We narrowed to 11,879 results for: pcDNA

1. pCDNA3_LuxSit_i_mTagBFP2

   Plasmid

   #194300

   Purpose

   Mammalian expression of the de novo luciferase, LuxSit-i

   Depositor

   David Baker, Andy Yeh

   Article

   Yeh et al Nature. 2023 Feb;614(7949):774-780. doi:

   Insert

   LuxSit-i
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.3_SARS2_JN.1

   Plasmid

   #218678

   Purpose

   expressing SARS-CoV-2 JN.1 spike protein for pseudovirus production

   Depositor

   David Nemazee

   Insert

   Spike (JN.1)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L455S on the basis of BA.2.86

   Promoter

   CMV

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1_MMP-9-HA

   Plasmid

   #121172

   Purpose

   Expression of mouse MMP9

   Depositor

   Shinwu Jeong, M. Elizabeth Fini

   Article

   Jeong et al Am J Pathol. 2012 May;180(5):2028-39. do

   Insert

   MMP9 (matrix metallopeptidase 9) (Mmp9 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   CMV immediate/early

   Available since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCDNA_GFP_hTLN2-FL

   Plasmid

   #164840

   Purpose

   Expression of GFP tagged FL human talin2

   Depositor

   Tina Izard

   Article

   Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

   Insert

   TLN2 (TLN2 Human)
   

   Use

   Tags

   His10-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDNA_MET_D14_3Flag

   Plasmid

   #182495

   Purpose

   Contains cDNA of the MET gene without the exon 14

   Depositor

   Paolo Comoglio

   Article

   Cerqua et al Life Sci Alliance. 2022 May 30;5(10). pi

   Insert

   MET (MET Human)
   

   Use

   Tags

   C-terminal 3xFlag tag

   Expression

   Mammalian

   Mutation

   Exon 14 deleted

   Promoter

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3_erGAP3

   Plasmid

   #78118

   Purpose

   Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum

   Depositor

   Javier García-Sancho, Teresa Alonso

   Article

   Navas-Navarro et al Cell Chem Biol. 2016 Jun 1. pii: S2451-9

   Insert

   erGAP3
   

   Use

   Tags

   KDEL and calreticulin signal peptide

   Expression

   Mammalian

   Mutation

   S175G, D180Y, I167V and L15Q in GFP moiety and D…

   Promoter

   CMV

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDNA3.1_WiChR_TS_mScarlet_ER

   Plasmid

   #195190

   Purpose

   High efficient, K+ selective channelrhodopsin for the inhibition of excitable cells

   Depositor

   Peter Hegemann, Johannes Vierock

   Article

   Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

   Insert

   Wobblia lunata inhibitory Channelrhodopsin
   

   Use

   Tags

   ER-export sequence, Kir2.1 trafficking motif, and…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDNA3.0_mitoSNAP

   Plasmid

   #136623

   Purpose

   Plasmid encoding outer mitochondrial localization SNAP-Tag

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   NTOM20-SNAP-Tag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1_PsChR2_EYFP

   Plasmid

   #69057

   Purpose

   High-efficiency blue-shifted cation channelrhodopsin

   Depositor

   John Spudich

   Article

   Govorunova et al J Biol Chem. 2013 Oct 11;288(41):29911-2

   Insert

   PsChR2
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.3_SARS2_XBB

    Plasmid

    #194494

    Purpose

    expressing SARS-CoV-2 XBB spike protein for pseudovirus production

    Depositor

    David Nemazee

    Article

    Zhou et al Immunity. 2023 Mar 14;56(3):669-686.e7.

    Insert

    Spike (XBB) (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V83A, Y144-,H146Q, Q183E, V213E, G339H, L368I, R3…

    Promoter

    CMV

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HcKCR2_mCherry_pcDNA3.1

    Plasmid

    #177337

    Purpose

    Expresses KCR2 in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

    Insert

    HcKCR2
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    mammalian codon-adapted

    Promoter

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5FRT_KCNQ3

    Plasmid

    #173163

    Purpose

    To generate Flp-In CHO-KCNQ3 stable cells

    Depositor

    Al George

    Article

    Vanoye et al JCI Insight. 2022 Mar 8;7(5):e156314. do

    Insert

    KCNQ3 (KCNQ3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5_FRT_mCH

    Plasmid

    #127109

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA4_ChR2opt_mKATE

    Plasmid

    #67958

    Purpose

    optical depolarization of mammalian cells

    Depositor

    Andreas Offenhäusser

    Insert

    Channelrhodopsin 2 opt
    

    Use

    Tags

    mKatushka

    Expression

    Mammalian

    Mutation

    synthetically produced for mammalian optimized co…

    Promoter

    CMV -TetON

    Available since

    Sept. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1_ionRD

    Plasmid

    #172931

    Purpose

    Ionic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.

    Depositor

    Arnold Boersma

    Article

    Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

    Insert

    IonicStrengthSensorRD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNAFlagMenin WT

    Plasmid

    #32079

    Depositor

    Matthew Meyerson

    Article

    Francis et al PLoS One. 2011 . 6(1):e16119.

    Insert

    Menin
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.3_mCherry

    Plasmid

    #26823

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Sep 29. ():.

    Insert

    5'UTR-mCherry-3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pcDNAKv10.1mVenus

    Plasmid

    #85705

    Purpose

    Kv10.1 fused mVenus

    Depositor

    Luis Pardo

    Article

    Chen et al PLoS One. 2011 May 3;6(5):e19257. doi: 1

    Insert

    KCNH1 (KCNH1 Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-HA-14-3-3 ϵ

    Plasmid

    #48797

    Purpose

    expresses human myc-14-3-3ϵ in mammalian cells

    Depositor

    Huda Zoghbi

    Article

    Chen et al Cell. 2003 May 16;113(4):457-68.

    Insert

    YWHAE 14-3-3 ϵ (YWHAE Human)
    

    Use

    Tags

    haemagglutinin

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.3_SARS2_BA.4/5

    Plasmid

    #186810

    Purpose

    pseudovirus plasmid expressing BA.4 and BA.5 spike protein

    Depositor

    David Nemazee

    Article

    Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

    Insert

    43740568 (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HY69-70del, L452R, F486V, Q493R, on the basis of …

    Promoter

    CMV

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1hSIRT3_H248Y_HA

    Plasmid

    #24492

    Depositor

    Eric Verdin

    Article

    Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

    Insert

    Human Sirtuin 3 catalytically inactive mutant (SIRT3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    H248Y

    Promoter

    Available since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mOtop1_pcDNA3

    Plasmid

    #114677

    Purpose

    This construct was used to express mouse Otop1 in HEK-293 cells

    Depositor

    Emily Liman

    Article

    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

    Insert

    Otopetrin 1 (Otop1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SNP (rs49325632): Glycine 396 to Alanine

    Promoter

    CMV

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3_FLAG_MTA3

    Plasmid

    #31696

    Depositor

    Paul Wade

    Article

    Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

    Insert

    Metastasis associated protein 3 (MTA3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

24. pcDNA3.3_NDG

    Plasmid

    #26820

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Sep 29. ():.

    Insert

    5'UTR-NDGFP-3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    nuclear destabilized eGFP

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

25. pcDNA3-NP

    Plasmid

    #74737

    Purpose

    mammalian expression of the Nucleoprotein of the A/New Caledonia/20/99 (H1N1) Influenza virus

    Depositor

    Elisa Vicenzi

    Article

    Di Pietro et al J Virol. 2013 Apr;87(8):4523-33. doi: 10

    Insert

    Nucleoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1_ionRE

    Plasmid

    #172932

    Purpose

    Ionic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.

    Depositor

    Arnold Boersma

    Article

    Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

    Insert

    IonicStrengthSensorRE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.2/EV

    Plasmid

    #120833

    Purpose

    Control vector, 20nt random sequence between gateway recombination sites

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3_QLuc

    Plasmid

    #183260

    Purpose

    Express QLuc in mammalian cells

    Depositor

    Huiwang Ai

    Article

    Xiong et al J Am Chem Soc. 2022 Aug 1. doi: 10.1021/

    Insert

    QLuc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1hSIRT4_HA

    Plasmid

    #24482

    Depositor

    Eric Verdin

    Article

    Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

    Insert

    Sirtuin 4 (SIRT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.3_SARS2_Mu

    Plasmid

    #177578

    Purpose

    expressing SARS-CoV-2 B.1.621 (Mu) spike protein for pseudovirus production

    Depositor

    David Nemazee

    Article

    Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

    Insert

    Spike (Mu variant) (S SARS-CoV-2)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    T95I, Y144T, Y145S, R346K, E484K, N501Y, D614G, …

    Promoter

    CMV

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3.3_SARS2_Lambda

    Plasmid

    #177579

    Purpose

    expressing SARS-CoV-2 C.37 (lambda) spike protein for pseudovirus production

    Depositor

    David Nemazee

    Article

    Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

    Insert

    Spike (Lambda variant) (S SARS-CoV-2)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    75-76VI, 246-253N, L452Q, F490S, D614G, T859N, la…

    Promoter

    CMV

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCDNA3_Gap43_Csx30_Cre

    Plasmid

    #197604

    Purpose

    Cre reporter for mammalian cells with Gap43 membrane anchor

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2022 Nov 25;378(6622):874-881.

    Insert

    Gap43_Csx30_Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCDNA3_Chrm3_Csx30_Cre

    Plasmid

    #197603

    Purpose

    Cre reporter for mammalian cells with Chrm3 membrane anchor

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2022 Nov 25;378(6622):874-881.

    Insert

    Chrm3_Csx30_Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCDNA3.0_mitoNanobody

    Plasmid

    #136622

    Purpose

    Plasmid encoding outer mitochondrial localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    NTOM20-SNAP-Tag-EnhancerNb
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3_CDC50A

    Plasmid

    #203694

    Purpose

    expresses CDC50A

    Depositor

    Peter Vangheluwe

    Article

    Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

    Insert

    TMEM30A (TMEM30A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcdna3mciap1

    Plasmid

    #11474

    Depositor

    Jon Ashwell

    Article

    Conze et al Mol Cell Biol. 2005 Apr . 25(8):3348-56.

    Insert

    mciap1 (Birc2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2006

    Availability

    Academic Institutions and Nonprofits only

37. pcDNA3_ERBB2-V5

    Plasmid

    #214612

    Purpose

    ERBB2 receptor for ERBB2/ERBB3 heterodimer split TEV recuitment assay

    Depositor

    Michael Wehr

    Article

    Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA_DiCre2_60.F2

    Plasmid

    #60210

    Purpose

    Expresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(60-343)

    Depositor

    Jean-Paul Herman

    Insert

    Cre recombinase (CD207 Bacteriophage P1, Human, Synthetic)
    

    Use

    Tags

    FKBP12(F36V) and Nuclear localisation signal

    Expression

    Mammalian

    Mutation

    FKBP12 has the F36V mutation; amino acids 60-343 …

    Promoter

    CMV

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.1_hMed25

    Plasmid

    #206958

    Purpose

    The ORF of human Med25 cDNA amplified from the RT human liver RNA was cloned into pGEMTE vector and then subclone at XhoI/XbaI sites of the pcDNA3.2(+), the mammalian expression vector.

    Depositor

    Reza Zolfaghari, Catharine Ross

    Insert

    Homo sapiens mediator complex subunit 25 (MED25), transcript variant 1, mRNA (MED25 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. mOtop2_pcDNA3

    Plasmid

    #114678

    Purpose

    This construct was used to express mouse Otop2 in HEK-293 cells

    Depositor

    Emily Liman

    Article

    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

    Insert

    Otopetrin 2 (Otop2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge