We narrowed to 1,131 results for: Ncl

1. pAc5.1B-EGFP-DmAGO2-RC_B

   Plasmid

   #145980

   Purpose

   Insect Expression of DmAGO2-RC

   Depositor

   Elisa Izaurralde

   Article

   Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

   Insert

   DmAGO2-RC (AGO2 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   A deletion of AA 51-56 and an insertion of 23 AA …

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. VHACg/pDONR221

   Plasmid

   #172094

   Purpose

   Genomic fragment containing the upstream region and coding region of DET3/VHA-C cloned into pDONR221

   Depositor

   George Haughn

   Article

   Shi et al Plant Physiol. 2019 Nov;181(3):901-915.

   Insert

   VHA-C genomic region including 530bp upstream of the ATG, exons, introns, and no stop codon (DET3 Mustard Weed)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   530bp upstream of VHA-C ATG

   Available since

   Oct. 20, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pHH0103_DYNLL2_Dynein-light

   Plasmid

   #110005

   Purpose

   Protein expression and purification of DYNLL2_Dynein-light

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   DYNLL2_Dynein-light (DYNLL2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-Miro1 (412-618)

   Plasmid

   #127628

   Purpose

   GTPase2 domain of human Miro1 including transmembrane domain and C-terminal amino acids

   Depositor

   Josef Kittler

   Article

   Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

   Insert

   Miro1 (RHOT1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   412-618 only

   Promoter

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. UASp-deltaN cycB

   Plasmid

   #112967

   Purpose

   P element vector for transposon insertion of nondegradable Cyclin B into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Article

   Lee et al Genetics. 2001 Aug;158(4):1545-56.

   Insert

   cycB (CycB Fly)
   

   Use

   P insertion vector

   Tags

   Expression

   Mutation

   N-term deletion of first 46 amino acids, includes…

   Promoter

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR CMVie-Lifeact-EGFP L1-R5

   Plasmid

   #84391

   Purpose

   Multisite Gateway entry vector for expression of EGFP-tagged Lifeact in cells including neurons

   Depositor

   Ghassan Mouneimne

   Article

   Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

   Insert

   Lifeact
   

   Use

   Gateway

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV immediate early

   Available since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. EF.delmFasL.CMV.GFP

   Plasmid

   #17620

   Depositor

   Linzhao Cheng

   Article

   Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

   Insert

   delFasL (Fasl Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Noncleavable mouse delFasL . Deleted the exon 2 r…

   Promoter

   Available since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAcGP67A-mMD2-proA

   Plasmid

   #187878

   Purpose

   baculovirus protein expression of mouse MD-2 (residues 19–160) fused to a protein A tag

   Depositor

   Bruce Beutler

   Article

   Wang et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

   Insert

   MD2 (Ly96 Mouse)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   includes residues 19–160 of mouse MD-2

   Promoter

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. MBP-CHC(1-1074)-His6

   Plasmid

   #100748

   Purpose

   Bacterial expression of MBP-tagged clathrin heavy chain large fragment including N-terminal domain, his tagged at C-terminus for purification.

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   CLTC (CLTC Human)
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   Residues 1-1074

   Promoter

   Available since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. GG-EBNA-MIR302-7guides-PGK-Puro

    Plasmid

    #201960

    Purpose

    EBNA episome plasmid for U6 promoter-driven expression of 7 gRNAs targeting miRNA302/367. Includes PGK-puro selection cassette

    Depositor

    Timo Otonkoski

    Article

    Sokka et al Stem Cell Reports. 2022 Feb 8;17(2):413-

    Insert

    MIR302-7g-PGK-Puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

    Plasmid

    #154473

    Purpose

    Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ZIM3 (ZIM3 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Includes ZIM3 aa 1-100

    Promoter

    SFFV

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. PMmEos3.2

    Plasmid

    #203755

    Purpose

    Encodes the membrane anchor of Lyn including the first 10 residues of the N terminus, with 1 myristoylation and 1 palmitoylation, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    Lyn n-term (LYN Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. FKBP-LBR(1-245)-GFP

    Plasmid

    #222425

    Purpose

    To express a truncated LBR (encoding amino acids 1-245) with N-terminal FKBP tag accessible to the cytoplasm and C-terminal GFP in mammalian cells

    Depositor

    Stephen Royle

    Article

    Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

    Insert

    LBR(1-245aa) (LBR Human)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    Truncation (Includes aa 1-245)

    Promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-HDR-mEGFP-Actin

    Plasmid

    #119870

    Purpose

    AAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse Beta Actin

    Depositor

    Ryohei Yasuda

    Article

    Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

    Insert

    beta Actin (Actb Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSicoR-Ef1a-mCh-Puro

    Plasmid

    #31845

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression. Includes puromycin selection.

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry-puromycin

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. FLAG-LoxP-GFP-LoxP-SNAP-TERT

    Plasmid

    #71391

    Purpose

    Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes GFP marker flanked by LoxP sites for selection.

    Depositor

    Thomas Cech

    Article

    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

    Insert

    TERT (TERT Human)
    

    Use

    Hr donor

    Tags

    FLAG-SNAP

    Expression

    Mutation

    Promoter

    Endogenous TERT promotor

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGImC_V5-CSNK1A1

    Plasmid

    #92014

    Purpose

    Expresses V5-CSNK1A1, includes IRES-mCherry

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    CSNK1A1 (CSNK1A1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-HDR-mEGFP-camk2a

    Plasmid

    #104589

    Purpose

    AAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse camk2a

    Depositor

    Ryohei Yasuda

    Article

    Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

    Insert

    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MmEos3.2

    Plasmid

    #203754

    Purpose

    Encodes the membrane anchor of Src including the first 15 resideues of the N terminus, with 1 myristoylation and short polybasic sequence, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    Src15 n-term (SRC Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBetaActin-FKBP-mScarlet-Dync1h1motor

    Plasmid

    #191330

    Purpose

    Expresses the fluorescently labeled dynein motor domain fused to dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    FKBP-mScarlet-Dync1h1motor (Dync1h1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    DYNC1H1 motor domain aa 1453-4644

    Promoter

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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