We narrowed to 14,187 results for: shi

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Flag-Rabin8

Plasmid

#118070

Purpose

Expresses Flag-tagged human Rabin8 in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

human Rabin8 (RAB3IP Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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gh76

Plasmid

#106747

Purpose

expression of gRNA targeting MGAT1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

MGAT1 (MGAT1 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-Ms-c-Myc-W136E

Plasmid

#26024

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

c-Myc (Myc Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

W136E

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
pCFJ2334 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159850

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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p5079 pcDNA4c hBrd4 (731-1046)

Plasmid

#14455

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (731-1046) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 731-1046

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
BII-ChBtW-ASCL2

Plasmid

#133375

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

ASCL2 (ASCL2 Human)

Tags

Flag

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAV10.KH

Plasmid

#63208

Purpose

pCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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CoV2-N-R203K

Plasmid

#177951

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203K). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (R203K) (N )

Expression

Mammalian

Mutation

R203K

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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VPS4B, S2C

Plasmid

#184806

Purpose

Bacterial expression of VPS4B (S2C mutation)

Depositor

James Hurley

Article

Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

Insert

VPS4B (VPS4B Human)

Tags

His6

Expression

Bacterial

Mutation

S2C

Promoter

T7

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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TS MS-Sensor

Plasmid

#199788

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Article

Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

Insert

TS MS-Sensor

Use

Synthetic Biology

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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BII-ChiNW-ASCL2

Plasmid

#133384

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

ASCL2 (ASCL2 Human)

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pHLmMBP-2

Plasmid

#72344

Purpose

Mammalian expression of secreted N-terminally 6His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

Depositor

Luca Jovine

Article

Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

Type

Empty backbone

Tags

6His-tag, HA-tag, Strep-tag II, and mMBP

Expression

Mammalian

Promoter

CMV enhancer + chicken beta-actin promoter

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX Stat5A-SH2

Plasmid

#46515

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat5A (STAT5A Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 582-693

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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noSIIN

Plasmid

#185666

Purpose

shApc-expressing lentiviruses with no expression of SIINFEKL

Depositor

Tyler Jacks

Article

Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

Inserts

mScarlet
miR-30a
shApc

Use

Lentiviral

Promoter

Ef1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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p5071 pcDNA4c hBrd4 (595-1362)

Plasmid

#14450

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (595-1362) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 595-1362

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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AsCas12a-2C-NLS in pCSDest

Plasmid

#126637

Purpose

Expresses AsCas12a-2C-NLS in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

AsCas12a-2C-NLS

Use

CRISPR

Tags

3xHuman influenza hemagglutinin (HA)-Tag

Expression

Mammalian

Promoter

CMV IE94 promoter

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-LexA::GADfluw-Hsp70

Plasmid

#78304

Purpose

Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-nlsLexA::GADfluw-Hsp70

Available Since

June 2, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-GAL-Nat-ADE2

Plasmid

#232103

Purpose

Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Insert

ADE2 gRNA and repair template

Use

CRISPR

Expression

Yeast

Mutation

Repair template introduces C at nt 466 of ADE2 to…

Promoter

GAL7

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pX330.Pten.b

Plasmid

#66588

Purpose

sgRNA for Pten deletion

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

Pten deletion (Pten Mouse)

Expression

Mammalian

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
bArr2-ALFA

Plasmid

#201498

Purpose

Expression of the beta-arrestin2 containing a N-terminal ALFA tag.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

bArr2-ALFA (ARRB2 Human)

Tags

ALFA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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