We narrowed to 22,456 results for: his
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Plasmid#167354PurposeExpression of human RNF216 (Triad3A) codon optimized for E. coli and insect cellsDepositorInsertE3 ubiquitin-protein ligase RNF216 (RNF216 Human)
Tags3C protease cleavage site and 6x-His tagExpressionBacterialMutationcodon optimised for expression in E. coliPromoterT7Available SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Akt-STOPS
Plasmid#175006PurposeAkt Substrate-based Tandem Occupancy Peptide Sponge. Genetically encoded for perturbing cellular Akt kinase activity.DepositorInsertmCherry-Akt-STOPS
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Akt-Scramble
Plasmid#175014PurposeNegative-control construct for Akt-STOPS.DepositorInsertmCherry-Akt-Scramble
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDmAct5C::Cas9-2A-Neo
Plasmid#176679PurposeExpression of Drosophila codon optimized spCas9 under Drosophila Act5C promoterDepositorInsertspCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
UseCrisprExpressionInsectMutationDrosophila codon optimizedPromoterD. melanogaster Act5CAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nsp3 Mac1 - P43 (Oxford)
Plasmid#169210PurposeExpression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removalDepositorInsertSARS-CoV-2 Nsp3 Mac1
Tags6xHisExpressionBacterialPromoterT7Available SinceJuly 28, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a Gx-mNG-NL
Plasmid#169106PurposeExpresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - mNeonGreen - NanoLuc
Tagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available SinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_NSP3
Plasmid#169378PurposeCell-free/mammalian expression vector for SARS-COV-2 virus NSP3 with N-term eGFPDepositorInsertSARS-COV-2 _NSP3
UseCell-free expression vectorTagsHis and eGFPPromoterT7- CMV/CAGAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8m-p10
Plasmid#162384PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8m
Tags6xHisExpressionInsectMutationA25G F286YPromoterhsp70Available SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
O-TREM2-2
Plasmid#166545PurposescFv of a human scaffold targeting Triggering receptor expressed on myeloid cells 2. Antigen coverage aa 19-131 of 230DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIA1362
Plasmid#166860PurposeExpression of codon optimized SARS-COV-2 Nsp7 in E. coliDepositorAvailable SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS/D614G
Plasmid#164566PurposeSARS-CoV-2 Spike protein with D614G mutation (S-GSAS-D614G variant)DepositorInsertSpike (S-GSAS-D614G variant)
Tags2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…ExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…Available SinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWB501NL3F10H
Plasmid#141286PurposeNo promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, HygR for plant)DepositorTypeEmpty backboneUseGateway Cloning and LuciferaseTagsFLAG (x3), HIS (x10), and NLUCExpressionPlantAvailable SinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
DT044A
Plasmid#159405PurposeInducible Perfringolysin O (PFO) expression and purification, controlled under hybrid IPTG-regulated T7/LacO promoter (pRT30)DepositorInsertpr.T7-LacO-6xHis-PFO (Pfo, Clostridium perfringens)
UseSynthetic BiologyTags6xHisExpressionBacterialPromoterT7/LacO promoterAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TGFα-GSY-SPM
Plasmid#163615PurposeExpresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.DepositorAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBTSG
Plasmid#159420PurposeTransient expression of proteins with a C-terminal thermostable GFP (TGP) tag in mammalian cellsDepositorInsertthermostable GFP (TGP)
Tagspolyhistidine (poly-His)ExpressionMammalianAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETSG
Plasmid#159418PurposeExpression of proteins with a C-terminal thermostable GFP (TGP) tag in Escherichia coliDepositorInsertthermostable GFP (TGP)
Tagspolyhistidine (poly-His)ExpressionBacterialAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
ClCry4 PHR
Plasmid#160552PurposeExpression of protein for various experiments including finding interaction partnersDepositorInsertClcry4 HR
TagsHexa hisExpressionInsectAvailable SinceNov. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDest-635
Plasmid#159582PurposeGateway Destination vector for protein expression in baculovirusDepositorTypeEmpty backboneTagsHis6ExpressionInsectAvailable SinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMCRW1
Plasmid#122462PurposeHuman NPM-ALK fusion protein gene in pET28a bacterial expression plasmid. Production of recombinant human NPM-ALK fusion protein in E. coli Bl21 Rosetta2 cellsDepositorTagsHis TagExpressionBacterialPromoterT7lac promoterAvailable SinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL.EED
Plasmid#125174PurposeExpresses human EED in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.DepositorAvailable SinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only