Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 30,217 results for: PLE;

Showing: 9621 - 9640 of 30217 results

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SMT503

Plasmid

#226840

Purpose

Plasmid that encodes a B. Subtilis lipase (BsLipA) gene fusion with N-terminal SNAP and C-Terminal eGFP under a T7 promoter. Compatible with PURExpress for in vitro transcription/translation. Chloramp

Depositor

Polly Fordyce

Article

Thompson et al Adv Mater Interfaces 2024, 12 (3), 24004

Insert

Lipase A

Use

Synthetic Biology

Tags

SNAP and eGFP

Expression

Bacterial

Mutation

R33Q, D34N, K35D, K112D, M134D, Y139C, I157M

Promoter

T7

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-SAP30

Plasmid

#109212

Purpose

Encodes human full-length SAP30 to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Jayaprakash et al Mol Biotechnol. 2021 Jan;63(1):53-62. do

Insert

Human full-length SAP30 (SAP30 Human)

Expression

Insect

Promoter

p10

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-aptamer

Plasmid

#236373

Purpose

crRNA array expression

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

Aptamer array

Use

CRISPR and Lentiviral

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pKG3777_pGEEC584_pShip-EFS(promoter)-CMV(UTR)-mRuby2-bGH

Plasmid

#239746

Purpose

Plasmid expressing mRuby2 from the EFS canonical promoter with the CMV 5' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pKG3668_pGEEC578_pShip-EFS-rtTA-P2A-iRFP720-bGH

Plasmid

#239741

Purpose

Plasmid expressing a doxycycline-inducible transcriptional activator

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

rtTA-P2A-iRFP720

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG3831_pGEEC579_pShip-UbC(no_intron)-mRuby2-bGH

Plasmid

#239742

Purpose

Plasmid expressing mRuby from a UbC promoter lacking an intron, used for modRNA production

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG3774_pGEEC580_pShip-EF1a(no_intron)-mRuby2-bGH

Plasmid

#239743

Purpose

Plasmid expressing mRuby from an EF1a promoter lacking an intron, used for modRNA production

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG3775_pGEEC582_pShip-hPGK(promoter)-CMV(UTR)-mRuby2-bGH

Plasmid

#239744

Purpose

Plasmid expressing mRuby2 from the hPGK canonical promoter with the CMV 5' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG3776_pGEEC583_pShip-hPGK(promoter)-EFS(UTR)-mRuby2-bGH

Plasmid

#239745

Purpose

Plasmid expressing mRuby2 from the hPGK canonical promoter with the EFS 5' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG3778_pGEEC585_pShip-EFS(promoter)-hPGK(UTR)-mRuby2-bGH

Plasmid

#239747

Purpose

Plasmid expressing mRuby2 from the EFS canonical promoter with the hPGK 5' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG3779_pGEEC586_pShip-CMV(promoter)-EFS(UTR)-mRuby2-bGH

Plasmid

#239748

Purpose

Plasmid expressing mRuby2 from the CMV canonical promoter with the EFS 5' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG3780_pGEEC587_pShip-CMV(promoter)-hPGK(UTR)-mRuby2-bGH

Plasmid

#239749

Purpose

Plasmid expressing mRuby2 from the CMV canonical promoter with the hPGK 5' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2436_pGEEC532_pShip-EF1a-mRuby2-P2A-PuroR-bGH

Plasmid

#239728

Purpose

Plasmid expressing mRuby2 and PuroR with the EF1a promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2437_pGEEC533_pShip-EFS-mRuby2-P2A-PuroR-bGH

Plasmid

#239729

Purpose

Plasmid expressing mRuby2 and PuroR with the EFS promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2438_pGEEC534_pShip-CMV-mRuby2-P2A-PuroR-bGH

Plasmid

#239730

Purpose

Plasmid expressing mRuby2 and PuroR with the CMV promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2439_pGEEC535_pShip-hPGK-mRuby2-P2A-PuroR-bGH

Plasmid

#239731

Purpose

Plasmid expressing mRuby2 and PuroR with the hPGK promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2440_pGEEC536_pShip-UbC-mRuby2-P2A-PuroR-bGH

Plasmid

#239732

Purpose

Plasmid expressing mRuby2 and PuroR with the UbC promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2441_pGEEC537_pShip-CAG-mRuby2-P2A-PuroR-bGH

Plasmid

#239733

Purpose

Plasmid expressing mRuby2 and PuroR with the CAG promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG3660_pGEEC570_pShip-ZF10.BSx8-ybTATA-spacer-mRuby2-bGH

Plasmid

#239739

Purpose

Plasmid expressing mRuby2 from a SynZiFTR promoter, Induced with pKG2309

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2309_pGEEC524_pShip-EFS-ZF10-NS3-p65-bGH

Plasmid

#239722

Purpose

Plasmid expressing a grazoprevir-inducible transcriptional activator

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

ZF10-NS3-p65

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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