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Addgene
Showing: 81 - 100 of 1730 results
  1. pUA97

    Plasmid
    #69340
    Purpose
    It encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategy
    Depositor
    Insert
    ceBFP SEC 3xFlag
    Use
    Tags
    3xFlag and ceBFP (mTagBFP)
    Expression
    Worm
    Mutation
    sqt-1(e1350)
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. pJW1328

    Plasmid
    #69488
    Purpose
    sgRNA(F+E) targeting pha-1 (no Cas9); for co-conversion in C. elegans
    Depositor
    Insert
    sgRNA(F+E) targeting pha-1
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    R07E5.16 U6 promoter
    Availability
    Academic Institutions and Nonprofits only
  3. pKPY737

    Plasmid
    #72673
    Purpose
    Encodes Thr412Gly-CePheRS Alpha Subunit (Gateway Entry Clone)
    Depositor
    Insert
    Thr412Gly-CePheRS Alpha Subunit::C. elegans CEOP5428 Intercistronic Region::HA-GFP [From pPD95.75 (+118 to +1000)] in pDONR221
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Thr412Gly in CePheRS Alpha Subunit
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. pKPY738

    Plasmid
    #72674
    Purpose
    C. elegans let-858 3' UTR (Gateway Entry Clone)
    Depositor
    Insert
    let-858 3' UTR [From pPD129.57 (+2554 to +2960)] in pDONR P2R-P3
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. pDD286 (TagRFP^SEC^3xMyc)

    Plasmid
    #70684
    Purpose
    TagRFP-T^SEC^3xMyc vector with ccdB sites for cloning homology arms.
    Depositor
    Insert
    TagRFP-T-C1^SEC^3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xMyc and C. elegans codon-optimized TagRFP-T
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  6. pSA120

    Plasmid
    #59789
    Purpose
    C. elegans heat-shock driven ZIF-1 and mCherry co-expressed in the same operon
    Depositor
    Inserts
    hsp-16.41 promoter
    zif-1 coding
    gpd-2/gpd-3 intergenic sequence
    mCherry (worm)
    unc-54 3' UTR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  7. pJN601

    Plasmid
    #59790
    Purpose
    zf1::yfp fusion with floxed unc-119(+) within an intron of yfp. For creating zf1::yfp knock-ins using unc-119 as a marker
    Depositor
    Inserts
    ZF1 domain from pie-1
    N-terminal half of yfp with introns
    intron containing floxed unc-119 gene in reverse orientation
    C-terminal half of yfp with introns
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 81 - 100 of 1730 results