We narrowed to 6,265 results for: pcDNA 3.1

Showing: 1471 - 1500 of 6265 results

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pcDNA3.1(-) MID51 4xMyc Hisx6

Plasmid

#44598

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

MiD51 (Mief1 Mouse)

Tags

4xMyc and 6xHis

Expression

Mammalian

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-ASAP2s

Plasmid

#101274

Purpose

Genetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

Insert

ASAP2s

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-STIM1-Venus-173-C

Plasmid

#87619

Purpose

Full-length STIM1 fused to C-terminal Venus (YFP) fragment; for BiFC.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

STIM1-Venus173-C (STIM1 Human, Synthetic)

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hAsCpf1(TYCV) (pY210)

Plasmid

#89351

Purpose

Expresses humanized AsCpf1 variant that recognizes TYCV PAMs

Depositor

Feng Zhang

Article

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1, RR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

S542R/K607R

Promoter

CMV

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-PdCO-mScarlet

Plasmid

#198507

Purpose

Expresses optimized PdCO in frame with mScarlet under control of CMV promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2CP-VPg(FCV)

Plasmid

#167314

Purpose

Template pDNA to prepare CaVT mRNA

Depositor

Hirohide Saito

Article

Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

Insert

CaVT

Use

Synthetic Biology

Tags

DYK-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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XE251 pCDNA3.1(zeo)-hDsh3

Plasmid

#16758

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

FLAG-hDsh3 (DVL3 Human)

Tags

flag

Expression

Mammalian

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-DRD2-cpHalo∆-mEGFP

Plasmid

#194679

Purpose

CMV driven expression of D2R GPCR recorder in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

DRD2_cpHalo∆

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Human NCKX6-FlagTag (pcDNA3.1+)

Plasmid

#75217

Purpose

Mammalian expression of SLC8B1

Depositor

Jonathan Lytton

Article

Cai et al J Biol Chem. 2004 Feb 13;279(7):5867-76.

Insert

SLC8B1 (SLC8B1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+(rInsp)-Ins-GLuc

Plasmid

#89928

Purpose

Expresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.

Depositor

Melanie Cobb

Article

Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E

Insert

human insulin-Gaussia-Luciferase (INS Human, Synthetic)

Expression

Mammalian

Mutation

DNA sequence for Gaussia luciferase was humanized…

Promoter

rat Insulin II promoter

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1D V5-His-TOPO-KGA.wt

Plasmid

#110332

Purpose

Mammalian expression of glutaminase isoform KGA (wild type) with V5 and 6xHis tags

Depositor

Sandra Martha Gomes Dias

Article

Cassago et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

Insert

KGA glutaminase (GLS Human)

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ eiF4A2 myc HIS

Plasmid

#71658

Purpose

mammalian expression of eiF4A2

Depositor

Gideon Dreyfuss

Insert

EIF4A2 (EIF4A2 Human)

Tags

HIS and myc

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-2xFLAG-2xSTREP-CDK2

Plasmid

#172616

Purpose

Expresses 2xFLAG-2xSTREP-tagged CDK2 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDK2 (CDK2 Human)

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-JEDI-1P

Plasmid

#202607

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-1P, expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+(Zeo)-FLAG-muGFP

Plasmid

#166726

Purpose

Expresses FLAG-tagged monomeric ultrastable GFP (muGFP) in mammalian cells

Depositor

Daniel Scott

Article

Scott et al Sci Rep. 2018 Jan 12;8(1):667. doi: 10.1

Insert

muGFP

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-FbxO11-ΔFbox-pcDNA3.1-

Plasmid

#52502

Purpose

expresses human FbxO11-ΔFbox in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

FbxO11 (FBXO11 Human)

Tags

FLAG-HA

Expression

Mammalian

Mutation

deletion of Fbox (deleted aa 71-116)

Promoter

CMV

Available Since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cerulean-Epac2-Venus

Plasmid

#138196

Purpose

FRET-based biosensor for monitoring Epac2 activation.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2-Venus (Rapgef4 Mouse, Synthetic)

Tags

Cerulean and Venus

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 S D614

Plasmid

#158074

Purpose

Expresses SARS-CoV-2 spike protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 spike (S )

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8-FLAG

Plasmid

#169948

Purpose

Mammalian expression of CARD8-FLAG

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GFP-FUS-IDR

Plasmid

#183236

Purpose

Expression of eGFP fused to a intrinsically disordered regions of the FUS protein

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

EGFP

Tags

FLAG tag and Fusion to an IDR from the FUS protei…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Rho-EGFP-ETSQVAPA

Plasmid

#45399

Depositor

David Williams

Article

Trivedi et al J Neurosci. 2012 Aug 1;32(31):10587-93.

Insert

Rhodopsin (RHO Bovine)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)/Puro/TagRFP-LL5b

Plasmid

#112828

Purpose

transient/stable overexpression

Depositor

Tomasz Prószyński, Joshua Sanes

Article

Proszynski et al J Cell Sci. 2013 May 15;126(Pt 10):2225-

Insert

LL5b (Phldb2 Mouse)

Tags

TagRFP

Expression

Mammalian

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-2A-eGFP(W58X) pcDNA3.1(-)

Plasmid

#172229

Purpose

Expresses an mCherry-2A-eGFP dual reporter that contains a UAG codon in position 58 (W58X) of eGFP, in mammalian cells

Depositor

Joshua Rosenthal

Article

Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15

Insert

mCherry-2A-eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRM3-cpHalo∆-mEGFP

Plasmid

#194678

Purpose

CMV driven expression of the m3 GPCR recorder in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

CHRM3_cpHalo∆

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1D V5-His-TOPO-HuR.wt

Plasmid

#110417

Purpose

Mammalian expression of HuR (wild type)

Depositor

Sandra Martha Gomes Dias

Insert

HuR (ELAVL1) (ELAVL1 Human)

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) mouse Egr2

Plasmid

#107997

Purpose

Expresses mouse Egr2 in mammalian cells

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr2 (Egr2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-KB1691-Nluc

Plasmid

#204324

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

KB1691-Nluc

Expression

Mammalian

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-TAT-1-101-FLAG

Plasmid

#35981

Depositor

Susana Valente

Article

Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

Insert

transactivating protein (tat )

Tags

Flag

Expression

Mammalian

Mutation

tat from HIV1 101 amino acids

Available Since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 CIM5 (Rpt-1) GFP

Plasmid

#16074

Depositor

Erin Schuman

Article

Bingol et al Nature. 2006 Jun 29. 441(7097):1144-8.

Insert

CIM5 (RPT1 Budding Yeast)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-RIαΔ(D/D+linker)-EGFP

Plasmid

#181843

Purpose

GFP-tagged PKA RIα subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

RIα-(D/D+linker)EGFP (PRKAR1A Human)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of residues 12-112 in PKA RIα.

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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