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pCAG PSAM4-5HT3 HC IRES EGFP

Plasmid

#119740

Purpose

Chemogenetic activator expression plasmid

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Insert

PSAM4 a75HT3 HC (homo) IRES eGFP (CHRNA7, HTR3A Synthetic, Human)

Use

Tags

Expression

Mammalian

Mutation

L131G, Q139L, Y217F, R420Q, R424D, R428A

Promoter

CAG

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-TVA-P2A-EGFP-2A-oG

Plasmid

#85225

Purpose

Expresses TVA, EGFP, and optimized Rabies G (oG) protein in a FLEX cassette

Depositor

John Naughton

Inserts

Optimized G Protein
Enhanced GFP
TVA (CD320 Chicken)

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

hSyn

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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Halo-Sec31

Plasmid

#166895

Purpose

mammalian expression of Sec31 tagged with HaloTag

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

Sec31

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV-EF1aGFP2AKAT7(E508Q)-W

Plasmid

#159293

Purpose

Lentiviral vector expressing GFP and KAT7 (gRNA-resistant, E508Q mutant) driven by human EF1a promoter

Depositor

Kosuke Yusa

Article

Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

Insert

KAT7 E508Q

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

CRISPR sites mutated (gRNA ID. 5 and A10) and E50…

Promoter

human EF1a promoter

Available since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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ALKBH5-dCas9

Plasmid

#134783

Purpose

Expression fusion protein ALKBH5-dCas9 in Mammalian cells

Depositor

Shu-Bing Qian

Article

Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

Insert

ALKBH5 and inactive dCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1

Plasmid

#222994

Purpose

Mammalian expression of iGeoCas9(C2) construct with EGFP-targeting sgRNA

Depositor

Jennifer Doudna

Article

Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

Insert

HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1

Use

CRISPR

Tags

Puro

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGAS-HA

Plasmid

#130910

Purpose

Expresses human cGAS-HA; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

cGAS

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_01-mEGFP

Plasmid

#194681

Purpose

CMV driven expression of the calcium recorder Caprola_01 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_01-mEGFP

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pKL06

Plasmid

#104430

Purpose

super Ecliptic pHluorin with C terminal mRuby2 fusion tag in yeast expression vector

Depositor

Joel Kralj

Article

Dodd et al Sci Rep. 2017 Oct 24;7(1):13922. doi: 10

Insert

Super Ecliptic pHluorin-mRuby2

Use

Tags

Expression

Yeast

Mutation

Promoter

Tef1

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCW57.1-DUX4-WT

Plasmid

#99282

Purpose

All-in-one lentivector with tet-inducible DUX4 (wildetype nucleotide sequence)

Depositor

Stephen Tapscott

Article

Jagannathan et al Hum Mol Genet. 2016 Oct 15;25(20):4419-4

Insert

DUX4 (DUX4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 iCasper T2A HO1

Plasmid

#64278

Purpose

Expresses infrared fluorescent executioner caspase reporter (iCasper) plus human heme oxygenase 1

Depositor

Xiaokun Shu

Article

To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

Infrared fluorescent executioner caspase reporter (iCasper) T2A heme oxygenase-1: (HMOX1 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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014LP-pAAV-ANF-657- Cre-pA

Plasmid

#120279

Purpose

AAV vector for Atrial specific Cre recombinase expression

Depositor

Xander Wehrens

Article

Ni et al Circ Res. 2019 Jan 18;124(2):256-262. do

Insert

Cre

Use

AAV

Tags

CRE

Expression

Mutation

Promoter

ANF promoter

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pRRL-SV40-Puro-CMV-mCherry-EGFP-LC3B

Plasmid

#223712

Purpose

Expresses autophagy flux LC3B fluorescence reporter

Depositor

Benjamin Wolozin

Article

Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

Insert

LC3B (MAP1LC3B Synthetic, Human)

Use

Lentiviral and Synthetic Biology

Tags

mCherry::EGFP::LC3B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pv6_CBX1_2xChromo

Plasmid

#179393

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX1_2xChromo

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-tdTomato-P2A-BlasR (LRT2B)

Plasmid

#110854

Purpose

Lentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markers

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

sgRNA scaffold with spacer

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

U6

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-MetGFP

Plasmid

#37560

Depositor

David Rimm

Insert

MET (MET Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pMBIS

Plasmid

#17817

Depositor

Jay Keasling

Article

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 27, 2008

Availability

Academic Institutions and Nonprofits only

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rTetR-GFP

Plasmid

#183916

Purpose

Reverse Tet repressor labeled with GFP; binds tetO sites upon doxycycline addition

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

rTetR-GFP

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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CRBN_ΔHBD

Plasmid

#232779

Purpose

Expresses CRBN_ΔHBD sequence in E. coli cells with cleavable MBP-His tag and deleted internal DDB1 binding domain

Depositor

Ivan Dikic

Article

Bailey et al Cell Chem Biol. 2024 Nov 20:S2451-9456(2

Insert

Cereblon (CRBN Human)

Use

Tags

MBP-His6-TEV

Expression

Bacterial

Mutation

CRBN (residues 47 to 193 and 249 to 436) with GNG…

Promoter

T7

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF0037

Plasmid

#141488

Purpose

Lentiviral vector for overexpressing the SP5 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SP5 (SP5 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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