We narrowed to 79,895 results for: TRI

1. Ratiometric FPX sensor for ERK kinase activity

   Plasmid

   #60974

   Purpose

   For imaging of ERK kinase activity using a single polypeptide RA-WW domain-ERK substrate-B protein and free GA-NES.

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddRFP A-WW domain-ERK substrate-ddFP B
   

   Use

   Tags

   ERK substrate is before ddFP copy B, WW domain is…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pUC19_T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP

   Plasmid

   #195503

   Purpose

   SOX17 donor plasmid to generate locus specific C-terminal integration of H2B-mCitrine into the human SOX17-locus

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   SOX17(Ex2)-T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP_SOX17(3'UTR) (SOX17 Human)
   

   Use

   CRISPR; Donor vector

   Tags

   T2A-H2B-mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-DIO-HA-rM3D(Gs)-IRES-mCitrine

   Plasmid

   #50456

   Purpose

   Double floxed Gs-coupled rM3D-IRES-mCitrine under the control of human synapsin promoter

   Depositor

   Bryan Roth

   Insert

   rM3D-IRES-mCitrine (CHRM3 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   human Synapsin 1

   Available since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRIP-SFFV-mtagBFP-2A STING V155M 368STOP

   Plasmid

   #102596

   Purpose

   Lentivector to express STING V155M delta 368 and mTagBFP

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   V155M delta 368

   Promoter

   SFFV

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRIP-SFFV-mtagBFP-2A STING-V155M S366A

   Plasmid

   #102593

   Purpose

   Lentivector to express STING V155M S366A and mTagBFP

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   V155M S366A

   Promoter

   SFFV

   Available since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMOS018E: Entry vector for: ratiometric pHluorin (mitchondrial)

   Plasmid

   #163070

   Purpose

   Entry vector for: ratiometric pHluorin (mitchondrial)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   ratiometric pHluorin
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. FRP791_insul-(lexA-box)1-PminCYC1-Citrine-TCYC1

   Plasmid

   #58432

   Purpose

   Induces Citrine expression from a promoter containing 1 lexA box in yeast cells

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   CitrineA206K
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   insul-(lexA-box)1-PminCYC1

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. Type I inositol-1,4,5-trisphosphate 5-phosphatase in pcDNA3

   Plasmid

   #121976

   Purpose

   transfection studies in eukaryotic cells such as COS-7 cells

   Depositor

   Christophe Erneux

   Article

   De Smedt et al J Biol Chem. 1996 Apr 26;271(17):10419-2

   Insert

   Type I Inositol-1,4,5-trisphoosphate 5-phosphatase (INPP5A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC029 - Lw2Cas13a from Leptotrichia wadei F0279

   Plasmid

   #91919

   Purpose

   Expresses Lw2Cas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   Lw2Cas13a and guide expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRRL-5xTetO-pEF-H2B-Citrine-SV40

    Plasmid

    #186966

    Purpose

    Lentiviral vector encoding fluorescent reporter (5xTetO-pEF-H2B-Citrine-SV40) for expression in mammalian cells.

    Depositor

    Brian Liau

    Article

    Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

    Insert

    5xTetO-pEF-H2B-Citrine-SV40
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5xTetO-pEF-1alpha

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTRIP TRE-BI GFP10-RHOB/RBD-GFP11

    Plasmid

    #182231

    Purpose

    Inducible coexpression of GFP10-RHOB and RBD-GFP11 domain

    Depositor

    Stéphanie Cabantous

    Article

    Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

    Inserts

    ras homolog family member B (RHOB Human)
    rhotekin RHO binding domain (RBD)
    

    Use

    Lentiviral

    Tags

    GFP10 (split-GFP tripartite) and GFP11 (M4)

    Expression

    Mutation

    Promoter

    TRE bidirectional

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Equalizer-L (mScarlet-I) plasmid with onboard mCitrine cassette

    Plasmid

    #169737

    Purpose

    Equalizer-L plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    miR(FF4) target-tetR-P2A-mScarletI-miR(FF4) target-miR(FF4)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV-tetO2

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG APX W41F dimeric (mito matrix)

    Plasmid

    #40307

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

    Insert

    pea APX W41F (dimeric)
    

    Use

    Tags

    Flag

    Expression

    Mutation

    W41F mutation.

    Promoter

    CAG

    Available since

    Oct. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. Triple ORF LoxP ifgMosaic Donor (LH208)

    Plasmid

    #99625

    Purpose

    To clone the 3 ORFs for Cre dependent generation of ifgMosaics

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    N-PhiM
    

    Use

    Cre/Lox, Mouse Targeting, and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMOS017E: Entry vector for: ratiometric pHluorin (cytosolic)

    Plasmid

    #163069

    Purpose

    Entry vector for: ratiometric pHluorin (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    ratiometric pHluorin
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Flag-TRIM24-C840W/F869D/F979A/N980A

    Plasmid

    #28144

    Depositor

    Michelle Barton

    Insert

    TRIM24 (TRIM24 Human)
    

    Use

    Tags

    FLAGX2

    Expression

    Mammalian

    Mutation

    C840W, F869D, F979A, N980A

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

17. FRP800_insul-(lexA-box)3-PminCYC1-Citrine-TCYC1

    Plasmid

    #58436

    Purpose

    Induces Citrine expression from a promoter containing 3 lexA boxes in yeast cells

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    CitrineA206K
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    insul-(lexA-box)3-PminCYC1

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTRIP-SFFV-mtagBFP-2A STING V155M 342STOP

    Plasmid

    #102594

    Purpose

    Lentivector to express STING V155M delta 342 and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    V155M delta 342

    Promoter

    SFFV

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. PHY98-LMNA 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

    Plasmid

    #164046

    Purpose

    CRISPR donor plasmid to insert TriTag (mTagBFP) into the N-terminus of human LMNA gene

    Depositor

    Baohui Chen

    Article

    Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

    Insert

    mTagBFG-TriTag
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA-mRIPK3 K51A-Flag/cHA-pTRIPZ vector

    Plasmid

    #73704

    Purpose

    lentiviral expression of mRIPK3 mutant

    Depositor

    Francis Chan

    Insert

    RIPK3 (Ripk3 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    K51A

    Promoter

    CMV/tet

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only