We narrowed to 10,452 results for: T7

1. pcDNA-HIRA-polyA

   Plasmid

   #59781

   Purpose

   Express H3.3 chaperone HIRA in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   HIRA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-EGFP-ELYS-polyA

   Plasmid

   #59746

   Purpose

   Express EGFP-ELYS in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   EGFP-ELYS
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA-emerin-EGFP-polyA

   Plasmid

   #59747

   Purpose

   Express emerin-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   emerin-EGFP
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pet28a-ClbB-NRPS-NHis

   Plasmid

   #49217

   Purpose

   NRPS module of ClbB containing CAT domains with N-terminal 6His tag

   Depositor

   Emily Balskus

   Article

   Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

   Insert

   ClbB-NRPS
   

   Use

   Tags

   6x His tag and T7 tag

   Expression

   Bacterial

   Mutation

   contains aa4-1080 of reference sequence NP_754362…

   Promoter

   T7

   Available since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. mt TEFM

   Plasmid

   #205058

   Purpose

   TEFM protein expression

   Depositor

   Smita Patel

   Article

   Sultana et al J Biol Chem. 2017 Nov 3;292(44):18145-18

   Insert

   TEFM (TEFM Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized

   Promoter

   Available since

   Feb. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDB023f1

   Plasmid

   #99218

   Purpose

   Complementary plasmid for aaRS PACE; amber codons in T7 RNAP

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Ppsp [SD4] T7RNAP(S12*,S203*)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   amber codons at positions 12 and 203 of T7 RNA po…

   Promoter

   Ppsp

   Available since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET23a-Mm/HsUBA52

   Plasmid

   #69560

   Purpose

   Bacterial expression; in vitro transcription/translation (T7)

   Depositor

   Jorge Eduardo Azevedo

   Article

   Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

   Insert

   UBA52 (UBA52 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA-Flag-RCC1-polyA

   Plasmid

   #59762

   Purpose

   Express Flag-tagged RCC1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   RCC1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTHSSd_27

   Plasmid

   #59958

   Purpose

   PTac null fragment (sigma CGG Y639A) expression

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

   Insert

   null fragment
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   PTac

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDB023f

    Plasmid

    #99217

    Purpose

    Complementary plasmid for aaRS PACE; amber codons in T7 RNAP

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] T7RNAP(S12*,S203*)
    

    Use

    Tags

    Expression

    Mutation

    amber codons at positions 12 and 203 of T7 RNA po…

    Promoter

    Ppsp

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. bbCas9pluspAAA

    Plasmid

    #82581

    Purpose

    The plasmid encodes the T7 promoter, Cas9 (from pX330 #42230) and a stretch of poly-A. After linearization with restriction enzyme SapI It is used to produce in vitro transcribed mRNA

    Depositor

    Ivo Huijbers

    Article

    Pritchard et al Methods Mol Biol. 2017;1642:21-35. doi:

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDB023k

    Plasmid

    #99219

    Purpose

    Complementary plasmid for aaRS PACE; amber codons in T7 RNAP

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] T7RNAP(S12*,S203*,S527*)
    

    Use

    Tags

    Expression

    Mutation

    amber codons at positions 12, 203, and 527 of T7 …

    Promoter

    Ppsp

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTHSSd_38

    Plasmid

    #59961

    Purpose

    High core fragment expression (high resource allocator)

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

    Insert

    core fragment
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    PJ23105

    Available since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTHSSd_1

    Plasmid

    #59947

    Purpose

    Splitposon in KanR ColE1 backbone

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

    Insert

    Splitposon w/ KanR, LacI & PTac
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-ClbN-NHis

    Plasmid

    #51497

    Purpose

    NRPS module containing CATE domains with N-terminal 6His tag

    Depositor

    Emily Balskus

    Article

    Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

    Insert

    ClbN
    

    Use

    Tags

    6x His tag and T7 tag

    Expression

    Bacterial

    Mutation

    contains aa30-1094 of NP_754347.1

    Promoter

    T7

    Available since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET23a-MmUBC

    Plasmid

    #69555

    Purpose

    Bacterial expression; in vitro transcription/translation (T7)

    Depositor

    Jorge Eduardo Azevedo

    Article

    Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

    Insert

    UBC (Ubc Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pARiBo1

    Plasmid

    #98892

    Purpose

    Expresses ARiBo-fused RNA in vitro with T7 RNA polymerase

    Depositor

    Pascale Legault

    Article

    Di Tomasso et al Nucleic Acids Res. 2011 Feb;39(3):e18. d

    Insert

    ARiBo1
    

    Use

    In vitro transcription vector

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET23a-MmUBB

    Plasmid

    #69556

    Purpose

    Bacterial expression; in vitro transcription/translation (T7)

    Depositor

    Jorge Eduardo Azevedo

    Article

    Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

    Insert

    UBB (Ubb Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pTHSSd_39

    Plasmid

    #59962

    Purpose

    Low core fragment expression (low resource allocator)

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

    Insert

    core fragment
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    PJ23105

    Available since

    Dec. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTHSSd_40

    Plasmid

    #59963

    Purpose

    High core fragment expression without SynZIP

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

    Insert

    core fragment lacking SynZIP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    PJ23105

    Available since

    Oct. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge