Addgene

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pCI Cas9 H2B-RFP

Plasmid

#92397

Purpose

CAG-driven ubiquitous expression of Cas9 with Histone2B-RFP reporter controlled by IRES

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-IRES-3xNLS-mScarlet-I3-pA (JDW 1360)

Plasmid

#224536

Purpose

A Gateway compatible 3' entry clone containing IRES-FLAG- NLS-mScarlet-I3

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

IRES-FLAG- NLS-mScarlet-I3

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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TUBG1

Plasmid

#87954

Purpose

expresses human gamma-tubulin with a GFP-tag on the N-terminal of the protein

Depositor

Maria Alvarado-Kristensson

Article

Rossello et al Heliyon. 2016 Sep 24;2(9):e00166. eColle

Insert

gamma-tubulin 1 (TUBG1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

whole protein, The C-terminal is a sh-gamma-tubul…

Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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opto-Cdc42

Plasmid

#174510

Purpose

Expresses opto-Cdc42 (mCherry-GGGSx2-Cdc42-GGGSx2-BcLOV4-GGGS-3xFLAG) in a pcDNA3.1 backbone.

Depositor

Brian Chow

Article

Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

Insert

opto-Cdc42-mCherry

Use

Tags

3xFLAG and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pME-mTurquoise-P2A-iCre (JDW 920)

Plasmid

#224518

Purpose

A Gateway compatible middle entry clone containing mTurquoise P2A iCre (codon optimized Cre)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

mTurquoise-P2A-iCre

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-GNB1

Plasmid

#133856

Purpose

Mammalian expression of GNB1 N-terminally tagged with EGFP

Depositor

Dave Piston

Article

Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

Insert

GNB1 (GNB1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Twin-Strep_IRES-EmGFP

Plasmid

#113884

Purpose

Mammalian (inducible) protein expression, bicistronic expression of cytosolic EmGFP

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Expression

Mutation

Promoter

pHR-CMV-TetO2

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCAX-EGFP-CIP4

Plasmid

#178363

Purpose

Expresses CIP4 c-terminal labeled with EGFP in mammalian cells

Depositor

Erik Dent

Article

Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

Insert

Cdc42 Interacting Protein 4 (TRIP10 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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AA331

Plasmid

#215954

Purpose

Fragmid fragment: (guide cassette) guide expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v2 [Sa]

Use

CRISPR; Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP Lamp1-Venus

Plasmid

#89937

Purpose

Expresses Lamp1 tagged with Venus in mammalian cells

Depositor

Noboru Mizushima

Article

Tsuboyama et al Science. 2016 Nov 25;354(6315):1036-1041

Insert

rat Lamp1 (Lamp1 Rat)

Use

Retroviral

Tags

Venus

Expression

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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PB-TetON-dual-SunTag-Hygro

Plasmid

#121119

Purpose

Endogenous gene activation with the SunTag CRISPR activation system

Depositor

Pablo Navarro

Article

Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

Insert

dCas9-GCN4x10_scFv-VP64

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Tet-ON

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCW57-GFP-miR-302cluster

Plasmid

#132549

Purpose

Doxycycline-inducible expression of human miR-302 cluster

Depositor

Tomáš Bárta

Article

Peskova et al Sci Rep. 2019 Oct 31;9(1):15759. doi: 10

Insert

human miR-302 cluster: MIR302B, MIR302C, MIR302A, MIR302D, MIR367

Use

Lentiviral; Doxycycline inducible, microrna expre…

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pCI H2B-GFP

Plasmid

#92399

Purpose

Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-EGFP reporter.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

IRES H2B EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_HA-BirA-ER

Plasmid

#113897

Purpose

Mammalian (inducible) expression of HA-tagged ER-resident E. coli biotin ligase

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

E. coli biotin ligase

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

CMV-MIE-TetO2

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pFLAG-Tetherin

Plasmid

#41070

Depositor

Paul Spearman

Article

Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

Insert

Tetherin (BST2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
mouse Drp1(K38A)

Plasmid

#26049

Depositor

David Chan

Article

Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

Insert

Drp1 (Dnm1l Mouse)

Use

Tags

Expression

Mammalian

Mutation

K38A GTPase mutant

Promoter

Available since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only
pEVOL-NBK-1

Plasmid

#207639

Purpose

A plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

Pyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)

Use

Tags

Expression

Bacterial

Mutation

The evolved synthetase NBK-1 contains mutations Y…

Promoter

araBAD

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-C-Solo-mStayGold-PXN

Plasmid

#227316

Purpose

Donor template for mStayGold insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

PXN Homology Arms flanking a mStayGold Tag (PXN Human)

Use

CRISPR; Donor template

Tags

Expression

Mammalian

Mutation

Promoter

Promoterless

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Rtn3LΔ6LIR-mNeonGreen

Plasmid

#169036

Purpose

Express human Rtn3L with 6x LC3-interacting regions mutated in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rtn3L (RTN3 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Changed following LC3-interacting regions of Rtn3…

Promoter

CMV

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Mito-mEosEM-E

Plasmid

#226335

Purpose

Photoswitchable fluorescent protein for correlative light-electron microscopy; mitochondria-targeted mEosEM-E for mammalian expression

Depositor

Pingyong Xu

Article

Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3

Insert

COX8A (COX8A Human)

Use

Tags

mEosEM-E

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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