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LbdCas12a VPR

Plasmid

#188513

Purpose

Expresses FLAG tagged LbdCas12a VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12a

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D832A

Promoter

EF1a

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LbdCas12a KRAB

Plasmid

#188503

Purpose

Expresses FLAG tagged LbdCas12a KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12a

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D832A

Promoter

EF1a

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMBP-AaCas12b

Plasmid

#113433

Purpose

Expresses Expresses 10xHis-MBP fused to AaCas12b

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

AaCas12b

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pMV261-Cas12a

Plasmid

#158707

Purpose

expresses codon-optimized FnCpf1

Depositor

Yi-Cheng Sun

Article

Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

FnCpf1

Use

Tags

Expression

Bacterial

Mutation

Promoter

TetO

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pNOC_hfnCas12a-Nlux

Plasmid

#176239

Purpose

pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux under the control of Ribi promoter

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

luciferase

Expression

Mutation

Promoter

Ribosomal subunit

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pET47b-T7-AsCas12f-WT

Plasmid

#204640

Purpose

Expression of wild-type AsCas12f in E. coli

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

AsCas12f

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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CL7-LbCas12a

Plasmid

#164658

Purpose

Bacterial expression of LbCas12a using CL7/Im7

Depositor

Piyush Jain

Article

Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

Insert

LbCas12a

Use

Tags

Thioredoxin, CL7, SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-AsCas12f-WT

Plasmid

#204635

Purpose

Expression of wild-type AsCas12f in mammalian cells

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

AsCas12f

Use

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pNOC_hlbCas12a-Nlux

Plasmid

#176240

Purpose

pNOC episomal plasmid harboring the humanized lbCas12a gene sequence tagged with Nlux under the control of Ribi promoter

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized lbCas12a

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

luciferase

Expression

Mutation

Promoter

Ribosomal subunit

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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CL7-LbCas12aD156R

Plasmid

#164659

Purpose

Bacterial expression of mutated LbCas12aD156R using CL7/Im7

Depositor

Piyush Jain

Article

Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

Insert

LbCas12a-D156R

Use

Tags

Thioredoxin, CL7, SUMO

Expression

Bacterial

Mutation

changed Aspartic Acid 156 to Arginine

Promoter

T7

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ281 (MbCas12a)

Plasmid

#138113

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized MbCas12a without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

MbCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ-NF-L3-NF-L1-dMb2Cas12a

Plasmid

#177686

Purpose

Gateway entry clone (attL1 & attR5) for a single chain FokI-dMb2Cas12a genome editing

Depositor

Yiping Qi

Article

Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

Insert

2xFokI-dMb2Cas12a

Use

CRISPR; Gateway compatible 2xfoki-dmb2cas12a entr…

Tags

NLS

Expression

Plant

Mutation

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCJH059_proD_Cas12c_DualRNA-GFP_CAM

Plasmid

#183076

Purpose

Plasmid expressing Cas12c tracrRNA and a GFP-targeting pre-crRNA separately.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c tracrRNA and pre-crRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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dCas12j2 KRAB

Plasmid

#188508

Purpose

Expresses FLAG tagged dCas12j2 KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12j2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D394A

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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Cas12f-GE ver4.0

Plasmid

#176543

Purpose

Expresses Cas12f-GE in mammalian cells

Depositor

Yong-Sam Kim

Article

Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

Inserts

Cas12f-GE ver4.0
Cas12f-GE ver4.0

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and chicken β-actin

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pCJH019_SC101_pLAC_Cas12c(4)_Kan

Plasmid

#183071

Purpose

Wild-type Cas12c_4 in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c_4

Use

Tags

Expression

Bacterial

Mutation

Wild-type

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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Cas12f-GE ver3.0

Plasmid

#176508

Purpose

Expresses Cas12f-GE in mammalian cells

Depositor

Yong-Sam Kim

Article

Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

Inserts

Cas12f-GE ver3.0
Cas12f-GE ver3.0

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and chicken β-actin

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ-NF-L1-dMb2Cas12a

Plasmid

#177683

Purpose

Gateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editing

Depositor

Yiping Qi

Article

Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

Insert

FokI-dMb2Cas12a

Use

CRISPR; Gateway compatible foki-dmb2cas12a entry …

Tags

NLS

Expression

Plant

Mutation

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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AsCas12a-DR-cr in PBCSK-

Plasmid

#126640

Purpose

Cloning vector AsCas12a DR-crRNA for expression in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

AsCas12a Full Length Direct Repeat crRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ284 (Mb2Cas12a)

Plasmid

#138116

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

Mb2Cas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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