161,186 results

1. pcDNA3.1-Flag-NPM1wt

   Plasmid

   #131810

   Purpose

   Expresses Flag-NPM1wildtype (human) in mammalian cells through transient transfection

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   NPM1 (NPM1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmScarlet3-Giantin_C1

   Plasmid

   #189773

   Purpose

   Construct for labelling Golgi apparatus in mammalian cells using mScarlet3-Giantin fusion

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet3-Giantin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AnkyrinB-2XHA

   Plasmid

   #31057

   Depositor

   Vann Bennett

   Article

   Ayalon et al J Biol Chem. 2011 Mar 4. 286(9):7370-8.

   Insert

   Ankyrin B (ANK2 Human)
   

   Use

   Tags

   2XHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLV-neo-CD2-FAK

   Plasmid

   #37013

   Depositor

   Bob Weinberg

   Article

   Shibue et al Proc Natl Acad Sci U S A. 2009 Jun 23;10

   Insert

   CD2-FAK (CD2, PTK2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZa-tetO-csgA-CmR

   Plasmid

   #89579

   Purpose

   Express csgA curli protein

   Depositor

   Urartu Seker

   Article

   Abdelwahab et al Langmuir. 2017 Apr 7. doi: 10.1021/acs.l

   Insert

   csgA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRX-IPU-GFP-MYH9

   Plasmid

   #168273

   Purpose

   Expresses MYH9 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

   Insert

   MYH9 (MYH9 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDD112

   Plasmid

   #119893

   Purpose

   pNG2 backbone with an optimized chloramphenicol resitance marker for Rhodococcus opacus PD630

   Depositor

   Tae Seok Moon

   Article

   DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEMT-3P2A

   Plasmid

   #111772

   Purpose

   Quadcistronic construct: Co-express four genes by 3 P2A peptides

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Type

   Empty backbone

   Use

   Cloning intermediate

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-DIO-rM3D(Gs)-mCherry

   Plasmid

   #50458

   Purpose

   Double floxed Gs-coupled rM3D DREADD fused with mCherry under the control of human synapsin promoter

   Depositor

   Bryan Roth

   Has Service

   AAV8

   Insert

   rM3D-mCherry
   

   Use

   AAV

   Tags

   HA and mCherry

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   human Synapsin 1

   Available since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKM596

    Plasmid

    #8837

    Depositor

    David Waugh

    Article

    Fox et al FEBS Lett. 2003 Feb 27. 537(1-3):53-7.

    Type

    Empty backbone

    Use

    Tags

    E. coli MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5FRT-EF-Pdgfralpha-EGFPN

    Plasmid

    #66787

    Purpose

    Expression of murine PDGFRalpha tagged with GFP under the control of EF1a promoter

    Depositor

    Lotte Pedersen

    Article

    Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

    Insert

    PDGFRalpha (Pdgfra Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. NW11

    Plasmid

    #133443

    Purpose

    Covalently circularized nanodisc NW11 for studying membrane proteins and viral entry.

    Depositor

    Gerhard Wagner

    Article

    Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

    Insert

    NW11
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV_hSyn1-SIO-stGtACR2-FusionRed

    Plasmid

    #105677

    Purpose

    Expresses a soma-targeted GtACR2 in Cre-expressing neurons

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

    Has Service

    AAV Retrograde, AAV1, and AAV5

    Insert

    stGtACR2
    

    Use

    AAV

    Tags

    FusionRed and Kv2.1

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-Neo-Bam p53 V143A

    Plasmid

    #16435

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 V143A (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V143A. Mutant cDNA from human colorectal tumor CX…

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pAW-NbVHH05-GFP

    Plasmid

    #171570

    Purpose

    Expresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cells

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    NbVHH05-GFP (UBE2J1 Human)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    fly actin5C promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Prab-3::NLS::GFP

    Plasmid

    #68120

    Purpose

    Nuclear Localized GFP for pan-neuronal expression in C. Elegans

    Depositor

    Andrew Leifer

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

    Insert

    GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rab-3

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-PL

    Plasmid

    #20783

    Depositor

    Bryan Cullen

    Article

    Zeng et al Mol Cell. 2002 Jun . 9(6):1327-33.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

18. mem-mCherry-pTRIP

    Plasmid

    #163521

    Purpose

    Used to construct lentivirus to express membrane-targeted mCherry in mammalian cells

    Depositor

    Thomas Weber

    Insert

    membrane targeted mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTRIPZ-p21-HA(RxL)

    Plasmid

    #215111

    Purpose

    Expresses p21-HA(RxL) in mammalian cells from an inducible lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    p21(RxL) denotes mutations: R19A, R20A, L21A, F22…

    Promoter

    CMV (Tet inducible)

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX-4T MDM2 WT

    Plasmid

    #16237

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge