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Showing: 201 - 250 of 11662 results

  1. e-ciCas9_pcDNA5

    Plasmid
    #100553
    Purpose
    Expresses enhanced specificity ciCas9 (e-ciCas9) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.
    Depositor
    Dustin Maly
    Article
    Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n
    Insert
    e-ciCas9
    Use
    CRISPR; Frt, flp-in
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    100553-plasmid-map-sequence-id-193863

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  3. pcDNA3_erGAP2

    Plasmid
    #78120
    Purpose
    Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum
    Depositor
    Teresa Alonso, Javier García-Sancho
    Insert
    erGAP2
    Use
    Tags
    KDEL and calreticulin signal peptide
    Expression
    Mammalian
    Mutation
    D24N/D119A in aequorin moiety
    Promoter
    CMV
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    78120-plasmid-map-sequence-id-153381

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  7. pcDNA_DiCre2_59.F2

    Plasmid
    #60209
    Purpose
    Expresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(19-59)
    Depositor
    Jean-Paul Herman
    Insert
    Cre recombinase (CD207 Human, Bacteriophage P1, Synthetic)
    Use
    Tags
    FKBP12(F36V) and Nuclear localisation signal
    Expression
    Mammalian
    Mutation
    FKBP12 has the F36V mutation; amino acids 19-59 o…
    Promoter
    CMV
    Available since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60209-plasmid-map-sequence-id-96295

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  12. IRE1bpcDNA3

    Plasmid
    #21879
    Depositor
    David Ron
    Insert
    IRE1b (Ern2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    21879-plasmid-map-sequence-id-10060

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  15. pCDNA3_AKAR5Δ

    Plasmid
    #119912
    Purpose
    Mammalian expression of the AKAR5Δ sensor with N-terminal His tag for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)
    Depositor
    Haining Zhong
    Article
    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do
    Insert
    AKAR5Δ
    Use
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    119912-plasmid-map-sequence-id-235127

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  21. ERroGFP_iE_C204Q_pCDNA3

    Plasmid
    #48629
    Purpose
    Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 204 to Glutamine mutation (roGFP C147, E147b, C204Q)
    Depositor
    David Ron
    Article
    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.
    Insert
    ERroGFP
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    insertion E147B, C204Q
    Promoter
    Available since
    Oct. 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    48629-plasmid-map-sequence-id-70377

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  23. ERroGFP_C147S_pCDNA3

    Plasmid
    #48630
    Purpose
    Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFP with Cysteine 147 to Serine mutation (roGFP C147S, C204)
    Depositor
    David Ron
    Article
    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.
    Insert
    ERroGFP
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    C147S
    Promoter
    Available since
    Oct. 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    48630-plasmid-map-sequence-id-70378

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  26. ERrefGFP1_pCDNA3

    Plasmid
    #48635
    Purpose
    Mam vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with mut Cys147 to Ser, del insertion147b and mut Cys204 to Glut.Referred to as ref GFP
    Depositor
    David Ron
    Article
    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.
    Insert
    ERrefGFP1
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    C147S,C204Q
    Promoter
    Available since
    Oct. 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    48635-plasmid-map-sequence-id-70401

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  42. pcDNA3.1_hCAR.Variant3

    Plasmid
    #209133
    Purpose
    The ORF of human constitutive androstane receptor (CAR), variant 3 cDNA amplified from the RT human liver RNA was cloned at the NheI/HindIII sites of the pcDNA3.1(+), the mammalian expression vector.
    Depositor
    Reza Zolfaghari, Catharine Ross
    Insert
    constitutive androstane receptor (CAR), Variant 3 (NR1I3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    209133-plasmid-map-sequence-id-422366

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  47. pcDNA3.1_rSERT_C109A_Y107C

    Plasmid
    #190174
    Purpose
    For expression of a SERT mutant in mammalian cells and measuring accessibility in the SERT extracellular pathway
    Depositor
    Gary Rudnick
    Article
    Jacobs et al J Biol Chem. 2007 Oct 5;282(40):29441-7.
    Insert
    SLC6A4 Serotonin Transporter (Slc6a4 Rat)
    Use
    Tags
    FLAG, His6, and c-myc
    Expression
    Mammalian
    Mutation
    changed Cys109 to alanine, changed tyr107 to cyst…
    Promoter
    CMV
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190174-plasmid-map-sequence-id-377202

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Showing: 201 - 250 of 11662 results