163,354 results

1. pATetO 6XHis T4 DNA Ligase

   Plasmid

   #206207

   Purpose

   Inducible expression of T4 DNA ligase

   Depositor

   Andrew Ellington

   Article

   Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

   Insert

   T4 DNA ligase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK-ATF4

   Plasmid

   #26114

   Depositor

   Yihong Ye

   Article

   Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

   Insert

   ATF4 (ATF4 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV[Exp]-Puro-EF-1α>{H2B-RFP}

   Plasmid

   #201121

   Purpose

   RFP expression in cells under EF-1α promoter; cytoplasmic and histone-bound, nuclear

   Depositor

   Ori Bar-Nur

   Article

   Zvick et al Stem Cell Reports. 2022 Sep 13;17(9):194

   Insert

   H2B-RFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   No

   Promoter

   Available since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOT_7-lenti-EFS-SS1-BBz-2A-puro

   Plasmid

   #217990

   Purpose

   Expresses anti-Mesothelin CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Xue et al bioRxiv 2024.11.27.625752

   Insert

   SS1-BBz CAR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGIPZ-PD-L1-EGFP

   Plasmid

   #120933

   Purpose

   Express PD-L1-EGFP fusion protein in mammalian cells

   Depositor

   Mien-Chie Hung

   Article

   Yang et al Cell Res. 2018 Aug;28(8):862-864. doi: 1

   Insert

   PD-L1-EGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBR BsmAIM Kan + p15A BsaIR Amp

   Plasmid

   #206208

   Purpose

   Contains two plasmids. Constitutive expression of BsmAIM and inducible expression of BsaIR

   Depositor

   Andrew Ellington

   Article

   Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

   Insert

   BsamAIM and BsaIR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTriEx4-Cdc42-2G

   Plasmid

   #68814

   Purpose

   FRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)

   Depositor

   Olivier Pertz

   Article

   Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

   Insert

   Cdc42 activation reporter
   

   Use

   Tags

   His and Myc

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. p6XHis_NLS-SaCas9

   Plasmid

   #101086

   Purpose

   Express SaCas9 in bacteria with a 6xHis tag for purification

   Depositor

   Rick Tarleton

   Article

   Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

   Insert

   CRISPR-associated protein Cas9/Csn1
   

   Use

   CRISPR

   Tags

   TrxA-6xHis-STag

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJL046

   Plasmid

   #198807

   Purpose

   In vivo neuronal inhibition through histamine-gated chloride channel. Neurons expressing HisCl1 transgene are inhibited after addition of histamine.

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::HisCl1-SL2-GFP::let-858 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. EC70191

    Plasmid

    #209452

    Purpose

    Cloning protospacers for Cas9 editing

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas9 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    HvU3

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMuLE Lenti Dest eGFP

    Plasmid

    #62175

    Purpose

    MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses EGFP under the control of a PGK promoter. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    Lentiviral; Mule gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTF-ahpC-rfp

    Plasmid

    #153035

    Purpose

    For CRISPR-Cas12a genome editing in E. coli. Contains a constituitively expressed CRISPR array targeting the ahpC gene in E. coli and donor DNA to insert an rfp translational fusion in the ahpC gene.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    CRISPR array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CAG-PinkyCaMP

    Plasmid

    #232860

    Purpose

    An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under CAG promoter

    Depositor

    Olivia Masseck

    Article

    Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

    Insert

    PinkyCaMP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF0740

    Plasmid

    #141678

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    JDP2 (JDP2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBMN YFP-FKBP(DD)

    Plasmid

    #31766

    Depositor

    Thomas Wandless

    Article

    Chu et al Bioorg Med Chem Lett. 2008 Nov 15;18(22)

    Insert

    HA-YFP-FKBP(DD) (FKBP1A Human)
    

    Use

    Retroviral

    Tags

    HA and YFP

    Expression

    Mutation

    E31G, F36V, R71G and K105E on FKBP12

    Promoter

    LTR

    Available since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. TALED_Left-ND1-E1347A

    Plasmid

    #183895

    Purpose

    Expresses TALED_Left-ND1-E1347A in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    TALED for ND1 mutation
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5-FRT-TetO-U2B''-fSNAPc

    Plasmid

    #106988

    Purpose

    open reading frame of U2B'' inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

    Depositor

    Melissa Moore

    Article

    Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

    Insert

    UB2" (SNRPB2 Human)
    

    Use

    Tags

    fSNAP

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1a-CCreV

    Plasmid

    #140132

    Purpose

    can be used to generate AAV virus that will express fusion protein of split Cre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

    Depositor

    Hongkui Zeng

    Article

    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

    Insert

    CCreV
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EF1a-NCreV

    Plasmid

    #140131

    Purpose

    can be used to generate AAV virus that will express fusion protein of split Cre (N-terminal) and fungal photoreceptor Vivid (VVD) monomer

    Depositor

    Hongkui Zeng

    Article

    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

    Insert

    NCreV
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEUI(+)

    Plasmid

    #111834

    Purpose

    For mamalian expression of Ecdysone receptor-based inducible transgene expression switch.

    Depositor

    Hyunju Ro

    Article

    Lee et al Mol Ther Nucleic Acids. 2016 Sep 27;5(9)

    Insert

    GAL4-minimal VP16-EcR (EcR Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SV40, 10XUAS

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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