We narrowed to 14,477 results for: NTS

1. pX458_mCherry-HP1a-gRNA-2

   Plasmid

   #237635

   Purpose

   Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to HP1a locus

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   HP1a gRNA (CBX5 Human)
   

   Use

   CRISPR

   Tags

   mCherry

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5_mEGFP-SS18-SSX1-KS

   Plasmid

   #237679

   Purpose

   For overexpression of mEGFP-SS18-SSX1-KS

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   SS18-SSX1-KS (SS18, SSX1 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5_mEGFP-SS18-SSX1

   Plasmid

   #237678

   Purpose

   For overexpression of mEGFP-SS18-SSX1

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   SS18-SSX1 (SS18, SSX1 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5_mEGFP-SS18-SSX2-KS

   Plasmid

   #237680

   Purpose

   For overexpression of mEGFP-SS18-SSX2-KS

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   SS18-SSX2-KS (SS18, SSX2 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX458_mCherry-HP1a-gRNA-1

   Plasmid

   #237634

   Purpose

   Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to HP1a locus

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   HP1a gRNA (CBX5 Human)
   

   Use

   CRISPR

   Tags

   mCherry

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX458_mCherry-TCOF1-gRNA

   Plasmid

   #237633

   Purpose

   Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to TCOF1 locus

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   TCOF1 gRNA (TCOF1 Human)
   

   Use

   CRISPR

   Tags

   mCherry

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUC19_msfGFP-FUS-repair-tempate

   Plasmid

   #237684

   Purpose

   For knock-in msfGFP to FUS locus

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   FUS (FUS Human)
   

   Use

   CRISPR

   Tags

   msfGFP

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAK-DR30(SapI)-hfCas13d-Puro-pA/EF1A-mCherry-WPRE-pA

   Plasmid

   #233046

   Purpose

   To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

   Insert

   RfxCas13d-compatible gRNA expression cassette and hfCas13d and mCherry
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAK-DR30(SapI)-CasRx-Puro-pA/EF1A-mCherry-WPRE-pA

   Plasmid

   #233045

   Purpose

   To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

   Insert

   RfxCas13d-compatible gRNA expression cassette and CasRx and mCherry
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AID-C12*-n'Cas9-BE4max

    Plasmid

    #216730

    Purpose

    Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and n'Cas9 (Cas9 with H840A mutation) in mammalian cells.

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

    Insert

    AID-C12*-n'Cas9-BE4max (AICDA Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. AP2u2-CMV-Ace2-IgG1

    Plasmid

    #226455

    Purpose

    Mammalian expression of human Ace2 protein under CMV promoter fused with IgG1, tagged with 6His, and released extracellular environment after expression for protein purification

    Depositor

    Urartu Seker

    Article

    Ozcelik et al ACS Pharmacol Transl Sci. 2024 Mar 21;7(

    Inserts

    Angiotensin Converting Enzyme 2
    Immunoglobulin Heavy Constant Gamma 1
    

    Tags

    6xHis Tag

    Expression

    Mammalian

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Rab11 single guide RNA

    Plasmid

    #229683

    Purpose

    guide RNA for Rab11 tagging at the N-terminus in pX459

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    Rab11 guide (RAB11A Human)
    

    Use

    CRISPR

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. MAC-C Sars-CoV-2 S_L241-243del

    Plasmid

    #194841

    Purpose

    Sars-CoV-2 S_L241-243del in MAC-C destination vector

    Depositor

    Markku Varjosalo

    Article

    Varjosalo et al 10 May 2024, Research Square

    Insert

    S_L241-243del (S Sars-CoV-2)
    

    Tags

    MAC-C

    Expression

    Mammalian

    Mutation

    L241-243del

    Available Since

    May 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. PX458-3xHA-dFnCas9

    Plasmid

    #201954

    Purpose

    Mammalian expression plasmid of dead FnCas9 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    3xHA-NLS-dFnCas9-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D11A and H969A on FnCas9

    Promoter

    Cbh

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-His6-en1dFnCas9GFP

    Plasmid

    #201950

    Purpose

    Expression of en1dFnCas9-GFP in bacterial cells

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    Expression of en1dFnCas9-GFP in bacterial cells
    

    Use

    CRISPR

    Tags

    6x His, HA, NLS, and mEGFP

    Expression

    Bacterial

    Mutation

    E1369R on FnCas9

    Promoter

    T7

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-His6-en15dFnCas9GFP

    Plasmid

    #201951

    Purpose

    Expression of en15dFnCas9-GFP in bacterial cells

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    Expression of en15dFnCas9-GFP in bacterial cells
    

    Use

    CRISPR

    Tags

    6x His, HA, NLS, and mEGFP

    Expression

    Bacterial

    Mutation

    E1603H on FnCas9

    Promoter

    T7

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. PX458-3xHA-dSpCas9

    Plasmid

    #201953

    Purpose

    Mammalian expression plasmid of dead SpCas9 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    3xHA-NLS-dSpCas9-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A and H840A on SpCas9

    Promoter

    Cbh

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJT350 Lenti pEF-dCas9-3XFLAG-MBDa-T2A-tagBFP-P2A-Blast

    Plasmid

    #187347

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-MBDa-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJT351 Lenti pEF-dCas9-3XFLAG-P66_CC-T2A-tagBFP-P2A-Blast

    Plasmid

    #187348

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-P66_CC-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJT256 Lenti pEF-dCas9-3XFLAG-N+F-T2A-tagBFP-P2A-Blast

    Plasmid

    #187332

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-N+F-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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