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pLTR-G

Plasmid

#17532

Depositor

Jakob Reiser

Article

Reiser et al Proc Natl Acad Sci U S A. 1996 Dec 24. 9

Insert

VSV-G glycoprotein (Indiana strain)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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p5T7-LYCipi-ggpps

Plasmid

#122017

Purpose

Expresses genes for lycopene synthesis (crtI, crtB & ipi from Pantoea agglomerans, ggpps from T. canadiensis). IPTG inducible. Spec. resist.

Depositor

Gregory Stephanopoulos

Article

Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

Insert

GGPP synthase

Use

Tags

Expression

Bacterial

Mutation

codon optimized, truncated first 98 amino acids, …

Promoter

T7

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAC-U63-gRNA2.1

Plasmid

#170512

Purpose

gRNA cloning vector containing a U6:3 promoter and a gRNA2.1 scaffold.

Depositor

Chun Han

Article

Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

Type

Empty backbone

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

U6:3

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCFD2-dU6:2gRNA

Plasmid

#49409

Purpose

expression of gRNA under control of the Drosophila U6:2 promoter

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

dU6-2:gRNA

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

dU6-2

Available since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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RFP Dynamin2 K44A

Plasmid

#128153

Purpose

Expression of Dynamin 2 K44A

Depositor

Jennifer Lippincott-Schwartz

Article

Chua et al Proc Natl Acad Sci U S A. 2009 Nov 30. (

Insert

Dynamin 2 (Dnm2 Rat)

Use

Tags

Expression

Mammalian

Mutation

K44A

Promoter

Available since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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pTRE-Tight caspase-3 (p12)::nz [TU#817]

Plasmid

#16084

Depositor

Martin Chalfie

Article

Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

Insert

caspase-3 (p12) :: nz (CASP3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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pTRE-Tight cz::caspase-3 (p17) [TU#818]

Plasmid

#16085

Depositor

Martin Chalfie

Article

Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

Insert

cz :: caspase-3 (p17) (CASP3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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Phsp-16 cz::caspase-3 (p17) [TU#823]

Plasmid

#16086

Depositor

Martin Chalfie

Article

Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

Insert

cz :: caspase-3 (p17) (CASP3 Human)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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pCI-neo-sOVA

Plasmid

#25098

Depositor

Maria Castro

Article

Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

Insert

soluble ovalbumin

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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WT-eNOS-RFP

Plasmid

#22497

Depositor

William Sessa

Article

Iwakiri et al Proc Natl Acad Sci U S A. 2006 Dec 26. 1

Insert

WT eNOS

Use

Tags

RFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only
4xMS2-hTR

Plasmid

#154940

Purpose

4xMS2 stem-loop-hTR-500 bp genomic sequence

Depositor

Alice Ting

Article

Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

Insert

4xMS2 stem-loop-hTR-500 bp genomic sequence

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-HA-hTAF6

Plasmid

#17988

Depositor

Naoko Tanese

Article

Tanese et al Proc Natl Acad Sci U S A. 1996 Nov 26. 9

Insert

TAF6 (TAF6 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 19, 2008

Availability

Academic Institutions and Nonprofits only
tdTomato-Lifeact-7

Plasmid

#54528

Purpose

Localization: Actin, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

Type

Empty backbone

Use

Tags

tdTomato-Lifeact

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-PLE-IRES-mCherry

Plasmid

#45766

Depositor

Luke Lavis

Article

Tian et al Proc Natl Acad Sci U S A. 2012 Mar 27;10

Insert

esterase

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pWT PolI

Plasmid

#11721

Depositor

Lawrence Loeb

Article

Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

Insert

DNA polymerase I (polA E. coli)

Use

Tags

Expression

Bacterial

Mutation

wild-type

Promoter

Available since

Aug. 8, 2006

Availability

Academic Institutions and Nonprofits only

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AviTag-SpyCatcher

Plasmid

#72326

Purpose

Bacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

AviTag-SpyCatcher

Use

Tags

N-term His6 tag and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPE-600n

Plasmid

#13537

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E WT

Use

Tags

ECFP and Venus

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nmdCas9-3XGFP

Plasmid

#64109

Purpose

Expression of Nm dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Nm dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Mutation

Promoter

CMV-TO

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pSAC5.02

Plasmid

#16077

Depositor

Jochen Buck, Lonny Levin

Article

Buck et al Proc Natl Acad Sci U S A. 1999 Jan 5. 96

Insert

soluble adenylyl cyclase (Adcy10 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pVL1392 TMEM97

Plasmid

#157748

Purpose

Baculovirus transfer vectors to create a virus expressing human TMEM97

Depositor

Andrew Kruse

Article

Alon et al Proc Natl Acad Sci U S A. 2017 Jul 3;114

Insert

TMEM97 (TMEM97 Human)

Use

Tags

flag

Expression

Insect

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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