We narrowed to 4,231 results for: Pet2

1. pET21a Bm-iPGM-bio

   Plasmid

   #162565

   Purpose

   Expresses B. malayi iPGM containing a C-terminal biotinylation site and His tag

   Depositor

   James Inglese

   Article

   Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

   Insert

   B. malayi iPGM bio-6His
   

   Use

   Tags

   6His tag, T7 tag, biotinylation sequence, and thr…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET24b-SUMO-BG2.4

   Plasmid

   #199438

   Purpose

   expresses BAmGreen2.4 in bacterial cells with a SUMO tag

   Depositor

   Andrew Woolley

   Article

   Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

   Insert

   BAMGreen2.4
   

   Use

   Tags

   his tag and sumo

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21a-C2m2-SNAP

   Plasmid

   #208773

   Purpose

   To express C2m2-SNAP in bacteria

   Depositor

   Urte Neniskyte

   Article

   Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

   Insert

   C2m2-SNAP (Mfge8 Mouse)
   

   Use

   Tags

   SNAP-tag

   Expression

   Bacterial

   Mutation

   Mutated 24 and 45 lysine to asparagine in C2 doma…

   Promoter

   T7 promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21b-Caspase-3(V266E)

   Plasmid

   #90089

   Purpose

   Expresses caspase 3 V266E mutant in bacterial cells

   Depositor

   Clay Clark

   Article

   Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

   Insert

   Caspase 3 (CASP3 Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   V266E

   Promoter

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28_mouse-pro-IL1B

   Plasmid

   #183386

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   pro-IL1B (Il1b Mouse)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28b-miniE-DBCO

   Plasmid

   #181985

   Purpose

   Used to label proteins with the MiniR genetically encoded tag

   Depositor

   Kimberly Beatty

   Article

   Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

   Insert

   MiniE-DBCO
   

   Use

   Tags

   DBCO-Tag, His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-His-hAos1

   Plasmid

   #53135

   Purpose

   E.coli expression of full length human Aos1 (SAE1) with Nterminal His-tag

   Depositor

   Frauke Melchior

   Article

   Pichler et al Cell. 2002 Jan 11;108(1):109-20.

   Insert

   Aos1 (SAE1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28C-mCherry-MED1LCD

   Plasmid

   #204404

   Purpose

   E. coli expression of MED1 low-complexity domain (947-1581) with an N-terminal mCherry tag

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   MED1(947-1581) (MED1 Human)
   

   Use

   Tags

   6x-His; mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-eGFP-MinD

   Plasmid

   #133622

   Purpose

   Expression vector for the Escherichia coli MinD with an N-terminal fusion of a His-tag for purification and an EGFP for visualization.

   Depositor

   Petra Schwille

   Article

   Zieske et al FEBS Lett. 2014 Aug 1;588(15):2545-9. do

   Insert

   EGFP-MinD (minD E. coli)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. 6his-WT gusA-pET28a+

    Plasmid

    #61156

    Purpose

    N -terminal His tagged wild-type GUS expression vector

    Depositor

    Andrew Ellington, Ichiro Matsumura

    Article

    Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

    Insert

    gusA
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-LgBiT-SpyCatcher

    Plasmid

    #223634

    Purpose

    Protein purification of SpyCatcher003 (S49C) with LgBiT and 6xHis tags from E. coli

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    SpyCatcher003 S49C
    

    Use

    Tags

    His6, LgBiT

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-HisYFP-Sp100

    Plasmid

    #53141

    Purpose

    E.coli expression of HisYFP tagged human Sp100 fragment

    Depositor

    Frauke Melchior

    Article

    Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

    Insert

    Sp100A fragment (SP100 Human)
    

    Use

    Tags

    His-YFP

    Expression

    Bacterial

    Mutation

    fragment (amino acids 71-480); deletion of L75

    Promoter

    T7

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28_6xHis-PARP1-CATdeltaHD

    Plasmid

    #174811

    Purpose

    Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-CATdeltaHD (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Residues 678-787 replaced by eight-residue linker…

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28C-mCherry-HNRNPA1LCD

    Plasmid

    #204405

    Purpose

    E. coli expression of HNRNPA1 low-complexity domain (186-320) with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    HNRNPA1 (186-320) (HNRNPA1 Human)
    

    Use

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21b-RBD-JA1

    Plasmid

    #167126

    Purpose

    Expresses SARS-CoV-2 RBD in bacterial system

    Depositor

    David Margulies

    Article

    Ahmad et al bioRxiv. 2021 Jan 27. doi: 10.1101/2021.

    Insert

    SARS-CoV-2 Receptor Binding domain (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    residues (333 to 529 aa)

    Promoter

    T7

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28C-mCherry-DDX4LCD

    Plasmid

    #204408

    Purpose

    E. coli expression of DDX4 low-complexity domain (1-236) with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    DDX4(1-236) (DDX4 Human)
    

    Use

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-TEV-TBP6.9F34MF37MF77M

    Plasmid

    #168268

    Purpose

    Expresses human TBP6.9 with the F34M/F37M/F77M triple mutant

    Depositor

    Joseph Wedekind

    Article

    Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

    Insert

    TBP6.9
    

    Use

    Tags

    6xHis tag N-terminus, TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    changed F34M, F37M and F77M

    Promoter

    T7 promotor

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21b-Tipin-His

    Plasmid

    #23122

    Depositor

    Aziz Sancar

    Article

    Kemp et al J Biol Chem. 2010 Mar 15. ():.

    Insert

    Tipin (TIPIN Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pET28a-mH6-Cas12c1

    Plasmid

    #120872

    Purpose

    Expresses E. coli codon optimized Cas12c1 effector in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Cas12c1
    

    Use

    CRISPR

    Tags

    mH6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    lac

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-mH6-Cas12c2

    Plasmid

    #120873

    Purpose

    Expresses E. coli codon optimized Cas12c2 effector in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Cas12c2
    

    Use

    CRISPR

    Tags

    mH6

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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