We narrowed to 1,429 results for: tk

1. pPB-TRE-V5-TurboID-MUTT2A-SARS-CoV2-NCAP-bGH polyA > mPGK-BlastR-SV40 polyA

   Plasmid

   #218913

   Purpose

   PiggyBac transposition

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   V5-TurboID-MUTT2A-SARS-CoV2-NCAP/BlastR (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   2363G>C and 5335T>C

   Promoter

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-TRE-V5-TurboID-T2A-SARS-CoV2-NCAP-bGH polyA > mPGK-BlastR-SV40 polyA

   Plasmid

   #218912

   Purpose

   PiggyBac transposition

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   V5-TurboID-T2A-SARS-CoV2-NCAP/BlastR (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   2363G>C and 5335T>C

   Promoter

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pINSPECT:NEAT1-total_SigP:NLuc

   Plasmid

   #197267

   Purpose

   Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (both isoforms). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

   Insert

   NEAT1-total homology arms
   

   Use

   CRISPR, Luciferase, Synthetic Biology, and Unspec…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUt-mNF-GFP

   Plasmid

   #199220

   Purpose

   LPUtopia-7 matching RMCE donor plasmid with GFP Negative-Feedback circuit (mNF-GFP). Use BlastR for positive selection and HSV-TK for negative selection. 

   Depositor

   Gabor Balazsi

   Article

   Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

   Insert

   hTetR::P2A::EGFP
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV D2ir promoter

   Available Since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pINSPECT_SigP:NLuc

   Plasmid

   #197263

   Purpose

   Cloning Backbone for INSPECT expression. Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via Esp3I.

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

   Type

   Empty backbone

   Use

   CRISPR, Luciferase, Synthetic Biology, and Unspec…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MIGR1-Cdc42-1

   Plasmid

   #179517

   Purpose

   Retroviral expression of Cdc42 splice variant 1

   Depositor

   Bo Porse

   Article

   Rehn et al Cell Rep. 2022 May 10;39(6):110793. doi:

   Insert

   Cdc42 splice variant 1 cDNA (CDC42 Human)
   

   Use

   Retroviral

   Tags

   IRES-EGFP

   Expression

   Mutation

   Promoter

   LTR

   Available Since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MIGR1-Cdc42-2

   Plasmid

   #179518

   Purpose

   Retroviral expression of Cdc42 splice variant 2

   Depositor

   Bo Porse

   Article

   Rehn et al Cell Rep. 2022 May 10;39(6):110793. doi:

   Insert

   Cdc42 splice variant 2 cDNA (CDC42 Human)
   

   Use

   Retroviral

   Tags

   IRES-EGFP

   Expression

   Mutation

   Promoter

   LTR

   Available Since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223_CDC42_WT

   Plasmid

   #82104

   Purpose

   Gateway Donor vector containing CDC42 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   CDC42 (CDC42 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223_CDC42_p.Q61L

   Plasmid

   #82174

   Purpose

   Gateway Donor vector containing CDC42 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   CDC42 (CDC42 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Q61L

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUt-mNF-BACH1

    Plasmid

    #199219

    Purpose

    LPUtopia-7 matching RMCE donor plasmid with GFP-BACH1 Negative-Feedback circuit (mNF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 

    Depositor

    Gabor Balazsi

    Article

    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

    Insert

    hTetR::P2A::EGFP::BACH1 (BACH1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV D2ir promoter

    Available Since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNTK Gatad2a flox

    Plasmid

    #110812

    Purpose

    Donor targeting vector for generating mouse Gatad2a conditional knockout allele

    Depositor

    Jacob Hanna

    Article

    Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

    Insert

    mGataD2a (Gatad2a Mouse)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUt-mPF-BACH1 Cas9-Resistant_AmpR

    Plasmid

    #199221

    Purpose

    LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Positive-Feedback circuit (mPF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 

    Depositor

    Gabor Balazsi

    Article

    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

    Insert

    rtTA-Advanced::P2A::EGFP::BACH1 (BACH1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    silient mutation at nucleic acid 177 C changed to…

    Promoter

    tight TRE promoter

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUt-mNF-BACH1 Cas9-Resistant

    Plasmid

    #199218

    Purpose

    LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Negative-Feedback circuit (mNF-BACH1 Cas9- resistant). Use BlastR for positive selection and HSV-TK for negative selection. 

    Depositor

    Gabor Balazsi

    Article

    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

    Insert

    hTetR::P2A::EGFP::BACH1 (BACH1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    silient mutation at nucleic acid 177 C changed to…

    Promoter

    CMV D2ir promoter

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMaster1

    Plasmid

    #58525

    Purpose

    Reprogram somatic cells to iPS cells

    Depositor

    Mario Capecchi

    Article

    Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11

    Inserts

    neo
    tk
    oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 )
    myc (MYC Human)
    EBNA-1
    Amp
    Lin28 (LIN28A Human)
    Nanog (NANOG Human)
    IRES
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CAG and EF1a

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTKA001

    Plasmid

    #164577

    Purpose

    Expresses SARS-CoV-2 nsp7 from a pET46 backbone for IPTG-inducible bacterial expression

    Depositor

    Robert Kirchdoerfer

    Article

    Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

    Insert

    nsp7 (ORF1ab )
    

    Use

    Tags

    6xHis, Ek, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTKA002

    Plasmid

    #164578

    Purpose

    Expresses SARS-CoV-2 nsp8 from a pET46 backbone for IPTG-inducible bacterial expression

    Depositor

    Robert Kirchdoerfer

    Article

    Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

    Insert

    nsp8
    

    Use

    Tags

    6xHis, Ek, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMasterE

    Plasmid

    #165080

    Purpose

    Episomal expression of reprogramming factors.

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Stem Cell Res. 2021 May;53:102380. doi:

    Inserts

    OCT4 (POU5F1 Human)
    SOX2 (SOX2 Human)
    KLF4 (KLF4 Human)
    MYC (MYC Human)
    LRH1 (NR5A2 Human)
    NANOG (NANOG Human)
    LIN28 (LIN28A Human)
    EGFP
    HSV TK
    

    Use

    Episomal

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMaster3

    Plasmid

    #58526

    Purpose

    Reprogram somatic cells to iPS cells

    Depositor

    Mario Capecchi

    Article

    Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11

    Inserts

    neo
    tk
    oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 )
    myc (MYC Human)
    EBNA-1
    Amp
    Lin28 (LIN28A Human)
    Nanog (NANOG Human)
    IRES
    NR5A2 (NR5A2 Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMasterK

    Plasmid

    #165081

    Purpose

    Episomal expression of reprogramming factors.

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Stem Cell Res. 2021 May;53:102380. doi:

    Inserts

    OCT4 (POU5F1 Human)
    SOX2 (SOX2 Human)
    KLF4 (KLF4 Human)
    MYC (MYC Human)
    LRH1 (NR5A2 Human)
    NANOG (NANOG Human)
    lin28 (LIN28A Human)
    EGFP
    HSV TK
    KDM4D (KDM4D Callithrix jacchus)
    GLIS1 (GLIS1 Human)
    

    Use

    Episomal

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge