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We narrowed to 63,651 results for: cloning

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Showing: 941 - 960 of 63651 results

  2. NYESO beta/alpha into TRAC HDRT Source (pTR 169)

    Plasmid
    #112021
    Purpose
    DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha
    Depositor
    Alexander Marson
    Publication
    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NYESO beta/alpha into TRAC HDRT
    Use
    CRISPR and Synthetic Biology
    Available since
    Sept. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112021-plasmid-map-sequence-id-215596

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  3. lentiGuide-Hygro-mTagBFP2

    Plasmid
    #99374
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Publication
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-mTagBFP2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a and hU6
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99374-plasmid-map-sequence-id-430620

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  10. pCMV-MMLVgag-3xNES-ABE8e

    Plasmid
    #181751
    Purpose
    MMLV-Gag fused to 3xNES-ABE8e with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.
    Depositor
    David Liu
    Publication
    Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MMLVgag-3xNES-ABE8e
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    181751-plasmid-map-sequence-id-353935

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  11. CoV2-M-IRES-E

    Plasmid
    #177938
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Publication
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
    Expression
    Mammalian
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    177938-plasmid-map-sequence-id-429078

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  12. pDF0159 pCMV - huDisCas7-11 mammalian expression

    Plasmid
    #172507
    Purpose
    Encodes human codon optimized DisCas7-11 protein alone for expression in mammalian cells
    Depositor
    Omar Abudayyeh, Jonathan Gootenberg
    Publication
    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pCMV - huDisCas7-11 mammalian expression
    Expression
    Mammalian
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    172507-plasmid-map-sequence-id-344930

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Showing: 941 - 960 of 63651 results