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Showing: 1961 - 2000 of 64857 results

  3. HDM-SARS2-Spike-delta21

    Plasmid
    #155130
    Purpose
    Encodes SARS-CoV-2 Spike with 21 aa C-terminal deletions for lentiviral pseudotyping
    Depositor
    Jesse Bloom
    Article
    Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051
    Insert
    SARS-CoV-2 Spike-delta21 (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Codon optimized to H. sapiens using IDT codon opt…
    Promoter
    CMV
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    155130-plasmid-map-sequence-id-306280

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  4. TFORF0981

    Plasmid
    #141778
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    KLF6 (KLF6 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141778-plasmid-map-sequence-id-391421

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  6. pPICZc-HsACTG1*-thymosinB-8His

    Plasmid
    #111147
    Purpose
    Expresses human gamma-actin (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag.
    Depositor
    Mohan Balasubramanian
    Article
    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827
    Insert
    actin gamma 1 (ACTG1 Human)
    Use
    Pichia pastoris integration vector
    Tags
    Thymosin beta and a His-tag
    Expression
    Yeast
    Mutation
    codon-optimised for expression in Pichia pastoris
    Promoter
    AOX1
    Available since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    111147-plasmid-map-sequence-id-214068

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  7. pMEXneo-TrkC-TrkB-hybrid

    Plasmid
    #190205
    Purpose
    To express a Human TrkC receptor that binds only BDNF. The second IgG like domain which is the main binding domain comes from the TrkB receptor
    Depositor
    Pantelis Tsoulfas
    Article
    Urfer et al J Biol Chem. 1998 Mar 6;273(10):5829-40.
    Insert
    TrkC (NTRK3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    The second IgG like domain and the extracellular …
    Promoter
    Moloney mouse sarcoma virus right LTR
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190205-plasmid-map-sequence-id-374467

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  8. pABD1031

    Plasmid
    #68004
    Purpose
    Protein co-localization of PIF1 (PIL5) via fluorescent fusion after transient expression in planta
    Depositor
    Bruce Downie
    Article
    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11
    Insert
    PHY-INTERACTING FACTOR 1 (PIF1) (PIL5 )
    Use
    Tags
    eYFP
    Expression
    Plant
    Mutation
    Promoter
    2X Cauliflower Mosaic Virus 35S promoter
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  9. pABD1275

    Plasmid
    #68017
    Purpose
    PIF1 protein overproduction for purification from bacterial cells
    Depositor
    Bruce Downie
    Article
    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11
    Insert
    PHY-INTERACTING FACTOR 1 (PIF1) (PIL5 AT2G20180, Mustard Weed)
    Use
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression (GenScri…
    Promoter
    T7
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  13. pCGT

    Plasmid
    #99030
    Purpose
    pCG empty vector containing T7 tag
    Depositor
    Winship Herr
    Type
    Empty backbone
    Use
    Tags
    T7
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    99030-plasmid-map-sequence-id-192626

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  14. pRS_SN_400

    Plasmid
    #170472
    Purpose
    To amplify transcription template containing LacI operators
    Depositor
    Laura Finzi
    Insert
    T7A1, Os, O-, Lambda t1 in lacZ fragment
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7A1
    Available since
    Sept. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170472-plasmid-map-sequence-id-336808

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  15. pYY_S2_400

    Plasmid
    #170471
    Purpose
    To amplify transcription template containing LacI operators
    Depositor
    Laura Finzi
    Insert
    T7A1, Os, O2, Lambda t1 in lacZ fragment
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7A1
    Available since
    Sept. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170471-plasmid-map-sequence-id-339114

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  16. pYY_N1_400

    Plasmid
    #170473
    Purpose
    To amplify transcription template containing LacI operators
    Depositor
    Laura Finzi
    Insert
    T7A1, O-, O1, Lambda t1 in lacZ fragment
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7A1
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    170473-plasmid-map-sequence-id-336805

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  17. pRS_2S_400

    Plasmid
    #170478
    Purpose
    To amplify transcription template containing LacI operators
    Depositor
    Laura Finzi
    Insert
    T7A1, O2, Os, Lambda t1 in lacZ fragment
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7A1
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    170478-plasmid-map-sequence-id-336813

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  18. pRS_2N_400

    Plasmid
    #170479
    Purpose
    To amplify transcription template containing LacI operators
    Depositor
    Laura Finzi
    Insert
    T7A1, O2, O-, Lambda t1 in lacZ fragment
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7A1
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    170479-plasmid-map-sequence-id-336812

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  19. pCMVlac-dirTopo-AU1-aGFP

    Plasmid
    #61971
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Promoter
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61971-plasmid-map-sequence-id-101286

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  20. pUC-dirTopo-P2A-aGFP

    Plasmid
    #61974
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Promoter
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61974-plasmid-map-sequence-id-101282

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  21. pUC-dirTopo-AU1-aGFP

    Plasmid
    #61973
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Promoter
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61973-plasmid-map-sequence-id-101284

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  22. pET28GSTlinker-LIC

    Plasmid
    #119970
    Purpose
    Structure determination and a long linker aiming to keep GST tag free in DNA binding screening experiments.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 9, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119970-plasmid-map-sequence-id-232695

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  23. CFP(1-158)Stop in pcDNA1/amp

    Plasmid
    #60892
    Purpose
    This vector attaches CFP(1-158) to the C-terminus of a protein.
    Depositor
    Catherine Berlot
    Insert
    CFP(1-158)Stop
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    60892-plasmid-map-sequence-id-112569

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  24. Cerulean(1-158)Stop in pcDNA1/amp

    Plasmid
    #60894
    Purpose
    This vector attaches mCerulean(1-158) to the C-terminus of a protein.
    Depositor
    Catherine Berlot
    Insert
    Cerulean(1-158)Stop
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    60894-plasmid-map-sequence-id-112542

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  26. pET28 His-MBP-TEV-BAP-Human BCKDK

    Plasmid
    #232116
    Purpose
    For bacterial expression of Human BCKDK (AA31–412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag.
    Depositor
    Ruth Sommese
    Article
    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi
    Insert
    BCKDK (BCKDK Human)
    Use
    Tags
    6 x His, Biotin acceptor peptide (BAP), Maltose-B…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232116-plasmid-map-sequence-id-464924

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  27. pDmelOR-mRho.V5.mER.hOr56a

    Plasmid
    #126479
    Purpose
    Human codon-optimized D. melanogaster Orco (hOrco) and Or56a (hOr56a). Or56a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells
    Depositor
    Bill Hansson
    Article
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Inserts
    Or56a (Or56a Fly)
    Orco (Orco Fly)
    Use
    Tags
    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens and codon optim…
    Promoter
    CMV
    Available since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    126479-plasmid-map-sequence-id-246521

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  28. G protein alpha-s-mCerulean

    Plasmid
    #66084
    Purpose
    G protein alpha-s internally tagged with mCerulean and EE epitope
    Depositor
    Catherine Berlot
    Article
    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1
    Insert
    G-alpha-s-EE-mCerulean (Gnas Rat, Aequorea victoria)
    Use
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    BamHI sites in the alpha-s cDNA were removed and …
    Promoter
    CMV
    Available since
    Aug. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    66084-plasmid-map-sequence-id-118926

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  29. pCMVlac-dirTopo-P2A-aGFP

    Plasmid
    #61972
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Promoter
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61972-plasmid-map-sequence-id-101285

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  30. pCDH-EF1-Fon-tdTomato

    Plasmid
    #72261
    Purpose
    Express tdTomato when FLP is expressed by another vector
    Depositor
    Kazuhiro Oka
    Insert
    tdTomato
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    T230I in tdTomato (please see depositor's co…
    Promoter
    elongation factor-1
    Available since
    Jan. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    72261-plasmid-map-sequence-id-131822

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  31. YFP(159-238)Stop in pcDNA1/amp

    Plasmid
    #60891
    Purpose
    This vector attaches YFP(159-238) to the C-terminus of a protein.
    Depositor
    Catherine Berlot
    Insert
    YFP(159-238)Stop
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    60891-plasmid-map-sequence-id-112575

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  32. CFP(159-238)Stop in pcDNA1/amp

    Plasmid
    #60893
    Purpose
    This vector attached CFP(159-238) to the C-terminus of a protein.
    Depositor
    Catherine Berlot
    Insert
    CFP(159-238)Stop
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn-164 was changed to His
    Promoter
    CMV
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    60893-plasmid-map-sequence-id-118771

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  33. pGL004

    Plasmid
    #215336
    Purpose
    amyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank-Hy promoter)
    Depositor
    Gene-Wei Li
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    hyper-pSpank
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215336-plasmid-map-sequence-id-426101

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  34. pGL003

    Plasmid
    #215335
    Purpose
    amyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank promoter)
    Depositor
    Gene-Wei Li
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pSpank
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215335-plasmid-map-sequence-id-426095

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  35. AAV-SYN-eGFP-oPRE

    Plasmid
    #218681
    Purpose
    AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.
    Depositor
    Bart Nieuwenhuis
    Insert
    eGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218681-plasmid-map-sequence-id-433352

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  37. pDmelOR-mRho.V5.mER.hOr47a

    Plasmid
    #126478
    Purpose
    Human codon-optimized D. melanogaster Orco (hOrco) and Or47a (hOr47a). Or47a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells
    Depositor
    Bill Hansson
    Article
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Inserts
    Or47a (Or47a Fly)
    Orco (Orco Fly)
    Use
    Tags
    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens and codon optim…
    Promoter
    CMV
    Available since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    126478-plasmid-map-sequence-id-246565

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  38. pLXRN-EGFP

    Plasmid
    #99207
    Purpose
    EGFP expressing bicistronic retroviral vector
    Depositor
    Peter Pedersen
    Insert
    EGFP
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    A Kozak sequence was incorporated to enhance tran…
    Promoter
    LTR
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99207-plasmid-map-sequence-id-190957

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Showing: 1961 - 2000 of 64857 results