We narrowed to 391 results for: SK2

1. CMVTO-KRAS

   Plasmid

   #179653

   Purpose

   KRAS protein

   Depositor

   Xiaojing Gao

   Article

   Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

   Insert

   KRAS (KRAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGL3-KRAS

   Plasmid

   #13919

   Depositor

   Frank Slack

   Article

   Johnson et al Cell. 2005 Mar 11. 120(5):635-47.

   Insert

   KRAS 3'UTR (KRAS Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUG2K

   Plasmid

   #35493

   Depositor

   Ramesh Shivdasani

   Article

   Horst et al Cancer Res. 2012 Feb 8.

   Insert

   KRAS(G12V) (KRAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   Ubiquitin C

   Available since

   May 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. UBQLN2_450-624W

   Plasmid

   #176852

   Purpose

   Expresses human UBQLN2 construct 450-624 with added C-terminal W in bacterial cells

   Depositor

   Carlos Castañeda

   Article

   Dao et al Mol Cell. 2018 Mar 15;69(6):965-978.e6.

   Insert

   UBQLN2_450C_W (UBQLN2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   deletion of residues 1-449 and addition of C-term…

   Promoter

   T7

   Available since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Myc-CK2beta 6KR (pAB1016)

   Plasmid

   #37361

   Depositor

   David Litchfield

   Article

   French et al J Biol Chem. 2007 Oct 5;282(40):29667-77

   Insert

   CK2beta 6KR (CSNK2B Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   K33R, K139R, K177R, K191R, K208R, K212R

   Promoter

   CMV-F

   Available since

   July 17, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pINDUCER21-puro_TAOK1

   Plasmid

   #209777

   Purpose

   doxycycline-inducible expression of TAOK1

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   TAOK1 (TAOK1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. bactin:mKate2-ras

   Plasmid

   #105963

   Purpose

   transgenesis, ubiquitous expression

   Depositor

   Caren Norden

   Article

   Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

   Insert

   Ras (KRAS Human)
   

   Use

   Tags

   mKate2

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTSK405

   Plasmid

   #190817

   Purpose

   For C-terminus 3xGFP tagging of a gene of interest using URA3 selection marker in S. cerevisiae

   Depositor

   Gunjan Mehta, Tatiana Karpova

   Article

   Ball et al Nucleic Acids Res. 2016 Dec 1;44(21):e16

   Insert

   URA3 (URA3 Budding Yeast)
   

   Use

   Bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Calb1-T2A-dgCre targeting vector

   Plasmid

   #114438

   Purpose

   Target dgCre into the endogenous mouse Calb1 locus at the stop codon

   Depositor

   Hongkui Zeng

   Article

   Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

   Insert

   dgCre
   

   Use

   Mouse Targeting

   Tags

   DHFR domain and EGFP

   Expression

   Mutation

   Promoter

   none; utilizes endogenous Calb1 promoter for expr…

   Available since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-PGK-KRAS4B(G12V)

    Plasmid

    #35633

    Depositor

    Daniel Haber

    Article

    Singh et al Cell. 2012 Feb 17;148(4):639-50.

    Insert

    K-Ras4B(G12V) (KRAS Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Glycine 12 to Valine

    Promoter

    PGK

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAB46 (CK2beta)

    Plasmid

    #27085

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2beta (CSNK2B Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-PGK-ER-KRAS(G12V)

    Plasmid

    #35635

    Depositor

    Daniel Haber

    Article

    Singh et al Cell. 2012 Feb 17;148(4):639-50.

    Insert

    K-Ras4B(G12V) (KRAS Human)
    

    Use

    Lentiviral

    Tags

    ER

    Expression

    Mammalian

    Mutation

    Glycine 12 to Valine

    Promoter

    PGK

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pZW12 (CK2beta)

    Plasmid

    #27088

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2beta (CSNK2B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-PGK-KRAS4A(G12V)

    Plasmid

    #35634

    Depositor

    Daniel Haber

    Article

    Singh et al Cell. 2012 Feb 17;148(4):639-50.

    Insert

    K-Ras4A(G12V) (KRAS Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Glycine 12 to Valine

    Promoter

    PGK

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pCGN K-Ras 12V, 188L

    Plasmid

    #14722

    Depositor

    Adrienne Cox

    Article

    Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

    Insert

    K-ras (KRAS Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    12V, 188L

    Promoter

    Available since

    Aug. 29, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pAB07 (CK2beta)

    Plasmid

    #27091

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2beta (CSNK2B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2012

    Availability

    Academic Institutions and Nonprofits only

17. RPS6KA4 gRNA (BRDN0001487122)

    Plasmid

    #77919

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KA4

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KA4 (RPS6KA4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. RPS6KA4 gRNA (BRDN0001146216)

    Plasmid

    #77920

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KA4

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KA4 (RPS6KA4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RPS6KA4 gRNA (BRDN0001149050)

    Plasmid

    #77921

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KA4

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KA4 (RPS6KA4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SRPK2 gRNA (BRDN0001145577)

    Plasmid

    #75599

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SRPK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SRPK2 (SRPK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. SRPK2 gRNA (BRDN0001145583)

    Plasmid

    #75600

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SRPK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SRPK2 (SRPK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. SRPK2 gRNA (BRDN0001146940)

    Plasmid

    #75601

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SRPK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SRPK2 (SRPK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEGFPc3 K-RAS 4B

    Plasmid

    #236452

    Purpose

    transient overexpression of K-RAS 4B in mammalian cells

    Depositor

    Michele Pagano

    Article

    Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

    Insert

    K-RAS 4B (KRAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1-myc-BirA*-KRASG12V

    Plasmid

    #122208

    Purpose

    Expresses myc-BirA* fused to KRASG12V in mammalian cells

    Depositor

    Christopher Counter

    Article

    Adhikari et al Nat Commun. 2018 Sep 7;9(1):3646. doi: 1

    Insert

    KRASG12V (KRAS Human)
    

    Use

    Tags

    myc-BirA*

    Expression

    Mammalian

    Mutation

    changed G12 to V

    Promoter

    CMV

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. 155_KRAS in pmiRGlo

    Plasmid

    #78132

    Purpose

    luciferase reporter for miRNA activity

    Depositor

    Heidi Schwarzenbach

    Article

    Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

    Insert

    KRAS (KRAS Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    3'UTR containing miRNA binding sites

    Promoter

    PGK

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. RPS6KA2 gRNA (BRDN0001148851)

    Plasmid

    #75734

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KA2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KA2 (RPS6KA2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. RPS6KA2 gRNA (BRDN0001147334)

    Plasmid

    #75732

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KA2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KA2 (RPS6KA2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHR-FKBP:mCherry-KRas4Bc30

    Plasmid

    #72900

    Purpose

    Membrane-targeted mCherry-tagged recruiter construct

    Depositor

    Ivan Yudushkin

    Article

    Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

    Insert

    GTPase K-Ras (KRAS Human)
    

    Use

    Lentiviral

    Tags

    FKBP and mCherry

    Expression

    Mammalian

    Mutation

    amino acids 158..188 (fragment 1..157 deleted)

    Promoter

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. KRAS-P34R

    Plasmid

    #158527

    Purpose

    Expresses human KRAS P34R in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

    Depositor

    Kenneth Westover

    Article

    Bera et al Birth Defects Res. 2020 Mar 18. doi: 10.

    Insert

    KRAS (KRAS Human)
    

    Use

    Tags

    6xHis-tag and TEV protease cleavage sequence

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. RPS6KA2 gRNA (BRDN0001149230)

    Plasmid

    #75735

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KA2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KA2 (RPS6KA2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. TAOK1 gRNA (BRDN0001148505)

    Plasmid

    #76806

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TAOK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TAOK1 (TAOK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEGFPc1 K-RAS 4A

    Plasmid

    #236451

    Purpose

    transient overexpression of K-RAS 4A in mammalian cells

    Depositor

    Michele Pagano

    Article

    Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

    Insert

    K-RAS 4A (KRAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. HA-MAP4K6

    Plasmid

    #229401

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    MAP4K6 (MINK1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMAL-MBP-KRas-Q61L

    Plasmid

    #214300

    Purpose

    Express constitutively active KRas fused with MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Constitutively active KRas (KRAS Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    KRas-Q61L

    Promoter

    Tac

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMAL-MBP-KRas-S17N

    Plasmid

    #214301

    Purpose

    Express dominant negative KRas fused with MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Dominant negative KRas (KRAS Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    KRas-S17N

    Promoter

    Tac

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. TAOK1 gRNA (BRDN0001145897)

    Plasmid

    #76807

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TAOK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TAOK1 (TAOK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. TAOK1 gRNA (BRDN0001147719)

    Plasmid

    #76808

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TAOK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TAOK1 (TAOK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. RPS6KA2 gRNA (BRDN0001148104)

    Plasmid

    #75733

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KA2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KA2 (RPS6KA2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBabe K-Ras 12V

    Plasmid

    #12544

    Depositor

    Channing Der

    Article

    Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

    Insert

    K-Ras 12V (KRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    12V constituitively activated

    Promoter

    Available since

    Sept. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLEX-HA-birA*-K-Ras(G12D)-IRES-Puro

    Plasmid

    #120562

    Purpose

    Expresses HA-birA*-K-Ras G12D fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    KRAS4B (KRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    G12D

    Promoter

    CMV

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. TRE-UBC-hCD59-T2A-GFP

    Plasmid

    #205460

    Purpose

    lentiviral plasmid for expression of human CD59

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    hCD59 (CD59 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. R979-X58-008: Hs.KRAS4b(1-188)

    Plasmid

    #159691

    Purpose

    Baculovirus protein expression of KRAS4b (1-188)

    Depositor

    Dominic Esposito

    Article

    Dharmaiah et al Sci Rep. 2019 Jul 19;9(1):10512. doi: 10

    Insert

    Hs.KRAS4b(1-188) (KRAS Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLEX-HA-birA*-K-Ras(wildtype)-IRES-Puro

    Plasmid

    #120561

    Purpose

    Expresses HA-birA*-K-Ras wildtype fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    KRAS4B (KRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. PC4_3spc34h

    Plasmid

    #70700

    Purpose

    Expresses S. cerevisiae DASH/Dam1 complex with C-terminal his-tag on spc34

    Depositor

    Stephen Harrison

    Article

    Miranda et al Nat Struct Mol Biol. 2005 Feb;12(2):138-

    Insert

    DASH / Dam1 complex (DAM1, ASK1, DUO1, SPC34, SPC19, DAD1, DAD2, DAD4, HSK3, DAD3 Budding Yeast)
    

    Use

    Tags

    his-tag on C-terminus of spc34

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. PC4_3dad1h

    Plasmid

    #70699

    Purpose

    Expresses S. cerevisiae DASH/Dam1 complex with C-terminal his-tag on dad1

    Depositor

    Stephen Harrison

    Article

    Miranda et al Nat Struct Mol Biol. 2005 Feb;12(2):138-

    Insert

    DASH / Dam1 complex (DAM1, ASK1, DUO1, SPC34, SPC19, DAD1, DAD2, DAD4, HSK3, DAD3 Budding Yeast)
    

    Use

    Tags

    his-tag on C-terminus of dad1

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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46. W118-1_hRSK3(RPS6KA2)

    Plasmid

    #180384

    Purpose

    lentiviral transduction of human RSK3 (RPS6KA2) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

    Insert

    RPS6KA2 (RPS6KA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. CD59-bio-His

    Plasmid

    #53154

    Purpose

    Expresses full-length CD59 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    CD59 (CD59 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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48. KRAS-V14I

    Plasmid

    #154427

    Purpose

    V14I Kras with Flag tag inserted into N-terminal domain between 6Xhis tag and TEV cleavage site.

    Depositor

    Kenneth Westover

    Article

    Bera et al J Biol Chem. 2019 Sep 20;294(38):13964-1

    Insert

    KRAS (KRAS Human)
    

    Use

    Tags

    6xHis-tag and TEV protease cleavage sequence

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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49. Str-KDEL_SBP-EGFP-CD59

    Plasmid

    #222307

    Purpose

    Synchronize the trafficking of CD59 from the ER.

    Depositor

    Franck Perez

    Article

    Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

    Insert

    Streptavidin-KDEL and CD59 fused to SBP-EGFP (CD59 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    signal peptide removed

    Promoter

    CMV

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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50. pMAL-MBP-FRB-KRas/N12

    Plasmid

    #214302

    Purpose

    Express split-KRas fragment fused with FRB and MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Split-KRas fragment fused with FRB (KRAS Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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