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Showing: 81 - 100 of 128 results

  1. pSC-mCh-P2A-ST-EGFP-CAAX

    Plasmid
    #207636
    Purpose
    A plasmid encoding photocaged SpyCatcher (pSC) fused to mCherry and a SpyTag-EGFP localized to the cell membrane via CAAX tag
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    photocaged SpyCatcher-mCh-P2A-SpyTag-EGFP-CAAX
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Amber stop codon at SpyCatcher’s critical lysine
    Promoter
    CMV
    Available since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207636-plasmid-map-sequence-id-409876

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  2. nCre-ST-NLS-P2A-NLS-pSC-cCre

    Plasmid
    #207638
    Purpose
    A plasmid encoding N-terminal Cre fused to SpyTag (ST) and photocaged SpyCatcher (pSC) fused to C-terminal Cre with a nuclear localization signal (NLS) sequence
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    nCre-SpyTag-NLS-P2A-NLS-photocaged SpyCatcher-cCre
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Amber stop codon at SpyCatcher’s critical lysine
    Promoter
    CMV
    Available since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207638-plasmid-map-sequence-id-409880

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  17. pSC-LPETG

    Plasmid
    #207629
    Purpose
    A plasmid encoding photocaged SpyCatcher (pSC) with a C-terminal Sortase recognition sequence.
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    photocaged SpyCatcher-LPETG
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Amber stop codon at SC's critical lysine
    Promoter
    T7
    Available since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207629-plasmid-map-sequence-id-409853

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  19. EGFP-ST-P2A-pSC-CAAX

    Plasmid
    #207637
    Purpose
    A plasmid encoding EGFP fused to SpyTag (ST) and photocaged SpyCatcher (pSC) localized to the cell membrane via CAAX tag.
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    EGFP-SpyTag-P2A-photocaged SpyCatcher-CAAX
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Amber stop codon at SC's critical lysine
    Promoter
    CMV
    Available since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207637-plasmid-map-sequence-id-409879

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  20. pSC-cUnaG-LPETG

    Plasmid
    #207635
    Purpose
    A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment and LPETG sortase recognition sequence.
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    photocaged SpyCatcher-GGSGGGSG-cUnaG-LPETG
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Amber stop codon at SC's critical lysine
    Promoter
    T7
    Available since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207635-plasmid-map-sequence-id-409865

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Showing: 81 - 100 of 128 results