We narrowed to 29,325 results for: REP

1. gRNA_tdtomato_reporter_1_ST1_2x_binding

   Plasmid

   #79368

   Purpose

   Assays activity of transcriptional activators fused to ST1 Cas9

   Depositor

   George Church

   Article

   Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

   Insert

   tdtomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pREP3xN_ccdB

   Plasmid

   #79021

   Purpose

   Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, LEU2 selection

   Depositor

   Pernilla Bjerling, Göran Akusjärvi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pREP4xV5_ccdB

   Plasmid

   #79016

   Purpose

   Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ selection

   Depositor

   Pernilla Bjerling, Göran Akusjärvi

   Type

   Empty backbone

   Use

   Tags

   double V5 epitope tag

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pREP3xV5_ccdB

   Plasmid

   #79015

   Purpose

   Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, LEU2 selection

   Depositor

   Pernilla Bjerling, Göran Akusjärvi

   Type

   Empty backbone

   Use

   Tags

   double V5 epitope tag

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pREP82xN_ccdB

   Plasmid

   #79026

   Purpose

   Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selection

   Depositor

   Pernilla Bjerling, Göran Akusjärvi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pREP81xN_ccdB

   Plasmid

   #79025

   Purpose

   Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selection

   Depositor

   Pernilla Bjerling, Göran Akusjärvi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CaMK2rep

   Plasmid

   #239622

   Purpose

   CaMKII activity reporter. The plasmid contains sGFP2 fluorescent protein, 3xmyc tags and double phospho-site 3 from rat Synapsin-1a.

   Depositor

   F Javier Díez Guerra

   Insert

   SpotNES-sGFP2-SynP3-3xmyc (Syn1 Rat, Synthetic)
   

   Use

   Tags

   Spot, myc, and sGFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. nCaMK2rep

   Plasmid

   #239624

   Purpose

   CaMKII activity reporter. The lentiviral plasmid contains a nanobody sequence (PSD95.FingR), 3xmyc tags and double phospho-site 3 from rat Synapsin-1a.

   Depositor

   F Javier Díez Guerra

   Insert

   PSD95.FingR-NES-SynP3-3xmyc (Syn1 Rat, Synthetic)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Flag and myc

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3_ST3GAL4_SOX9repall

   Plasmid

   #232399

   Purpose

   Enhancer near ST3GAL4, all buffering elements replaced with SOX9 sites

   Depositor

   Joanna Wysocka

   Insert

   ST3GAL4 (ST3GAL4 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Enhancer near ST3GAL4, all buffering elements rep…

   Promoter

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGL3_FAM153CP_SOX9rep

    Plasmid

    #232400

    Purpose

    Enhancer near FAM153CP, buffering element replaced with SOX9 site

    Depositor

    Joanna Wysocka

    Insert

    FAM153CP (FAM153CP Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Enhancer near FAM153CP, buffering element replace…

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. insulator_seq_reporter_vector

    Plasmid

    #221460

    Purpose

    Vector containing eGFP and mCherry reporter genes, for assembling an insulator-seq reporter library.

    Depositor

    Maria Cristina Gambetta

    Article

    Tonelli et al STAR Protoc. 2024 Oct 24;5(4):103391. do

    Insert

    gypsy insulator-EGFP-neutral spacer DNA-mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSliceRep_B_miR-7

    Plasmid

    #220468

    Purpose

    Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    SUMO_Eu1
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal CMV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSliceRep_B_miR-7.M4

    Plasmid

    #220469

    Purpose

    Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    SUMO_Eu1
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal CMV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSliceRep_A_miR-7

    Plasmid

    #220464

    Purpose

    Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    Superfolder GFP
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal CMV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSliceRep_A_miR-7.M4

    Plasmid

    #220465

    Purpose

    Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    Superfolder GFP
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal CMV

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJP_Rep00

    Plasmid

    #219677

    Purpose

    Light-inducible repressilator (modified from pLPT107). PT3lacO promoter controls the expression of TetR node.

    Depositor

    Yolanda Schaerli

    Article

    Park et al Nat Commun. 2024 Aug 23;15(1):7284. doi:

    Insert

    PT3lacO
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PT3lacO

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pReporter_ZF2_ZF1_mNeonGreen

    Plasmid

    #213834

    Purpose

    Facilitates genomic integration of the reporter for screen 1: ZF-1 followed by ZF-2 upstream of the reporter gene.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pReporter_ZF1_mNeonGreen_ZF2

    Plasmid

    #213835

    Purpose

    Facilitates genomic integration of the reporter for screen 2: ZF-1 upstream of the reporter gene and ZF-2 downstream.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pReporter_ZF1_ZF2_mNeonGreen

    Plasmid

    #213836

    Purpose

    Facilitates genomic integration of the reporter for screen 3: ZF-2 followed by ZF-1 upstream of the reporter gene.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pReporter_ZF2_mNeonGreen_ZF1

    Plasmid

    #213837

    Purpose

    Facilitates genomic integration of the reporter for screen 4: ZF-2 upstream of the reporter gene and ZF-1 downstream.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge