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Showing: 201 - 250 of 18157 results

  5. pET28-AviTag-DogCatcher-MBP

    Plasmid
    #171928
    Purpose
    Expresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    AviTag-DogCatcher-MBP
    Tags
    His6 and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    DogCatcher added to N-terminus of MBP via a GSGES…
    Promoter
    T7
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171928-plasmid-map-sequence-id-338570

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  11. pET28-AviTag-DogTag-MBP

    Plasmid
    #171773
    Purpose
    Expresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    AviTag-DogTag-MBP
    Tags
    His6 and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Peptide tag added to N-terminus of MBP via a GSGE…
    Promoter
    T7
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171773-plasmid-map-sequence-id-338517

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  12. pET28a-HaloTag7SS-DogTag

    Plasmid
    #171775
    Purpose
    Expresses in bacterial cytoplasm HaloTag7 with DogTag inserted between D139 and E140, as well as C61S and C261S in HaloTag7 to block disulfide bond formation
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    HaloTag7SS-DogTag
    Tags
    His6
    Expression
    Bacterial
    Mutation
    DogTag inserted in HaloTag7 between residues D139…
    Promoter
    T7
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171775-plasmid-map-sequence-id-338524

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  13. pET28-MBP-super TEV protease

    Plasmid
    #171782
    Purpose
    Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    MBP-sTEV
    Tags
    Arg6 tag and Internal His6 tag
    Expression
    Bacterial
    Mutation
    No internal TEV cleavage site between the MBP and…
    Promoter
    T7
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171782-plasmid-map-sequence-id-443047

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  16. pNC2

    Plasmid
    #72438
    Purpose
    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1); NCAIR synthetase (EcPurK)
    Depositor
    T. J. Kappock, JoAnne Stubbe
    Article
    Meyer et al Biochemistry. 1999 Mar 9;38(10):3012-8.
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Expression
    Bacterial
    Mutation
    Pro64 to Arg; Asp65 to His; Gln206 to Arg
    Promoter
    T7 and n/a
    Available Since
    May 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72438-plasmid-map-sequence-id-140433

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  18. pAG Lenti CMV Empty Puro

    Plasmid
    #236083
    Purpose
    Empty lentiviral backbone
    Depositor
    Melina Fan
    Type
    Empty backbone
    Use
    Lentiviral
    Promoter
    CMV
    Available Since
    April 25, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236083-plasmid-map-sequence-id-467823

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  19. pAG Lenti CMV C-HA Puro

    Plasmid
    #236080
    Purpose
    Empty lentiviral backbone with C-terminal HA tag
    Depositor
    Melina Fan
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    HA
    Promoter
    CMV
    Available Since
    April 25, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236080-plasmid-map-sequence-id-467818

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  20. pAG Lenti CMV N-HA Puro

    Plasmid
    #236079
    Purpose
    Empty lentiviral backbone with N-terminal HA tag
    Depositor
    Melina Fan
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    HA
    Promoter
    CMV
    Available Since
    April 25, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236079-plasmid-map-sequence-id-467817

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  21. pAG Lenti CMV HA-EGFP Puro

    Plasmid
    #236081
    Purpose
    Lentiviral backbone expressing N-terminally HA tagged EGFP, control for empty backbone 236079
    Depositor
    Melina Fan
    Insert
    EGFP
    Use
    Lentiviral
    Tags
    HA
    Promoter
    CMV
    Available Since
    April 25, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236081-plasmid-map-sequence-id-467821

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  22. pAG Lenti CMV EGFP Puro

    Plasmid
    #236084
    Purpose
    Lentiviral backbone expressing EGFP, control for empty backbone 236083
    Depositor
    Melina Fan
    Insert
    EGFP
    Use
    Lentiviral
    Promoter
    CMV
    Available Since
    April 25, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236084-plasmid-map-sequence-id-467824

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  23. pAG Lenti CMV EGFP-HA Puro

    Plasmid
    #236082
    Purpose
    Lentiviral backbone expressing C-terminally HA tagged EGFP, control for empty backbone 236080
    Depositor
    Melina Fan
    Insert
    EGFP
    Use
    Lentiviral
    Tags
    HA
    Promoter
    CMV
    Available Since
    April 25, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236082-plasmid-map-sequence-id-467819

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  24. MBP-Benzonase

    Plasmid
    #218192
    Purpose
    Bacterial expression of a periplasmically expressed MBP-tagged Serratia Marcescens NucA (Benzonase); also known as c001
    Depositor
    Cheryl Arrowsmith
    Article
    Rieubon et al protocols.io 2025
    Insert
    nucA
    Tags
    MBP
    Expression
    Bacterial
    Available Since
    April 24, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    218192-plasmid-map-sequence-id-431003

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  25. pET-29b(+)_NSP4ss_SerM_NucA-His

    Plasmid
    #218190
    Purpose
    Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)
    Depositor
    Florence Pojer
    Article
    Rieubon et al protocols.io 2025
    Insert
    nucA
    Tags
    His and NSP4 signal secretion peptide
    Expression
    Bacterial
    Mutation
    codon optimized ecoli residues - 22-266
    Promoter
    T7
    Available Since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218190-plasmid-map-sequence-id-467313

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  26. pET-29b(+)_NSP4ss_SerM_NucA-myc

    Plasmid
    #218191
    Purpose
    Bacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)
    Depositor
    Florence Pojer
    Article
    Rieubon et al protocols.io 2025
    Insert
    nucA
    Tags
    NSP4 signal secretion peptide and myc
    Expression
    Bacterial
    Mutation
    codon optimized ecoli residues - 22-266
    Promoter
    T7
    Available Since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218191-plasmid-map-sequence-id-433865

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  36. pET-H-NS-moxCerulean3-15b

    Plasmid
    #250437
    Purpose
    Expresses H-NS-moxCerulean3 in bacterial cells (pET15b backbone)
    Depositor
    Kyubong Jo
    Insert
    H-NS-moxCerulean3
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    Feb. 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    250437-plasmid-map-sequence-id-499767

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  37. tdTomato-C1

    Plasmid
    #54653
    Purpose
    Localization: C1 Cloning Vector, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    tdTomato
    Expression
    Mammalian
    Available Since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    54653-plasmid-map-sequence-id-450928

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  38. DsRed2-C1

    Plasmid
    #54611
    Purpose
    Localization: C1 Cloning Vector, Excitation: 558, Emission: 583
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    DsRed2
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54611-plasmid-map-sequence-id-257389

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  39. EBFP2-C1

    Plasmid
    #54665
    Purpose
    Localization: C1 Cloning Vector, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    EBFP2
    Expression
    Mammalian
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54665-plasmid-map-sequence-id-151383

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  40. ZsGreen1-N1

    Plasmid
    #54702
    Purpose
    Localization: N1 Cloning Vector, Excitation: 493, Emission: 505
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    ZsGreen1
    Expression
    Mammalian
    Available Since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    54702-plasmid-map-sequence-id-266653

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  41. mMaroon-N1

    Plasmid
    #54554
    Purpose
    Localization: N1 Cloning Vector, Excitation: 610, Emission: 652
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mMaroon
    Expression
    Mammalian
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54554-plasmid-map-sequence-id-475388

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  42. DsRed2-N1

    Plasmid
    #54493
    Purpose
    Localization: N1 Cloning Vector, Excitation: 558, Emission: 583
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    DsRed2
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54493-plasmid-map-sequence-id-352456

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  43. EBFP-C1

    Plasmid
    #54738
    Purpose
    Localization: C1 Cloning Vector, Excitation: 387, Emission: 450
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    EBFP
    Expression
    Mammalian
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54738-plasmid-map-sequence-id-256475

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  44. mRuby-N1

    Plasmid
    #54581
    Purpose
    Localization: N1 Cloning Vector, Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mRuby
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54581-plasmid-map-sequence-id-393318

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  45. Dronpa3-N1

    Plasmid
    #54682
    Purpose
    Localization: N1 Cloning Vector, Excitation: 503, Emission: 518
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    Dronpa3
    Expression
    Mammalian
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54682-plasmid-map-sequence-id-139033

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  46. mNeptune2-N1

    Plasmid
    #54837
    Purpose
    Localization: N1 Cloning Vector, Excitation: 600, Emission: 650
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mNeptune2
    Expression
    Mammalian
    Available Since
    June 11, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54837-plasmid-map-sequence-id-162708

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  47. AcGFP1-C1

    Plasmid
    #54607
    Purpose
    Localization: C1 Cloning Vector, Excitation: 475, Emission: 505
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    AcGFP1
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    54607-plasmid-map-sequence-id-162738

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  48. AcGFP1-N1

    Plasmid
    #54705
    Purpose
    Localization: N1 Cloning Vector, Excitation: 475, Emission: 505
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    AcGFP1
    Expression
    Mammalian
    Available Since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    54705-plasmid-map-sequence-id-162699

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  49. mMaroon-C1

    Plasmid
    #54835
    Purpose
    Localization: C1 Cloning Vector, Excitation: 610, Emission: 652
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mMaroon
    Expression
    Mammalian
    Available Since
    June 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54835-plasmid-map-sequence-id-256278

    Enlarge

  50. mNeptune2-C1

    Plasmid
    #54836
    Purpose
    Localization: C1 Cloning Vector, Excitation: 600, Emission: 650
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mNeptune2
    Expression
    Mammalian
    Available Since
    June 11, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54836-plasmid-map-sequence-id-132753

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Showing: 201 - 250 of 18157 results