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pJAK184.tetR.PT5-3.dCas9

Plasmid

#232482

Purpose

optimized ahTet inducible dCas9 for insertion into C. difficile genome

Depositor

James Collins

Article

Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

Insert

Catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system

Use

CRISPR

Expression

Bacterial

Promoter

TetR-PT5-3

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-ER-KLF2-T2A-mCHERRY

Plasmid

#224297

Purpose

Expresses estrogen receptor linked KLF2 and mCherry under EF1a promoter

Depositor

Nikhil Joshi

Article

Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

Insert

Estrogen Receptor linked KLF2 T2A mCherry (Klf2 Mouse)

Use

Retroviral

Tags

Estrogen Receptor

Expression

Mammalian

Promoter

EF1a

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRP.ABCA4 c.5461-10T

Plasmid

#226553

Purpose

Minigene encoding the ABCA4 c.5461-10T mutation

Depositor

Dave Wee

Article

Tabaglio et al Mol Ther Nucleic Acids. 2025 Jan 7;36(1)

Insert

ABCA4 (ABCA4 Human)

Expression

Mammalian

Mutation

c.5461-10T intronic mutation

Promoter

CAG

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pRP.CEP290 c.2991+1655A>G

Plasmid

#226556

Purpose

Minigene encoding the CEP290 c.2991+1655A>G mutation

Depositor

Dave Wee

Article

Tabaglio et al Mol Ther Nucleic Acids. 2025 Jan 7;36(1)

Insert

CEP290 (CEP290 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

c.2991+1655A>G intronic mutation

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pGFP-CUG-IRES-mCherry

Plasmid

#222110

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Mutation

CUG start codon

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMT-C34-H

Plasmid

#223966

Purpose

Anti-MT-C34 heavy chain expression plasmid; to be used with the light chain plasmid, pMT-C34-L (Plasmid 223967), to make the antibody.

Depositor

Dmitriy Mazurov

Article

Kalinichenko et al Biology (Basel). 2024 Aug 29;13(9):675.

Insert

Variable domain of mouse anti-MT-C34 IgG1 heavy chain jointed to constant region of human IgG1 heavy chain

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2 EGFP sgRNA2

Plasmid

#164933

Purpose

Expresses EGFP sgRNA2 in mammalian cells

Depositor

Ji Luo

Article

Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

Insert

sgRNA2 targeting Enhanced green fluorescent protein (verified for knockout)

Use

Lentiviral

Promoter

EFS promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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eGFP Y93H AC editing reporter

Plasmid

#198883

Purpose

Express eGFP fluorescence after Y93H correction and mCherry as transfection control

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

enhanced green fluorescent protein

Use

Lentiviral

Expression

Mammalian

Mutation

Y93H, Gly92(GGT-to-GGA)

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4_1G11F2-3 heavy chain

Plasmid

#198253

Purpose

recombinant expression of heavy chain of monoclonal anti-AMP antibody 1G11F2-3

Depositor

Aymelt Itzen

Article

Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

Insert

heavy chain of monoclonal anti-AMP antibody 1G11F2-3 (mouse IgG2b)

Tags

IL10 signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-AcGFP-P2A-hBRD4S

Plasmid

#197882

Purpose

study roles of human BET proteins in human pluripotency reprogramming

Depositor

Kejin Hu

Article

Hossain et al iScience. 2022 Dec 28;26(1):105889. doi:

Insert

BRD4 (BRD4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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LentiU6-hASCL1 gRNA_multi1-3-MS2-Puro

Plasmid

#192682

Purpose

Lentiviral expression of multi gRNAs targeting hASCL1 promoter to activate human ASCL1 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human ASCL1 activating gRNAs #1,2,3 (ASCL1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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p20StAD

Plasmid

#190268

Purpose

Constitutively expresses a protein of interest fused to a GAL4 transcription activating domain. Targeted integration to genomic locus YPRC-delta15, Chromosome XVI.

Depositor

Assaf Mosquna

Article

Zimran et al ACS Synth Biol. 2022 Aug 19;11(8):2880-2

Type

Empty backbone

Use

Synthetic Biology; Yeast genome-integrating vector

Tags

GAL4-AD

Promoter

TDH3

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pPyCAG-HA-EGFP-Myc-TM-IRES-Pac

Plasmid

#183605

Purpose

Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the CAG promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

EGFP-TM

Tags

HA, Human PDGFRB transmembrane domain, Mouse IgGK…

Expression

Mammalian

Promoter

CAG

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]

Plasmid

#149426

Purpose

The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Inserts

U6.3-gRNAs[Sxl, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10

Use

CRISPR

Tags

eGFP

Expression

Insect

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pSPL3-ABCC8-9-10

Plasmid

#135918

Purpose

Encodes ABCC8 exons 9 and 10 wild type for the analysis of splicing variants

Depositor

Diana Valverde Pérez

Article

Lago-Docampo et al Sci Rep. 2020 Sep 15;10(1):15135. doi: 1

Insert

ATP-binding Cassette subfamily C member 8 (ABCC8 Human)

Use

Exon trapping

Expression

Mammalian

Promoter

SV40

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAT9751-BEAR-mCherry-preedited

Plasmid

#162993

Purpose

BEAR control plasmid with split mCherry and intact 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

mCherry split with an intron between amino acids 119-120

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pKB239

Plasmid

#170034

Purpose

Encodes LacI-controlled expression of surface-displayed GNLY and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
Granulysin (GNLY Synthetic, Human, Escherichia coli)

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-C1 SNX9 RYK mutant R286A Y287A K288A

Plasmid

#161993

Purpose

Mammalian expression vector containing a PIP-binding defective mutant of SNX9. Also contains mCherry tag

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

SNX9 (Snx9 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

R286A Y287A K288A

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pet-ECHB(35-475)

Plasmid

#96890

Purpose

expresses His-tagged mouse ECHB (35-475) in E.coli cells

Depositor

Hening Lin

Article

Sadhukhan et al Proc Natl Acad Sci U S A. 2016 Apr 19;11

Insert

Trifunctional enzyme subunit beta, mitochondrial (Hadhb Mouse)

Tags

His6 tag

Expression

Bacterial

Mutation

deleted amino acid 1-34

Promoter

T7

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A3-VN

Plasmid

#135506

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*03:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*03:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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