GST-beta catenin tag

Plasmid

#186422

Purpose

Encodes GST fused to beta catenin tag recognized by anti-beta catenin nanobody

Depositor

Stephen Elledge

Article

Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

Insert

GST-beta catenin tag

Tags

6His and GST-beta catenin tag (PDRKAAVSHWQQRS)

Expression

Bacterial

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-UGCG-EGFP

Plasmid

#225878

Purpose

Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by EGFP insertion

Depositor

Yohei Sato

Article

Sato et al Biochem Biophys Res Commun. 2024 Sep 18;

Insert

EGFP

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET50delCT_Vipp1_dL10Ala

Plasmid

#223817

Purpose

Bacterial expression of Vipp1_dL10Ala with C-terminal 6x His-Tag and 3C cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Nat Struct Mol Biol. 2025 Mar;32(3):555-

Insert

Vipp1

Tags

6xHis

Expression

Bacterial

Mutation

replacement of 10 aa in Hinge 2 with Ala

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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