We narrowed to 4,230 results for: Pet2

1. pET21d-myc-BirA

   Plasmid

   #109424

   Purpose

   Bacterial expression of biotin ligase (BirA) with an N-terminal myc epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   BirA (birA )
   

   Use

   Tags

   myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET22b-CPDBamHI-Leu

   Plasmid

   #38256

   Depositor

   Aimee Shen, Matthew Bogyo

   Article

   Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

   Type

   Empty backbone

   Use

   Tags

   6xHis and Vibrio cholerae MARTX toxin cysteine pr…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET22b-TEVP-cleavableELP

   Plasmid

   #219402

   Purpose

   Tobacco etch virus protease-responsive elastin-like polypeptide (ELP)

   Depositor

   Danielle Mai

   Article

   Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

   Insert

   TEVP-cleavableELP
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-MH6-RspWYL1

   Plasmid

   #108306

   Purpose

   Expresses E. coli codon optimized RspWYL1 in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

   Insert

   RspWYL1
   

   Use

   CRISPR

   Tags

   mH6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET29b Tau Cys

   Plasmid

   #108867

   Purpose

   Expresses human Tau with a mutated cysteine

   Depositor

   Gabriele Kaminski Schierle

   Insert

   Tau (MAPT Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   I260C C291A C322A to allow maleimide dye labelling

   Promoter

   T7

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET23b-Casp9-His

   Plasmid

   #11829

   Depositor

   Guy Salvesen

   Article

   Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-6

   Insert

   Caspase 9 (CASP9 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

7. hPU.1ΔN165-pET28b

   Plasmid

   #199796

   Purpose

   Expressed human PU.1/Spi-1 ETS domain (residues 165 to 270) in E. coli

   Depositor

   Gregory Poon

   Article

   Xhani et al Sci Adv. 2020 Feb 21;6(8):eaay3178. doi:

   Insert

   spi1 (SPI1 Human)
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28_6xHis-PARP1-E988K

   Plasmid

   #173945

   Purpose

   Bacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-E988K (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   V762A, E988K

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28_human-pro-IL18

   Plasmid

   #183390

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   pro-IL18 (IL18 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-SUMO-DogTag

    Plasmid

    #105629

    Purpose

    Plasmid for recombinant expression in bacteria of SUMO domain fused to DogTag, permitting covalent linkage to SnoopTagJr

    Depositor

    Mark Howarth

    Article

    Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

    Insert

    SUMO-DogTag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET23b-Casp3-His

    Plasmid

    #11821

    Depositor

    Guy Salvesen

    Article

    Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-SUMO-MjDim1

    Plasmid

    #189936

    Purpose

    Expression of Mjdim1, a methyltransferase used in m6A-SAC-seq.

    Depositor

    Chuan He

    Article

    Hu et al Nat Biotechnol. 2022 Mar 14. pii: 10.103

    Insert

    rsmA (MJ_RS00455 Methanocaldococcus jannaschii)
    

    Use

    Tags

    6x His tag, FLAG tag, SUMO tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28b-hUba2-His

    Plasmid

    #53117

    Purpose

    expression of full length human Uba2 with Cterminal His-tag in E.coli

    Depositor

    Frauke Melchior

    Article

    Werner et al Methods Mol Biol. 2009;497:187-99. doi:

    Insert

    UBA2 (UBA2 Human)
    

    Use

    Tags

    His and T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21 gamma-tubulin

    Plasmid

    #101825

    Purpose

    production of gamma-tubulin in bacteria

    Depositor

    Maria Alvarado-Kristensson, Ana Carrera

    Article

    Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

    Insert

    gamma-tubulin 1 (TUBG1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Whole protein

    Promoter

    T7

    Available since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21b-Casp1-His

    Plasmid

    #11809

    Depositor

    Guy Salvesen

    Insert

    Caspase-1 (CASP1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET23a-H6-TEV-cSrc

    Plasmid

    #214233

    Purpose

    Bacterial expression of the kinase domain of cSrc with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings

    Depositor

    Neel Shah

    Article

    Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 1

    Insert

    cSrc Kinase domain (SRC Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21a Ce-iPGM-bio

    Plasmid

    #162559

    Purpose

    Expresses C. elegans iPGM containing a C-terminal biotinylation site and His tag

    Depositor

    James Inglese

    Article

    Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

    Insert

    C. elegans iPGM bio-6His (ipgm-1 Nematode)
    

    Use

    Tags

    6His tag, T7 tag, Thrombin cleavage site, and bio…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21a Bm-iPGM-bio

    Plasmid

    #162565

    Purpose

    Expresses B. malayi iPGM containing a C-terminal biotinylation site and His tag

    Depositor

    James Inglese

    Article

    Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

    Insert

    B. malayi iPGM bio-6His
    

    Use

    Tags

    6His tag, T7 tag, biotinylation sequence, and thr…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21-pelB-LaG-16

    Plasmid

    #172746

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-16
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET24b-SUMO-BG2.4

    Plasmid

    #199438

    Purpose

    expresses BAmGreen2.4 in bacterial cells with a SUMO tag

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    BAMGreen2.4
    

    Use

    Tags

    his tag and sumo

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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