We narrowed to 14,145 results for: TIM
-
Plasmid#125810PurposeLevel0 nSpCas9-ABE7.10 base changer, human codon optimizedDepositorInsertnSpCas9-ABE7.10 base changer, human codon optimized
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLPCX mCER-Prx1
Plasmid#158227PurposeGenetically encoded HomoFRET-based probe to monitor peroxiredoxin1 (Prx1) oligomerization (for stable cell line generation)DepositorInsertmCER-Prx1
UseRetroviralExpressionMammalianAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJY3948
Plasmid#113338Purpose6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expressionDepositorInsert2-pyrone synthase, codon optimized for Y. lipolytica
Tags6XHisExpressionYeastMutationPlease see depositor commentsAvailable SinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLPCX mCER-Prx2
Plasmid#158225PurposeGenetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for stable cell line generation)DepositorInsertmCER-Prx2
UseRetroviralExpressionMammalianAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET10
Plasmid#107440PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorAvailable SinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomApple-CaURA3
Plasmid#44879PurposeYeast tagging vector to create gene fusion with yeast optimized mApple with CaUra3 selectionDepositorInsertyomApple
ExpressionYeastAvailable SinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoClover-SpHis5
Plasmid#44857PurposeYeast tagging vector to create gene fusion with yeast optimized Clover with SpHis5 selectionDepositorInsertyoClover
ExpressionYeastAvailable SinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomRuby-SpHis5
Plasmid#44843PurposeYeast tagging vector to create gene fusion with yeast optimized mRuby with SpHis5 selectionDepositorInsertyomRuby
ExpressionYeastAvailable SinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPglu-3.2mDelta13
Plasmid#13560DepositorInsertFLIPglu F16A S112A Delta13
TagsECFP and EYFPExpressionBacterialMutationF16A, S112A in binding proteinAvailable SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
BCJJ344
Plasmid#117503PurposeUBI10:Cas9:E9 Golden Gate Level 1 Position 2 reverseDepositorInsertBpiI:tagt:UBI10:Cas9-3(intron):E9:ttgc:BpiI
UseCRISPRExpressionPlantPromoterAtUBI10Available SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB1-Clover
Plasmid#103991PurposePlasmid encoding CloverDepositorInsertClover
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (S30R, Y39N, …PromoterproCAvailable SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIS.MTRIA-CCK
Plasmid#184602PurposeFor expression of a fluorescent sensor for CCK in mammalian cellsDepositorInsertMTRIA-CCK
ExpressionMammalianPromoterCAG promoterAvailable SinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomWasabi-SpHis5
Plasmid#44850PurposeYeast tagging vector to create gene fusion with yeast optimized mWasabi with SpHis5 selectionDepositorInsertyomWasabi
ExpressionYeastAvailable SinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEBB-XIAP1-351
Plasmid#11560DepositorAvailable SinceApril 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLII275Pglu-4.6m
Plasmid#13565DepositorInsertFLIPglu F16A G275S-ECFP-K276R
TagsCitrineExpressionBacterialMutationF16A, ECFP insert in mglBAvailable SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFH67
Plasmid#131766PurposeLevel1 ZmUBIp::SpCas9 (wheat codon optimized, version 3):NOStDepositorInsertZmUBIp::SpCas9 (wheat codon optimized, version 3):NOSt
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
HIV-1 integrase F185H/C280S in pET15b
Plasmid#61672PurposeHIV-1 integrase F185H/C280S in pET-15bDepositorInsertHIV-1 integrase F185H/C280S
UseSynthetic BiologyTagsHisPromoterT7 promotorAvailable SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
KA1390_pGL4-UBCmin-luc2
Plasmid#124185PurposeLuciferase reporter with the UBC minimal promoterDepositorTypeEmpty backboneUseLuciferaseAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPSmOrange-SpHis5
Plasmid#44856PurposeYeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with SpHis5 selectionDepositorInsertyoPSmOrange
ExpressionYeastAvailable SinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEB1-Venus SX
Plasmid#103990PurposePlasmid encoding Venus SXDepositorInsertVenus SX
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F46L, F64L, …PromoterproCAvailable SinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mVenus JBC
Plasmid#103987PurposePlasmid encoding mVenus JBCDepositorInsertmVenus JBC
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F46L, F64L, …PromoterproCAvailable SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPglu-2uDelta13
Plasmid#13561DepositorInsertFLIPglu F16F D152A Delta13
TagsECFP and EYFPExpressionBacterialMutationF16A, D152A in binding proteinAvailable SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pVHed02
Plasmid#82352PurposeBxb1/TP901 temporal logic gate with mKate2 and sfGFP on low-copy plasmidDepositorInsertsmKate2 RFP
Superfolder GFP
Temporal logic gate with TP901 and Bxb1 attachment sites
UseSynthetic BiologyExpressionBacterialAvailable SinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pN_35S/mApple
Plasmid#132566PurposemApple expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)DepositorInsertmApple
ExpressionPlantPromoterCauliflower mosaic virus 35SAvailable SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUAS-MmTFP13xmyc
Plasmid#86371Purposemembrane targeted Tfp1 expression in zebrafishDepositorInsertmTfp1
UseZebrafishTags3xMyc and GAP43 membrane targetingPromoterUASAvailable SinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-dH3-YFP
Plasmid#8694DepositorAvailable SinceJune 30, 2005AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPS-CFP2-SpHis5
Plasmid#44853PurposeYeast tagging vector to create gene fusion with yeast optimized photoconvertible CFP2 with SpHis5 selectionDepositorInsertyoPS-CFP2
ExpressionYeastAvailable SinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPS-CFP2-Kan
Plasmid#44948PurposeYeast tagging vector to create gene fusion with yeast optimized photoconvertible CFP2 with Kan selectionDepositorInsertyoPS-CFP2
ExpressionYeastAvailable SinceOct. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAV-oChEF-citrine
Plasmid#50975PurposeAAV transfer vector with mammalian codon-optimized ChEF-citrine between AAV2 ITRDepositorInsertChEF-citrine
UseAAVTagscitrineMutationChEF gene is mammalian codon optimizedPromoterhuman synapsin promoterAvailable SinceFeb. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET17
Plasmid#107443PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorAvailable SinceApril 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBD21_033
Plasmid#109150PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of SiriusDepositorInsertsmRFP
Sirius
UseSynthetic BiologyExpressionBacterial and MammalianPromoterCMV and pTetAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBD21_036
Plasmid#109153PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of mKO2DepositorInsertsmRFP
mKO2
UseSynthetic BiologyExpressionBacterial and MammalianPromoterCMV and pTetAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBD21_034
Plasmid#109151PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of TagBFPDepositorInsertsmRFP
TagBFP
UseSynthetic BiologyExpressionBacterial and MammalianPromoterCMV and pTetAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
CyclinB-GFP
Plasmid#54465PurposeCyclinB-GFP in pIVT vector for in vitro transcriptionDepositorInsertCyclinB
TagsGFPExpressionMammalianMutationK67E and I150T mutations compared to GenBank refe…Available SinceJan. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoEmerald-Kan
Plasmid#44946PurposeYeast tagging vector to create gene fusion with yeast optimized Emerald with Kan selectionDepositorInsertyoEmerald
ExpressionYeastAvailable SinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-lifeact-mAPPLE
Plasmid#89820PurposemAPPLE labeled fluorescent actinDepositorInsertlifeact-mAPPLE
UseRetroviralAvailable SinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
YeNL-H2B_pcDNA3
Plasmid#89522PurposeYellow color variant of bright luminescent protein, fused to histone 2BDepositorInsertYeNL
Tagshistone 2BExpressionMammalianPromoterCMVAvailable SinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
BCJJ340
Plasmid#117502PurposeUBI10:Cas9:E9 Golden Gate Level 1 Position 2DepositorInsertBpiI:GCAA:UBI10:Cas9-3(intron):E9:ACTA:BpiI
UseCRISPRExpressionPlantPromoterAtUBI10Available SinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
csr-1_son-1_mScarlet
Plasmid#191745PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
TagsNCU04502 promoter and mScarletExpressionBacterialAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVD_85
Plasmid#149604Purpose(parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - XylRDepositorInsertXylR
UseSynthetic BiologyTagsSV40ExpressionYeastPromoterTEF1Available SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup133-1
Plasmid#87334PurposeTo express shRNA against human Nup133DepositorInsertshRNA against human Nup133
UseRNAiExpressionMammalianPromoterH1Available SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpG-P2A-mScarlet (MNW006)
Plasmid#174136PurposeCMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarletDepositorInserthuman codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-mScarletExpressionMammalianMutationSpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337RPromoterCMV and T7Available SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet17
Plasmid#107453PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
csr-1_son-1_mApple
Plasmid#191741PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
csr-1_son-1_mCherry
Plasmid#191742PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
TagsNCU04502 promoter and mCherryExpressionBacterialAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
tubulin-CeNL_pcDNA3
Plasmid#89515PurposeCyan color variant of bright luminescent protein, fused to b-tubulinDepositorInsertCeNL
Tagsb-tubulinExpressionMammalianPromoterCMVAvailable SinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2-7SK-Blast
Plasmid#183620PurposelentiCRISPRv2 derivative with modified 7SK promoter driving sgRNA and encoding Blasticidin resistance. Compatible with inserts designed for lentiCRISPRv2.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterModified 7SKAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
YeNL-Actin_pcDNA3
Plasmid#89521PurposeYellow color variant of bright luminescent protein, fused to actinDepositorInsertYeNL
TagsActinExpressionMammalianPromoterCMVAvailable SinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNB777
Plasmid#60153PurposeRed click beetle luciferase (Promega) driven by LEU1 promoter.DepositorInsertCBR
ExpressionYeastPromoterLEU1prAvailable SinceNov. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L4-pTF-L1R
Plasmid#45954DepositorInsertpTF promoter
UseGateway donor vectorPromoterTET optimizedAvailable SinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only